LOCUS AK290932 1743 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ78528 complete cds, highly similar to Homo
sapiens vacuolar protein sorting 4B (yeast) (VPS4B), mRNA.
ACCESSION AK290932
VERSION AK290932.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1743)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1743
/cell_type="neutrophils"
/clone="NETRP2008966"
/clone_lib="NETRP2"
/db_xref="H-InvDB:HIT000423729"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, neutrophils"
/organism="Homo sapiens"
CDS 205..1539
/codon_start=1
/note="highly similar to Homo sapiens vacuolar protein
sorting 4B (yeast) (VPS4B), mRNA"
/protein_id="BAF83621.1"
/transl_table=1
/translation="MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHV
VKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDS
DGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLF
TGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEELVKN
LFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLG
ATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKAD
GYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAI
EMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG"
BASE COUNT 527 a 359 c 410 g 447 t
ORIGIN
1 attgcggaag tttggtgggg agggtcggag ctctggtgga gagagtgttg tctaaaacaa
61 gttccggaag ggaggctgcc cttcgcggtc cgagaaccac cggcctcccc agtttgaggg
121 ctgttacccc gtgcgcgctt cgacgttgct gctgttggct ctcctcgccc ctcgttccct
181 cgggaaccgc ctgggaactc cgccatgtca tccacttcgc ccaacctcca gaaagcgata
241 gatctggcta gcaaagcagc gcaagaagac aaggctggga actacgaaga agcccttcag
301 ctctatcagc atgctgtgca gtattttctt catgtcgtta aatatgaagc acagggtgat
361 aaagccaagc aaagtatcag ggcaaagtgt acagaatatc ttgatagagc agaaaaacta
421 aaggagtacc tgaaaaataa agagaaaaaa gcacagaagc cagtgaaaga aggacagccg
481 agtccagcag atgagaaggg gaatgacagt gatggggaag gagaatctga tgatcctgaa
541 aaaaagaaac tacagaatca acttcaaggt gccattgtta tagaacgacc aaatgtgaaa
601 tggagtgacg ttgctggact tgaaggagcc aaagaagcac tgaaagaggc tgtgatactg
661 cctattaaat ttcctcatct ttttacaggc aagagaacac cttggagggg aatcttatta
721 tttgggccgc ctggaacagg aaagtcctac ttagccaaag ctgtagcaac agaagccaac
781 aactcaacat ttttttcaat atcttcctct gatcttgttt ctaagtggct aggtgaaagt
841 gaagaactgg ttaagaattt attccaactt gccagagaga acaagccctc cattatcttc
901 attgatgaaa ttgattctct ctgtggttca agaagtgaaa atgaaagtga agccgcacgt
961 agaattaaga cggagttcct agtgcaaatg caaggggttg gtgtagacaa tgatggaatt
1021 ttggttctgg gagctacaaa tataccctgg gttctggatt ctgccattag gcgaagattt
1081 gagaaacgaa tttatattcc cttgccggaa ccccatgccc gagcagcaat gtttaaactg
1141 cacctaggga ccactcagaa cagtctcacg gaagcagact ttcgggaact tgggaggaaa
1201 gcagatggtt attcaggggc agatataagt atcattgtac gtgatgccct tatgcagcct
1261 gttaggaaag tacagtcagc tactcatttt aaaaaggttc gcggaccttc ccgagctgat
1321 cctaaccatc ttgtagatga tctgctaaca ccttgctctc caggtgaccc tggtgccatt
1381 gaaatgacat ggatggatgt ccctggagat aaacttttgg agccagttgt ttccatgtcg
1441 gatatgttgc ggtcactatc taacacaaaa cctacagtca atgaacatga cttgttgaaa
1501 ttaaagaagt ttacagaaga ttttggtcaa gaaggctaag ccaaagacaa ggaagatgct
1561 taccatatgt attctttctt tcatagatat ttttgtctat ttggatcgca ttaattgttt
1621 ccagtaaaac tcttttacca cagggaaata cacatctcac ttcagagttc cattaggttt
1681 tatattgtac ttttcctcca ttacttatta aatactccta ttaacaaaag gtacaaaata
1741 aca
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