LOCUS AK291007 3745 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76800 complete cds, highly similar to Homo
sapiens adducin 2 (beta) (ADD2), transcript variant beta-1, mRNA.
ACCESSION AK291007
VERSION AK291007.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3745)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3745
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3001214"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423804"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 467..2647
/codon_start=1
/note="highly similar to Homo sapiens adducin 2 (beta)
(ADD2), transcript variant beta-1, mRNA"
/protein_id="BAF83696.1"
/transl_table=1
/translation="MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQD
FNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVF
PTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLR
VSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAA
RPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQK
CLGPTCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQ
EKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKT
KHKSEVEIPATVTAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQ
RSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLEMRNKIREQNRQ
DVKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQLTDQELE
EYKKEVERKKLELDGEKETAPEEPGSPAKSAPASPVQSPAKEAETKSPLVSPSKSLEE
GTKKTETSKAATTEPETTQPEGVVVNGREEEQTAEEILSKGLSQMTTSADTDVDTSKD
KTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES"
BASE COUNT 977 a 1025 c 1035 g 708 t
ORIGIN
1 catttcctcc cagcctcgtg cgggaaatgg ctttaattct gacggcaggg ctgtgaggga
61 ctagcgggaa cccgagcctt ttgtcaagga actgcggcgt cggtggccag tcaaccccgc
121 cgccgcggag ccgctgcact gctgggggat ctcccagcag ctctgacgag cgcgggctgc
181 agcatgggca gaaaacgctg ctctgcagat tagctgggtg gattttttaa gcgcacccca
241 ccccccaaac ccataaaata acaaaaccaa cccgcagtgg ccgaccggag atagctaaga
301 tgccgcgcag gagtttccac ctggatgttt gaggttgtgt agatgtggcc ggcacccttg
361 agagtggagc tagggggtgc agactgagca gtgaacagaa ggagccttgg acagggctgg
421 gccagcctcc cgagttccag gagcgaattg caaacccacc gggaaaatga gcgaagagac
481 ggtccccgag gctgcctcgc cgccgccccc gcaggggcag ccttactttg accgcttctc
541 agaggacgac cccgagtaca tgcgccttcg caaccgggcg gcggacctgc ggcaggactt
601 caacctgatg gagcagaaga agcgcgtcac catgatcctg cagagtccct ctttcaggga
661 ggagctggaa ggcctcatcc aggagcagat gaagaagggg aacaactcct ccaacatctg
721 ggccctgcga cagatcgcgg acttcatggc cagcacctcc cacgcagtct tcccgacatc
781 ttccatgaat gtctccatga tgacgcctat caatgacctc cacacagctg actccctgaa
841 cctggccaaa ggggagcggc tcatgcggtg caagatcagc agtgtctacc gactcctgga
901 cctctatggc tgggcccagc tgagtgacac ctatgtcacg ttgagagtca gcaaggagca
961 ggaccacttc ctgatcagcc ctaagggagt ttcttgcagt gaagtcacag cgtccagcct
1021 gatcaaggtg aacattctgg gagaggtggt ggagaagggc agcagctgct tcccagtgga
1081 caccacaggc ttctgtctgc actcggccat ctatgcagcg aggcccgacg tgcgctgcat
1141 catccacctg cacacaccgg ccacagcagc ggtgtcggcc atgaagtggg gcctcctgcc
1201 tgtctcccac aatgccctgc tggtggggga catggcctat tatgacttca atggggaaat
