LOCUS AK290993 1737 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ75086 complete cds, highly similar to Homo
sapiens carboxypeptidase A4, mRNA.
ACCESSION AK290993
VERSION AK290993.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1737)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1737
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2020124"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000423790"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 48..1313
/codon_start=1
/note="highly similar to Homo sapiens carboxypeptidase
A4, mRNA"
/protein_id="BAF83682.1"
/transl_table=1
/translation="MRWILFIGALIGSSICGQEKFFGDQVLRINVRNGDEISKLSQLV
NSNNLKLNFWKSPSSFNRPVDVLVPSVSLQAFKSFLRSQGLEYAVTIEDLQALLDNED
DEMQHNEGQERSSNNFNYGAYHSLEAIYHEMDNIAADFPDLARRVKIGHSFENRPMYV
LKFSTGKGVRRPAVWLNAGIHSREWISQATAIWTARKIVSDYQRDPAITSILEKMDIF
LLPVANPDGYVYTQTQNRLWRKTRSRNPGSSCIGADPNRNWNASFAGKGASDNPCSEV
YHGPHANSEVEVKSVVDFIQKHGNFKGFIDLHSYSQLLMYPYGYSVKKAPDAEELDKV
ARLAAKALASVSGTEYQVGPTCTTVYPASGSSIDWAYDNGIKFAFTFELRDTGTYGFL
LPANQIIPTAEETWLGLKTIMEHVRDNLY"
BASE COUNT 421 a 445 c 444 g 427 t
ORIGIN
1 ataaatacag cttgactcag ccactgtatg actgactccc cggggacatg aggtggatac
61 tgttcattgg ggcccttatt gggtccagca tctgtggcca agaaaaattt tttggggacc
121 aagttttgag gattaatgtc agaaatggag acgagatcag caaattgagt caactagtga
181 attcaaacaa cttgaagctc aatttctgga aatctccctc ctccttcaat cggcctgtgg
241 atgtcctggt cccatctgtc agtctgcagg catttaaatc cttcctgaga tcccagggct
301 tagagtacgc agtgacaatt gaggacctgc aggccctttt agacaatgaa gatgatgaaa
361 tgcaacacaa tgaagggcaa gaacggagca gtaataactt caactacggg gcttaccatt
421 ccctggaagc tatttaccac gagatggaca acattgccgc agactttcct gacctggcga
481 ggagggtgaa gattggacat tcgtttgaaa accggccgat gtatgtactg aagttcagca
541 ctgggaaagg cgtgaggcgg ccggccgttt ggctgaatgc aggcatccat tcccgagagt
601 ggatctccca ggccactgca atctggacgg caaggaagat tgtatctgat taccagaggg
661 atccagctat cacctccatc ttggagaaaa tggatatttt cttgttgcct gtggccaatc
721 ctgatggata tgtgtatact caaactcaaa accgattatg gaggaagacg cggtcccgaa
781 atcctggaag ctcctgcatt ggtgctgacc caaatagaaa ctggaacgct agttttgcag
841 gaaagggagc cagcgacaac ccttgctccg aagtgtacca tggaccccac gccaattcgg
901 aagtggaggt gaaatcagtg gtagatttca tccaaaaaca tgggaatttc aagggcttca
961 tcgacctgca cagctactcg cagctgctga tgtatccata tgggtactca gtcaaaaagg
1021 ccccagatgc cgaggaactc gacaaggtgg cgaggcttgc ggccaaagct ctggcttctg
1081 tgtcgggcac tgagtaccaa gtgggtccca cctgcaccac tgtctatcca gctagcggga
1141 gcagcatcga ctgggcgtat gacaacggca tcaaatttgc attcacattt gagttgagag
1201 ataccgggac ctatggcttc ctcctgccag ctaaccagat catccccact gcagaggaga
1261 cgtggctggg gctgaagacc atcatggagc atgtgcggga caacctctac taggcgatgg
1321 ctctgctctg tctacattta tttgtaccca cacgtgcacg cactgaggcc attgttaaag
1381 gagctctttc ctacctgtgt gagtcagagc cctctgggtt tgtggagcac acaggcctgc
1441 ccctctccag ccagctccct ggagtcgtgt gtcctggcgg tgtccctgca agaactggtt
1501 ctgccagcct gctcaatttt ggtcctgctg tttttgatga gccttttgtc tgtttctcct
1561 tccaccctgc tggctgggcg gctgcactca gcatcacccc ttcctgggtg gcatgtctct
1621 ctctacctca tttttagaac caaagaacat ctgagatgat tctctaccct catccacatc
1681 tagccaagcc agtgaccttg ctctggtggc actgtgggag acaccacttg tctttag
//