LOCUS AK291022 1799 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ78561 complete cds, highly similar to Homo
sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript
variant 1, mRNA.
ACCESSION AK291022
VERSION AK291022.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1799)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1799
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3003560"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423819"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 142..1665
/codon_start=1
/note="highly similar to Homo sapiens neuro-oncological
ventral antigen 1 (NOVA1), transcript variant 1, mRNA"
/protein_id="BAF83711.1"
/transl_table=1
/translation="MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNT
GEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLI
QGTVEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQTLPSSPTTTKS
SPSDPMTTSRANQVKIIVPNSTAGLIIGKGGATVKAVMEQSGAWVQLSQKPDGINLQE
RVVTVSGEPEQNRKAVELIIQKIQEDPQSGSCLNISYANVTGPVANSNPTGSPYANTA
EVLPTAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAITSALNTLASYGYNLNTLGL
GLSQAAATGALAAAAASANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAAT
AATNGYFGAASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQEL
TGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRAANPQKVG"
BASE COUNT 566 a 426 c 420 g 387 t
ORIGIN
1 tatattgatc cgattaaaaa aaaaaaacct taagaaaccc caaacgcaaa aaaaaaaaaa
61 aaaaaaaaaa gaaaaaagaa aagaaaaagc caaaacaaaa gggagaacct tctcccggta
121 gcagcggcag gaactgcaaa catgatggcg gcagctccca tccagcagaa cgggacccac
181 actggggttc ccatagacct ggacccgccg gactcgcgga aaaggccgct ggaagccccc
241 cctgaagccg gcagcaccaa gaggaccaat acgggcgaag acggccagta ttttctaaag
301 gttctcatac ctagttatgc tgctggatct ataattggga agggaggaca gacaattgtt
361 cagttgcaaa aagaaactgg agccaccatc aagctgtcta agtccaaaga tttttaccca
421 ggtactactg agcgagtgtg cttgatccag ggaacggttg aagcactgaa tgcagttcat
481 ggattcattg cagaaaaaat tcgagaaatg ccccaaaatg tggccaagac agaaccagtc
541 agcattctac aaccccagac caccgttaat ccagatcgca tcaaacaaac attgccatct
601 tccccaacta ccaccaagtc ctctccatct gatcccatga ccacctccag agctaatcag
661 gtaaagatta tagttcccaa cagcacagca ggtctgataa tagggaaggg aggtgctact
721 gtgaaggctg taatggagca gtcaggggct tgggtgcagc tttcccagaa acctgatggg
781 atcaacttgc aagagagggt tgtcactgtg agtggagaac ctgaacaaaa ccgaaaagct
841 gttgaactta tcatccagaa gatacaagag gatccacaaa gtggcagctg tctcaatatc
901 agttatgcca atgtgacagg tccagtggca aattccaatc caaccggatc tccttatgca
961 aacactgctg aagtgttacc aactgctgca gcagctgcag ggctattagg acatgctaac
1021 cttgctggcg ttgcagcctt tccagcagtt ttatctggct tcacaggcaa tgacctggtg
1081 gccatcacct ctgcacttaa tacattagcc agctatggat ataatctcaa cactttaggt
1141 ttaggtctca gtcaagcagc agcaacaggg gctttggctg cagcagctgc cagtgccaac
1201 ccagcagcag cagcagccaa tttattggcc acctatgcca gtgaagcctc agccagtggc
1261 agcacagctg gtggtacggc ggggacattt gcattaggta gcctggctgc tgctactgct
1321 gcaaccaatg gatattttgg agctgcttct cccctagctg ccagtgccat tctaggaaca
1381 gaaaagtcca cagatggatc caaggatgta gttgaaatag cagtgccaga aaacttagtt
1441 ggtgcaatac ttggcaaagg agggaaaaca ttagtggaat accaggagtt gactggtgca
1501 aggatacaga tctccaaaaa aggagaattc gtacctggca caaggaatcg gaaggtaacc
1561 attactggaa caccagctgc aacacaggct gctcaatatt taattacaca aaggatcaca
1621 tatgagcaag gagttcgggc tgccaatcct cagaaagtgg gttgagtgcc ccagttacac
1681 atcagattgt tttaacccct cctttacccc attttcaaga aggatgtact gtactttgca
1741 gaagtgaagt ttttctgtta ttaatatata attatgcaaa tgaatgcgac tatgttgac
//