LOCUS AK290971 2350 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ78033 complete cds, highly similar to Homo
sapiens protein disulfide isomerase family A, member 4, mRNA.
ACCESSION AK290971
VERSION AK290971.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2350)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2350
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2006002"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000423768"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 68..2005
/codon_start=1
/note="highly similar to Homo sapiens protein disulfide
isomerase family A, member 4, mRNA"
/protein_id="BAF83660.1"
/transl_table=1
/translation="MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEE
EEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYG
KIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEI
VAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEK
AAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIV
DYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDY
KFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKY
ALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTF
AIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKG
KLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYN
SLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDL
EHLSKFIEEHATKLSRTKEEL"
BASE COUNT 618 a 570 c 632 g 530 t
ORIGIN
1 agaccccgag ttctcggtgc gctcagcggc cgccgacgct aggaggccgc gctccgcccc
61 cgctaccatg aggccccgga aagccttcct gctcctgctg ctcttggggc tggtgcagct
121 gctggccgtg gcgggtgccg agggcccgga cgaggattct tctaacagag aaaatgccat
181 tgaggatgaa gaggaggagg aggaggaaga tgatgatgag gaagaagacg acttggaagt
241 taaggaagaa aatggagtct tggtcctaaa tgatgcaaac tttgataatt ttgtggctga
301 caaagacaca gtgctgctgg agttttatgc tccatggtgt ggacattgca agcagtttgc
361 tccggaatat ggaaaaattg ccaacatatt aaaggataaa gatcctccca ttcctgttgc
421 caagatcgat gcaacctcag cgtctgtgct ggccagcagg tttgatgtga gtggctaccc
481 caccatcaag atccttaaga aggggcaggc tgtagactac gagggctcca gaacccagga
541 agaaattgtt gccaaggtca gagaagtctc ccagcccgac tggacgcctc caccagaagt
601 cacgcttgtg ttgaccaaag agaactttga tgaagttgtg aatgatgcag atatcattct
661 ggtggagttt tatgccccat ggtgtggaca ctgcaagaaa cttgcccccg agtatgagaa
721 ggccgccaag gagctcagca agcgttctcc tccaattccc ctggcaaagg tcgacgccac
781 cgcagaaaca gacctggcca agaggtttga tgtctctggc tatcccaccc tgaaaatttt
841 ccgcaaagga aggccttatg actacaacgg cccacgagaa aaatatggaa tcgttgatta
901 catgatcgag cagtccgggc ctccctccaa ggagattctg accctgaagc aggtccagga
961 gttcctgaag gatggagacg atgtcatcat catcggggtc tttaaggggg agagtgaccc
1021 agcctaccag caataccagg atgccgctaa caacctgaga gaagattaca aatttcacca
1081 cactttcagc acagaaatag caaagttctt gaaagtctcc caggggcagt tggttgtaat
1141 gcagcctgag aaattccagt ccaagtatga gccccggagc cacatgatgg acgtccaggg
1201 ctccacccag gactcggcca tcaaggactt cgtgctgaag tacgccctgc ccctggttgg
1261 ccaccgcaag gtgtcaaacg atgctaagcg ctacaccagg cgccccctgg tggtcgtcta
1321 ctacagtgtg gacttcagct ttgattacag agctgcaact cagttttggc ggagcaaagt
1381 cctagaggtg gccaaggact tccctgagta cacctttgcc attgcggacg aagaggacta
1441 tgctggggag gtgaaggacc tggggctcag cgagagtggg gaggatgtca atgccgccat
1501 cctggacgag agtgggaaga agttcgccat ggagccagag gagtttgact ctgacaccct
1561 ccgcgagttt gtcactgctt tcaaaaaagg aaaactgaag ccagtcatca aatcccagcc
1621 agtgcccaag aacaacaagg gacccgtcaa ggtcgtggtg ggaaagacct ttgactccat
1681 tgtgatggac cccaagaagg acgtcctcat cgagttctac gcaccatggt gcgggcactg
1741 caagcagcta gagcccgtgt acaacagcct ggccaagaag tacaagggcc aaaagggcct
1801 ggtcatcgcc aagatggacg ccactgccaa cgacgtcccc agcgaccgct ataaggtgga
1861 gggcttcccc accatctact tcgcccccag tggggacaaa aagaacccag ttaaatttga
1921 gggtggagac agagatctgg agcatttgag caagtttata gaagaacatg ccacaaaact
1981 gagcaggacc aaggaagagc tttgaaggcc tgaggtctgc ggaaggtggg aggaggcaga
2041 caccctgcgt ggcccatggt cggggcgtcc acgccgaggc cggcaacaaa cgacagtatc
2101 tcggattcct tttttttttt tttaattttt tatactttgg tgtttcactt catgctctga
2161 atactgaata accatgaatg actgaatagt ttagtccaga tttttacaga ggatacatct
2221 atttttatca ttatttgggg tttgaagaat ttttttttac accttctaat ttctttattt
2281 ctcaaagcag ataattcttc tgtgtgaaaa tgttttcttt ttttaattta aggtttaaaa
2341 ttcctttgcc
//