LOCUS AK291024 3646 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77931 complete cds.
ACCESSION AK291024
VERSION AK291024.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3646)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3646
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3003881"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423821"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 101..2854
/codon_start=1
/protein_id="BAF83713.1"
/transl_table=1
/translation="MFAVHLMAFYFSKLKEDQIKKVDRFLYHMRLSDDTLLDIMRRFR
AEMEKGLAKDTNPTAAVKMLPTFVRAIPDGSENGEFLSLDLGGSKFRVLKVQVAEEGK
RHVQMESQFYPTPNDIIRGNGTELFEYVADCLADFMKTKDLKHKKLPLGLTFSFPCRQ
TKLEEGVLLSWTKKFKARGVQDTDVVSRLTKAMRRHKDMDVDILALVNDTVGTMMTCA
YDDPYCEVGVIIGTGTNACYMEDMSNIDLVEGDEGRMCINTEWGAFGDDGALEDIRTE
FDRELDLGSLNPGKQLFEKMISGLYLGELVRLILLKMAKAGLLFGGEKSSALHTKGKI
ETRHVAAMEKYKEGLANTREILVDLGLEPSEADCIAVQHVCTIVSFRSANLCAAALAA
ILTRLRENKKVERLRTTVGMDGTLYKIHPQYPKRLHKVVRKLVPSCDVRFLLSESGST
KGAAMVTAVASRVQAQRKQIDRVLALFQLTREQLVDVQAKMRAELEYGLKKKSHGLAT
VRMLPTYVCGLPDGTEKGKFLALDLGGTNFRVLLVKIRSGRRSVRMYNKIFAIPLEIM
QGTGEELFDHIVQCIADFLDYMGLKGASLPLGFTFSFPCRQMSIDKGTLIGWTKGFKA
TDCEGEDVVDMLREAIKRRNEFDLDIVAVVNDTVGTMMTCGYEDPNCEIGLIAGTGSN
MCYMEDMRNIEMVEGGEGKMCINTEWGGFGDNGCIDDIRTRYDTEVDEGSLNPGKQRY
EKMTSGMYLGEIVRQILIDLTKQGLLFRGQISERLRTRGIFETKFLSQIESDRLALLQ
VRRILQQLGLDSTCEDSIVVKEVCGAVSRRAAQLCGAGLAAIVEKRREDQGLEHLRIT
VGVDGTLYKLHPHFSRILQETVKELAPRCDVTFMLSEDGSGKGAALITAVAKRLQQAQ
KEK"
BASE COUNT 891 a 851 c 1094 g 810 t
ORIGIN
1 ctgccagcct ggactggaag cgtgcaacac tccagagtcg taggagtgaa cactgcacag
61 gaatctctgc ccatctcagg agaaaccaaa cttggggaaa atgtttgcgg tccacttgat
121 ggcattttac ttcagcaagc tgaaggagga ccagatcaag aaggtggaca ggttcctgta
181 tcacatgcgg ctctccgatg acaccctttt ggacatcatg aggcggttcc gggctgagat
241 ggagaagggc ctggcaaagg acaccaaccc cacggctgca gtgaagatgt tgcccacctt
301 cgtcagggcc attcccgatg gttccgaaaa tggggagttc ctttccctgg atctcggagg
361 gtccaagttc cgagtgctga aggtgcaagt cgctgaagag gggaagcgac acgtgcagat
421 ggagagtcag ttctacccaa cgcccaatga tatcatccgc gggaacggca cagagctgtt
481 tgaatatgta gctgactgtc tggcagattt catgaagacc aaagatttaa agcataagaa
541 attgcccctt ggcctaactt tttctttccc ctgtcgacag actaaactgg aagagggtgt
601 cctactttcg tggacaaaaa agtttaaggc acgaggagtt caggacacgg atgtggtgag
661 ccgtctgacc aaagccatga gaagacacaa ggacatggac gtggacatcc tggccctggt
721 caatgacacc gtggggacca tgatgacctg tgcctatgac gacccctact gcgaagttgg
781 tgtcatcatc ggaactggca ccaatgcgtg ttacatggag gacatgagca acattgacct
841 ggtggagggc gacgagggca ggatgtgcat caacacagag tggggggcct tcggggacga
901 cggggccctg gaggacattc gcactgagtt cgacagggag ctggacctcg gctctctcaa
961 cccaggaaag caactgttcg agaagatgat cagtggcctg tacctggggg agcttgtcag
1021 gcttatcttg ctgaagatgg ccaaggctgg cctcctgttt ggtggtgaga aatcttctgc
1081 tctccacact aagggcaaga tcgaaacacg gcacgtggct gccatggaga agtataaaga
1141 aggccttgct aatacaagag agatcctggt ggacctgggt ctggaaccgt ctgaggctga
