LOCUS AK290986 2297 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ75033 complete cds, highly similar to Homo
sapiens RAS and EF-hand domain containing (RASEF), mRNA.
ACCESSION AK290986
VERSION AK290986.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2297)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2297
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2016634"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000423783"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 4..2226
/codon_start=1
/note="highly similar to Homo sapiens RAS and EF-hand
domain containing (RASEF), mRNA"
/protein_id="BAF83675.1"
/transl_table=1
/translation="MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRP
ADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDS
EEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEP
RLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEH
KTRKDEKRKAEEALSDLRRQYETEVGDLQVTIKKLRKLEEQSKCVSQKEDVAALKKQI
YDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRAYTEDRNS
LERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFISH
SPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYD
SEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSE
GSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEF
RENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVT
CEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTY
GALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG
"
BASE COUNT 663 a 504 c 632 g 498 t
ORIGIN
1 gggatggagg cggatgggga cggagaggag ctggcccggc tgcgctcagt cttcgccgcc
61 tgcgacgcga accgctcggg gcgcctggag cgcgaggagt tccgggcact gtgcacggag
121 ctgcgggtgc ggccggccga cgccgaggca gtattccagc ggctggacgc cgaccgtgac
181 ggcgccatca ccttccagga gttcgcgcgt ggcttcctcg ggtccctccg cggggggcgg
241 cgccgggact ggggtcctct ggatcccgcg cccgccgtgt ctgaggcggg gccggagaca
301 cacgacagcg aggaggacga aggcgacgag gacgcggcgg cggcgctggc cacctcgtgc
361 ggcccggcga gtcccggccg ggcttggcag gatttccagg cgcgacttgg ggacgaagcc
421 aagttcattc ccagagaaga gcaagttagt accttgtacc aaaacatcaa ccttgtggag
481 ccaagattaa ttcagccata tgaacatgtt ataaagaact tcatccgtga gatcagactt
541 caaagcacag aaatggaaaa tttggccatt gcggtgaaga gagcccagga caaggcagct
601 atgcagttga gtgagttgga agaggaaatg gatcagagga ttcaggctgc agaacataag
661 acacggaaag acgaaaaacg caaagctgag gaagccctca gtgacctcag acgtcagtat
721 gaaactgaag taggagatct gcaggtgacc attaaaaagc taagaaagct cgaagaacaa
781 tcaaaatgcg taagtcaaaa ggaagatgtg gctgcattga aaaaacaaat ttatgattta
841 tcaatggaaa accagaaagt taagaaagac cttttagaag cacagacaaa catagccttt
901 cttcagagtg agttagatgc tttgaaaagt gattatgctg atcagagtct gaatactgaa
961 agggatctgg aaataatccg agcatacaca gaagatcgaa atagtcttga gaggcaaatt
1021 gaaatactcc aaacagctaa ccggaagcta catgacagta atgatggcct tagaagtgcc
1081 cttgaaaaca gttatagcaa gttcaacaga tctttgcata taaataatat ctcaccaggg
1141 aatacaattt ctagaagcag tcccaaattc attagtcatt cccctcaacc tctaggctat
1201 gacaggtcat cccgctcttc ctatgtggat gaggactgtg actccctggc cctctgtgat
1261 cctctgcaga ggacaaattg tgaagttgac agcctgcctg aaagctgctt cgacagcggc
1321 ttgtctacct tgagagatcc caatgagtat gactcagaag tggaatacaa gcaccagagg
1381 ggatttcaga ggtcacacgg ggtgcaggag agctttggag gtgatgcttc agacacagat
1441 gttcctgaca taagggatga agagacattt ggtttagaag atgtggcttc cgtcttagac
1501 tggaagcccc aagggtctgt tagtgaaggc agcattgtta gttcatcaag aaagcccatc
1561 tcagcactct cgccccagac agacctggta gatgacaacg ctaaatcttt tagctcacag
1621 aaggcttaca agattgtact tgctggggac gctgcagtgg ggaagtctag tttcctcatg
1681 agactttgca agaatgaatt tcgagaaaat ataagcgcca ccctgggagt tgatttccaa
1741 atgaaaaccc tcattgtgga tggagaacga acagttctgc agctctggga tacagctggt
1801 caggagagat tcagaagtat tgccaagtct tacttcagaa aggcagatgg tgttttgctg
1861 ctgtatgatg ttacatgtga gaaaagcttt cttaacatac gagaatgggt agatatgatt
1921 gaggatgcag cccatgagac tgttcccatt atgctggtag gaaacaaggc tgacattcgt
1981 gacactgctg ctacagaggg acaaaaatgt gtcccagggc actttggaga gaaactggcc
2041 atgacgtatg gggcattatt ctgtgaaaca agtgccaaag atggttctaa catagtggag
2101 gccgttctgc accttgctcg agaagtgaaa aagagaactg acaaggatga cagcagatcc
2161 attaccaatc taaccgggac caattccaaa aagtcaccac agatgaagaa ttgttgcaat
2221 ggctaaatcc caaacatcct tggcctgtga agtcttcatt tccagaatac tgaatttgtg
2281 tgacttattt ggctctt
//