LOCUS AK290996 1935 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76798 complete cds, highly similar to Homo
sapiens carboxypeptidase E (CPE), mRNA.
ACCESSION AK290996
VERSION AK290996.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1935)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1935
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2021919"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000423793"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 140..1570
/codon_start=1
/note="highly similar to Homo sapiens carboxypeptidase E
(CPE), mRNA"
/protein_id="BAF83685.1"
/transl_table=1
/translation="MAGRGGSALLALCGALAACGWLLGAEAQEPGAPAAGMRRRRRLQ
QEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEP
GEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPD
GFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKI
VDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDA
IFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSS
NCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANAT
ISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELE
SFSERKEEEKEELMEWWKMMSETLNF"
BASE COUNT 520 a 432 c 503 g 480 t
ORIGIN
1 agccggggaa ggtgaggcga gtagaggctg gtgcggaact tgccgccccc agcagcgccg
61 gcgggctaag cccagggccg ggcagacaaa agaggccgcc cgcgtaggaa ggcacggccg
121 gcggcggcgg agcgcagcga tggccgggcg agggggcagc gcgctgctgg ctctgtgcgg
181 ggcactggct gcctgcgggt ggctcctggg cgccgaagcc caggagcccg gggcgcccgc
241 ggcgggcatg aggcggcgcc ggcggctgca gcaagaggac ggcatctcct tcgagtacca
301 ccgctacccc gagctgcgcg aggcactcgt gtccgtgtgg ctgcagtgca ccgccatcag
361 caggatttac acggtggggc gcagcttcga gggccgggag ctcctggtca tcgagctgtc
421 cgacaaccct ggcgtccatg agcctggtga gcctgaattt aaatacattg ggaatatgca
481 tgggaatgag gctgttggac gagaactgct cattttcttg gcccagtacc tatgcaacga
541 ataccagaag gggaacgaga caattgtcaa cctgatccac agtacccgca ttcacatcat
601 gccttccctg aacccagatg gctttgagaa ggcagcgtct cagcctggtg aactcaagga
661 ctggtttgtg ggtcgaagca atgcccaggg aatagatctg aaccggaact ttccagacct
721 ggataggata gtgtacgtga atgagaaaga aggtggtcca aataatcatc tgttgaaaaa
781 tatgaagaaa attgtggatc aaaacacaaa gcttgctcct gagaccaagg ctgtcattca
841 ttggattatg gatattcctt ttgtgctttc tgccaatctc catggaggag accttgtggc
901 caattatcca tatgatgaga cgcggagtgg tagtgctcac gaatacagct cctccccaga
961 tgacgccatt ttccaaagct tggcccgggc atactcttct ttcaacccgg ccatgtctga
1021 ccccaatcgg ccaccatgtc gcaagaatga tgatgacagc agctttgtag atggaaccac
1081 caacggtggt gcttggtaca gcgtacctgg agggatgcaa gacttcaatt accttagcag
1141 caactgtttt gagatcaccg tggagcttag ctgtgagaag ttcccacctg aagagactct
1201 gaagacctac tgggaggata acaaaaactc cctcattagc taccttgagc agatacaccg
1261 aggagttaaa ggatttgtcc gagaccttca aggtaaccca attgcgaatg ccaccatctc
1321 cgtggaagga atagaccacg atgttacatc cgcaaaggat ggtgattact ggagattgct
1381 tatacctgga aactataaac ttacagcctc agctccaggc tatctggcaa taacaaagaa
1441 agtggcagtt ccttacagcc ctgctgctgg ggttgatttt gaactggagt cattttctga
1501 aaggaaagaa gaggagaagg aagaattgat ggaatggtgg aaaatgatgt cagaaacttt
1561 aaatttttaa aaaggcttct agttagctgc tttaaatcta tctatataat gtagtatgat
1621 gtaatgtggt ctttttttta gattttgtgc agttaatact taacattgat ttatttttta
1681 atcatttaaa tattaatcaa ctttccttaa aataaatagc ctcttaggta aaaatataag
1741 aacttgatat atttcattct cttatatagt attcattttc ctacctatat tacacaaaaa
1801 agtatagaaa agatttaagt aattttgcca tcctaggctt aaatgcaata ttcctggtat
1861 tatttacaat gcagaatttt ttgagtaatt ctagctttca aaaattagtg aagttctttt
1921 actgtaattg gtgac
//