1261 ggagcaggaa gccgatcgga tcaacctgca gaagtgcctt ggacccacct gcaagatcct
1321 ggtgctaaga aaccatggag tggttgctct gggtgacacg gtagaggagg cattttacaa
1381 gatcttccac ctgcaggctg catgtgagat acaggtgtcg gctctgtcca gtgccggggg
1441 agtggagaac ctcatcctcc tggagcagga gaagcaccgg ccccatgagg tgggctccgt
1501 gcagtgggcc gggagcacct ttgggcctat gcagaagagt cggctggggg agcatgagtt
1561 tgaggccctc atgaggatgc tggacaacct gggctacaga acaggttaca cgtatcgcca
1621 cccctttgtt caagagaaaa ccaaacacaa aagtgaggtg gagattccag ccacggtcac
1681 agccttcgtg tttgaggagg acggtgcccc ggtgcccgcc ctgcgacagc atgcccagaa
1741 gcagcagaag gagaagaccc gctggctcaa tacgcccaac acctacctgc gggtcaatgt
1801 ggccgatgag gtccagagga gcatgggcag cccccgaccc aagaccacgt ggatgaaggc
1861 tgacgaggtg gagaaatcca gcagtggcat gccgattcgc atcgaaaacc caaaccaatt
1921 tgtgcctctc tatactgacc cccaggaagt actggagatg aggaacaaga ttcgagaaca
1981 aaaccgacaa gatgtgaagt cagcggggcc tcagtcccag ctcctggcga gcgtcattgc
2041 cgagaagagc cgaagcccgt ctacagagag ccagctgatg tccaagggag acgaggatac
2101 caaagacgat tcagaggaga cggtgcccaa ccccttcagc caactcactg accaggagtt
2161 ggaggagtac aagaaagagg tggagaggaa gaaactagaa cttgatggag agaaagaaac
2221 tgccccagaa gagcctggct cacctgcaaa gtctgcacct gcttctccag tgcagagccc
2281 agcgaaggag gcagagacaa agagcccttt agtctctcct tccaagtctt tagaggaagg
2341 tactaagaag acagaaacaa gcaaagccgc caccacagag cccgaaacaa cccagccgga
2401 aggggtggtg gtcaacggga gggaggagga gcagacggca gaggaaatcc tcagcaaagg
2461 cctgagccag atgaccacca gtgctgacac ggatgttgat acctctaagg acaaaaccga
2521 gtcggtcacc agcggcccca tgtccccaga gggctcacct tccaagtctc cctcaaagaa
2581 gaaaaagaaa ttccgaaccc cctccttcct gaaaaagagc aaaaagaagg agaaagttga
2641 gtcctgattc atgacaccct tgggctccct cctgcctcct ctctctcctc cccttccctt
2701 ctcccatctc tgtccctgca agcacagggc taaggaggga tagagtagga ccctggacca
2761 cattcggaag gggaacttag agatcacccg accaaccctt cgttttacag ttgcccaaga
2821 gaaatcagat tacctgagac tgggtcattt ataatgaaca cagatttatt tctctcacat
2881 ctggaagctg ggaagtccaa gatcaaggca ccagcatttg gtgaagcctt ctttctgtgt
2941 catcccatgg cagaagacag aagggcaaga gaggaaaccg gtccaagcag gatgccttcc
3001 tttttctgac gggcagcccc cagcagtacc atcacaaacc ttggtctctt cttgtcacca
3061 gcaaagggat gaaggataag tgatgaggag actgaagcac agaggtgaag cagcttgccc
3121 aacgtcacac tgctgcttag tgatagaggc agtgctagac cccggggctc ctgaatgtta
3181 ctgttggagg ctcttaaccc actgggatca agaaggtgtc aagaactaga ctctccctcc
3241 aggagaatga acacagacac aaaaccttgc atatatgctt ccgggtaaag aacctgcctg
3301 cattttggta ccactacgtg ctggcctgtc ctgggcatgt tatatcatga gaaggcaggg
3361 aaggctgaat cattaaggac atgaatcaga ttgattctcg tggctttaaa aacaaacact
3421 acaggaccag catgacccgc cagcctgtta tccacagagg cttgtggtct ccattgaaaa
3481 gaagcaaaga cagcacgtcc caactcaaaa gattgtgtca tcctaaaggt gaaaggaaga
3541 gatgatccaa gcaaatttag gaccaatagc agactcttca cactgcctgg ggatagaggg
3601 accaccctgg gctcctctta ctcctggatt tacttggatg accagactca ggacttcatt
3661 taaatagaat ttggtttcat aatggaatct tcagcttgaa atataaattt gtttctgtct
3721 ccctctgcca tctcagcctt tcttg
//