LOCUS AK291006 2463 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76171 complete cds.
ACCESSION AK291006
VERSION AK291006.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2463)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2463
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3000789"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000423803"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 275..2020
/codon_start=1
/protein_id="BAF83695.1"
/transl_table=1
/translation="MEDVNSNVNADQEVRKLQELVKKLEKQNEQLRSRSGAVQGAGSL
GPGSPVRAGASIPSSGAASPRGFPLGLSAKSGGGPGSGPRRTSSEELRDATSLLAAGE
GGLLDEVEPLRPDELERLSGWEEEEESWLYSSPKKKLTPMQKSVSPLVWCRQVLDYPS
PDVECAKKSLIHKLDQTMSALKRQNLYNNPFNSMSYTSPYSPNASSPYSSGFNSPSST
PVRPPIVKQLILPGDSGNLKSSDRNPPLSPQSSIDSELSASELDEDSIGSNYKLNDVT
DVQILARMQEESLRQEYAATTSRRSSGSSCNSTRRGTFSDQELDAQSLDDEDDNMHHA
VYPAVNRFSPSPRNSPRPSPKQSPRNSPRSRSPARGIEYSRVSPQPMISRLQQPRLSL
QGHPTDLQTSNVKNEEKLRRSLPNLSRTSNTQVDSVKSSRSDSNFQVPNGGIPRMQPQ
ASAIPSPGKFRSPAAPSPLALRQPVKAFSNHGSGSPGSQEITQLTQTTSSPGPPMVQS
TVSANPPSNINSATLTRPAGTTAMRSGLPRPSAPSAGGIPVPRSKLAQPARRSLPAPK
TYGSMKDDSWKDGCY"
BASE COUNT 660 a 645 c 614 g 544 t
ORIGIN
1 gcggggggcg gagggacgga gggaagatgg cggcaggggc cggatattgg cgccgcctcc
61 cccaatcccg gagccggcgc agatgaggca gttcggctgg ggccagcggc gctttggaac
121 ccgaggtggg gggaccctgg cggtggggcc tggtcctgct atatgccggc gcctcggcta
181 gagtgagcgg cggcgacgcc tctttcctcc gtctctttcc ctgtcgctgc gagagcgagc
241 gggcggcgga ggcggcggcg gcggcggggc cgggatggag gacgttaact ccaacgtgaa
301 cgcggaccag gaggtgcgga agctgcagga gctggtgaag aagctggaga agcagaacga
361 acagctgagg agccgctcgg gggccgtgca gggcgccggc tcccttgggc ccggcagccc
421 ggttcgggcc ggcgcgtcca ttccctcctc cggcgcggcg tctcctcggg gcttcccctt
481 gggcctcagc gccaagtcgg gcggcgggcc cgggtcgggc ccgaggcgga cgagtagcga
541 agagctgcgg gacgccacct ccttgctagc ggcgggcgag ggcggcttgc tggacgaggt
601 ggagccgctg cggcccgacg agctggagcg cctgtcaggc tgggaggagg aggaggagag
661 ctggctgtat tcatcaccaa agaaaaaact tacaccaatg cagaaatcgg ttagtccatt
721 agtttggtgc aggcaagttt tggattaccc aagtcctgat gttgagtgtg ctaaaaaatc
781 tcttatccac aaacttgatc aaactatgtc agctctcaag aggcagaatt tatataataa
841 tcctttcaac tccatgagtt acaccagtcc ttacagtcca aatgccagta gcccatacag
901 cagtggcttc aattctccat cctcaacccc agtgcgacct cctatagtca aacagcttat
961 acttcctgga gattcaggta acttgaaaag ctcagacaga aatcctccac tcagtcctca
1021 gtcctctata gatagtgagt taagtgcttc agaattagat gaagattcaa ttggatccaa
1081 ttataagcta aatgatgtaa ctgatgtaca gattctagcc cggatgcagg aagaaagtct
1141 ccggcaagaa tatgcagcca ccacgtctcg gcgcagttct ggttcatctt gcaattctac
1201 aagacggggt acttttagtg atcaggaact tgatgcacaa agtttagatg atgaagatga
1261 caatatgcat catgcagtat accctgctgt taacaggttt tcaccatcac cacgcaattc
1321 acctcgaccg tcacctaagc agtcacccag aaattcacct cgttcacgat ctcctgctcg
1381 gggaatagaa tatagtagag tgtccccaca gcctatgatt agccgcttac agcaacctcg
1441 cctttcactt caaggccatc ccacagattt acagacaagc aatgttaaaa atgaagaaaa
1501 actaagacgc agtcttccaa acctgtcccg aacatctaat acacaagttg actcagtgaa
1561 aagcagcaga agtgactcaa attttcaagt gccaaacgga ggaatacctc gtatgcaacc
1621 tcaggcttca gccatacctt ctccaggcaa attccgttcc cctgcagcac catctccttt
1681 ggctcttcgg caaccagtga aagcatttag taaccatggc tctggttctc ctggtagcca
1741 agaaataaca cagctcacac aaaccacctc ctcacctggg cctcctatgg ttcagagcac
1801 agtctcagca aatcctccca gcaatatcaa cagcgctact ctaaccagac ctgcagggac
1861 aactgcaatg agaagtggct tgcccagacc cagtgcccct tctgctgggg gcataccagt
1921 gcctcgcagc aaacttgcac agcctgctcg cagatccttg ccagctccta aaacctatgg
1981 tagcatgaaa gatgacagtt ggaaagatgg ctgttactga ccagcaaaga caagaatgca
2041 gaagtccacg gcttcatgga tacccttcac caggctaaaa aacaactttt atatgcagac
2101 tgttcagata agactcttgg gatttataaa atcccagccc tctctgtctt aattagcaca
2161 aaccgacaga gatcatcaaa cagcacttta atgacattta acatcagatg tgtttggtaa
2221 tcatacaatc actctccata gaatcacttt tagttttgtt taatagaaac taggttgatt
2281 tttaaaaaat atttgacaga ggccaatatc tgggcaaata cctaatggat gccaaagaga
2341 aatgcccatt tgtaaatgag aaaaatgccc atttgtgcac aagaaaatgg tgaattcagg
2401 ctatccaaaa cttcacattc aacctaattt actgtataaa tagtatcaat aaatatttgt
2461 gtt
//