1201 ctgcattgcc gtccagcatg tctgtaccat cgtctccttc cgctcggcca atctctgtgc
1261 agcagctctg gcggccatcc tgacacgcct ccgggagaac aagaaggtgg aacggctccg
1321 gaccacagtg ggcatggacg gcaccctcta caagatacac cctcagtacc caaaacgcct
1381 gcacaaggtg gtgaggaaac tggtcccaag ctgtgatgtc cgcttcctcc tgtcagagag
1441 tggcagcacc aagggggccg ccatggtgac cgcggtggcc tcccgcgtgc aggcccagcg
1501 gaagcagatc gacagggtgc tggctttgtt ccagctgacc cgagagcagc tcgtggacgt
1561 gcaggccaag atgcgggctg agctggagta tgggctgaag aagaagagcc acgggctggc
1621 cacggtcagg atgctgccca cctacgtctg cgggctgccg gacggcacag agaaaggaaa
1681 gtttctcgcc ctggatcttg ggggaaccaa cttccgggtc ctcctggtga agatcagaag
1741 tggacggagg tcagtgcgaa tgtacaacaa gatcttcgcc atccccctgg agatcatgca
1801 gggcactggt gaggagctct ttgatcacat tgtgcagtgc atcgccgact tcctggacta
1861 catgggcctc aagggagcct ccctaccttt gggcttcaca ttctcatttc cctgcaggca
1921 gatgagcatt gacaagggaa cactcatagg gtggaccaaa ggtttcaagg ccactgactg
1981 tgaaggggag gacgtggtgg acatgctcag ggaagccatc aagaggagaa acgagtttga
2041 cctggacatt gttgcagtcg tgaatgatac agtggggacc atgatgacct gtggctatga
2101 agatcctaat tgtgagattg gcctgattgc aggaacaggc agcaacatgt gctacatgga
2161 ggacatgagg aacatcgaga tggtggaggg gggtgaaggg aagatgtgca tcaatacaga
2221 gtggggagga tttggagaca atggctgcat agatgacatc cggacccgat acgacacgga
2281 ggtggatgag gggtccttga atcctggcaa gcagagatac gagaaaatga ccagtgggat
2341 gtacttgggg gagattgtgc ggcagatcct gatcgacctg accaagcagg gtctcctctt
2401 ccgagggcag atttcagagc gtctccggac caggggcatc ttcgaaacca agttcctgtc
2461 ccagatcgaa agcgatcggc tggcccttct ccaggtcagg aggattctgc agcagctggg
2521 cctggacagc acgtgtgagg acagcatcgt ggtgaaagag gtgtgcggag ccgtgtcccg
2581 gcgggcggcc cagctctgcg gtgctggcct ggccgctata gtggaaaaaa ggagagaaga
2641 ccaggggcta gagcacctga ggatcactgt gggtgtggac ggcaccctgt acaagctgca
2701 ccctcacttt tctagaatat tgcaggaaac tgtgaaggaa ctagcccctc gatgtgatgt
2761 gacattcatg ctgtcagaag atggcagtgg aaaaggggca gcactgatca ctgctgtggc
2821 caagaggtta cagcaggcac agaaggagaa ataggaaccc ctgggattgg acctgatgca
2881 tcttggatac tgaacagctt ttcctctggc agatcagttg gccagagacc aatgggcacc
2941 ctcctggctg acctcacctt ctggatggcc gaaagagaac cccaggttct cgggtactct
3001 tagtatcttg tactggattt gcagtgacat tacatgacat ctctatttgg tatatttggg
3061 ccaaaatggg ccaacttatg aaatcaaagt gtctgtcctg agagatcccc tttcaacaca
3121 ttgttcaggt gaggcttgag ctgtcaattc tctatggctt tcagtcttgt ggctgcggga
3181 cttggaaata tatagaatct gcccatgtgg ctggcaggct gtttccccat tgggatgctt
3241 aagccatctc ttatagggga ttggaccctg tacttgtgga tgaacattgg agagcaagag
3301 gaactcacgt tatgaactag ggggatctca tctaacttgt ccttaacttg ccatgttgac
3361 ttcaaacctg ttaagagaac aaagactttg aagtatccag ccccagggtg cagagaggtt
3421 gattgccagg gagcactgca ggaatcattg catgcttaaa gcgagttatg tcagcaccct
3481 gtaggatttt gttccttatt aagtgtgtgc catgtggtgg ggtgctgtct ggggcatctg
3541 tttttcattt tgcctgtggt ttgtgttgca ggtgttgata gttgttttaa ggattgttag
3601 gtataggaaa tccagtaaat taataaaaaa attttgattt tccaat
//