LOCUS AK291008 1639 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76172 complete cds.
ACCESSION AK291008
VERSION AK291008.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1639)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1639
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3001359"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423805"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 17..1372
/codon_start=1
/protein_id="BAF83697.1"
/transl_table=1
/translation="MAEAAALVWIRGPGFGCKAVRCASGRCTVRDFIHRHCQDQNVPV
ENFFVKCNGALINTSDTVQHGAVYSLEPRLCGGKGGFGSMLRALGAQIEKTTNREACR
DLSGRRLRDVNHEKAMAEWVKQQAEREAEKEQKRLERLQRKLVEPKHCFTSPDYQQQC
HEMAERLEDSVLKGMQAASSKMVSAEISENRKRQWPTKSQTDRGASAGKRRCFWLGME
GLETAEGSNSESSDDDSEEAPSTSGMGFHAPKIGSNGVEMAAKFPSGSQRARVVNTDH
GSPEQLQIPVTDSGRHILEDSCAELGESKEHMESRMVTETEETQEKKAESKEPIEEEP
TGAGLNKDKETEERTDGERVAEVAPEERENVAVAKLQESQPGNAVIDKETIDLLAFTS
VAELELLGLEKLKCELMALGLKCGGTLQERAARLFSVRGLAKEQIDPALFAKPLKGKK
K"
BASE COUNT 468 a 325 c 470 g 376 t
ORIGIN
1 tcgggtagtc ggtgacatgg cggaggccgc ggcgctggtg tggattcgcg gccctggctt
61 cgggtgcaag gcggtgcggt gtgcctcggg tcggtgcacc gtccgggatt ttatccaccg
121 gcactgccaa gatcagaatg ttccagtgga aaacttcttt gtgaaatgca atggagcact
181 cattaacacc agtgacacag tgcagcatgg agctgtttat agtttggaac ccagactttg
241 cggtggaaaa ggaggttttg gatctatgct ccgagcactt ggtgctcaga ttgagaagac
301 aaccaatcga gaagcttgtc gggatctcag tggaaggaga ctacgcgatg tcaatcatga
361 aaaagcaatg gctgaatggg taaaacaaca agccgagcga gaggctgaaa aggagcagaa
421 gcggctggag cgactgcagc ggaagcttgt agaacccaag cactgcttca ccagccccga
481 ctaccagcag cagtgccatg agatggctga gcgtctggag gattccgtcc tcaaaggtat
541 gcaggctgcc tccagcaaga tggtttcagc agaaatcagt gagaatcgga aacggcaatg
601 gcctactaaa tctcaaacag acagaggagc cagtgcggga aagaggagat gcttctggtt
661 gggcatggag ggactagaga ctgcagaagg gtccaactct gagagttcag atgatgacag
721 tgaagaagca ccaagcactt caggaatggg tttccatgct ccaaaaattg gtagcaatgg
781 tgtcgagatg gcagccaaat ttcccagtgg ttctcagagg gcgagagtag tgaatacaga
841 ccatggatca ccagaacaac tgcagatccc ggtgactgac tctgggaggc atattttaga
901 agactcatgt gctgagctgg gggagtccaa agagcacatg gaaagcagga tggttacaga
961 aacagaagag acccaggaga agaaggcaga gagtaaagaa cccatagaag aggagcccac
1021 tggggctgga ctgaataagg ataaagagac agaagaaagg actgatgggg aaagagttgc
1081 tgaggtagca cctgaagaaa gggaaaacgt tgccgttgcc aaactgcagg aaagccagcc
1141 aggaaacgca gttattgata aggaaactat agatttattg gcgttcacct ctgttgcaga
1201 actggagttg ctgggtttgg agaagctcaa atgtgaactg atggcccttg gactgaaatg
1261 tgggggcact ctgcaggagc gggcagcaag actcttctct gtcagaggac tggcaaagga
1321 gcaaattgac ccggctttat ttgccaagcc tttgaaaggg aagaaaaaat gagtatcatc
1381 agagctgatt cctatttgtt tatagtgtct accatttaca acctgcataa tgtggactct
1441 tgaccagtat tcctgttgat attaaaagct aactttttag taacccaatt ctaactatcc
1501 cctttttcct tgtcagggct gtagaattaa tgtctgaaac catctgggcc ttaaattttc
1561 tttgtctggt tttctatagt gtatttaata aatatagggc tattcacatt ttttggtttg
1621 atcttggtgt catttaggt
//