LOCUS AK290938 1755 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77754 complete cds, highly similar to Homo
sapiens keratin 8 (KRT8), mRNA.
ACCESSION AK290938
VERSION AK290938.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1755)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1755
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2NE2000068"
/clone_lib="NT2NE2"
/db_xref="H-InvDB:HIT000423735"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuron after the differentiation of
NT2 neuronal precursor cells."
/organism="Homo sapiens"
CDS 157..1608
/codon_start=1
/note="highly similar to Homo sapiens keratin 8 (KRT8),
mRNA"
/protein_id="BAF83627.1"
/transl_table=1
/translation="MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSS
NFRGGLGGGYGVASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKF
ASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLK
LEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLT
DEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRA
EAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAA
IADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYR
KLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSS
SFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK"
BASE COUNT 395 a 515 c 544 g 301 t
ORIGIN
1 gtaaagctct caaccctgtc cccttccccc tctctcctgg gaaagagccc tcccatgcct
61 agctgctgct cttagggacc ctgtggctag gtgcgcggat ggaaatccag gatctccgcc
121 tggttcggcc cgcctgcctc cactcctgcc tccaccatgt ccatcagggt gacccagaag
181 tcctacaagg tgtccacctc tggcccccgg gccttcagca gccgctccta cacgagtggg
241 cccggttccc gcatcagctc ctcgagcttc tcccgagtgg gcagcagcaa ctttcgcggt
301 ggcctgggcg gcggctatgg tgtggccagc ggcatgggag gcatcaccgc agttacggtc
361 aaccagagcc tgctgagccc ccttgtcctg gaggtggacc ccaacatcca ggccgtgcgc
421 acccaggaga aggagcagat caagaccctc aacaacaagt ttgcctcctt catagacaag
481 gtacggttcc tggagcagca gaacaagatg ctggagacca agtggagcct cctgcagcag
541 cagaagacgg ctcgaagcaa catggacaac atgttcgaga gctacatcaa caaccttagg
601 cggcagctgg agactctggg ccaggagaag ctgaagctgg aggcggagct tggcaacatg
661 caggggctgg tggaggactt caagaacaag tatgaggatg agatcaataa gcgtacagag
721 atggagaacg aatttgtcct catcaagaag gatgtggatg aagcttacat gaacaaggta
781 gagctggagt ctcgcctgga agggctgacc gacgagatca acttcctcag gcagctgtat
841 gaagaggaga tccgggagct gcagtcccag atctcggaca catctgtggt gctgtccatg
901 gacaacagcc gctccctgga catggacagc atcattgctg aggtcaaggc acagtacgag
961 gatattgcca accgcagccg ggctgaggct gagagcatgt accagatcaa gtatgaggag
1021 ctgcagagcc tggctgggaa gcacggggat gacctgcggc gcacaaagac tgagatctct
1081 gagatgaacc ggaacatcag ccggctccag gctgagattg agggcctcaa aggccagagg
1141 gcttccctgg aggccgccat tgcagatgcc gagcagcgtg gagagctggc cattaaggat
1201 gccaacgcca agttgtccga gctggaggcc gccctgcagc gggccaagca ggacatggcg
1261 cggcagctgc gtgagtacca ggagctgatg aacgtcaagc tggccctgga catcgagatc
1321 gccacctaca ggaagctgct ggagggcgag gagagccggc tggagtctgg gatgcagaac
1381 atgagtattc atacgaagac caccagcggc tatgcaggtg gtctgagctc ggcctatggg
1441 ggcctcacaa gccccggcct cagctacagc ctgggctcca gctttggctc tggcgcgggc
1501 tccagctcct tcagccgcac cagctcctcc agggccgtgg ttgtgaagaa gatcgagaca
1561 cgtgatggga agctggtgtc tgagtcctct gacgtcctgc ccaagtgaac agctgcggca
1621 gcccctccca gcctacccct cctgcgctgc cccagagcct gggaaggagg ccgctatgca
1681 gggtagcact gggaacagga gacccacctg aggctcagcc ctagccctca gcccacctgg
1741 ggagtttact acctg
//