LOCUS AK290894 3586 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76160 complete cds, highly similar to Homo
sapiens helicase (DNA) B (HELB), mRNA.
ACCESSION AK290894
VERSION AK290894.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3586)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3586
/cell_type="embryonal carcinoma"
/clone="NCRRM1000093"
/clone_lib="NCRRM1"
/db_xref="H-InvDB:HIT000423691"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced embryonal carcinoma."
/organism="Homo sapiens"
CDS 25..3288
/codon_start=1
/note="highly similar to Homo sapiens helicase (DNA) B
(HELB), mRNA"
/protein_id="BAF83583.1"
/transl_table=1
/translation="MARSSPYLRQLQGPLLPPRDLVEEDDDYLNDDVEEDEESVFIDA
EELCSGGVKAGSLPGCLRVSICDENTQETCKVFGRFPITGAWWRVKVQVKPVVGSRSY
QYQVQGFPSYFLQSDMSPPNQKHICALFLKECEVSSDDVNKFLTWVKEVSNYKNLNFE
NLRETLRTFHKETGRKDQKQPTQNGQEELFLDNEMSLPLENTIPFRNVMTALQFPKIM
EFLPVLLPRHFKWIIGSGSKEMLKEIEEILGTHPWKLGFSKITYREWKLLRCEASWIA
FCQCESLLQLMTDLEKNALIMYSRLKQICREDGHTYVEVNDLTLTLSNHMSFHAASES
LKFLKDIGVVTYEKSCVFPYDLYHAERAIAFPICDLMKKPPWHLCVDVEKVLASIHTT
KPENSSDDALNESKPDEVRLENPVDVVDTQDNGDHIWTNGENEINAEISEVQLDQDQV
EVPLDRDQVAALEMICSNPVTVISGKGGCGKTTIVSRLFKHIEQLEEREVKKACEDFE
QDQNASEEWITFTEQSQLEADKAIEVLLTAPTGKAAGLLRQKTGLHAYTLCQVNYSFY
SWTQTMMTTNKPWKFSSVRVLVVDEGSLVSVGIFKSVLNLLCEHSKLSKLIILGDIRQ
LPSIEPGNLLKDLFETLKSRNCAIELKTNHRAESQLIVDNATRISRRQFPKFDAELNI
SDNPTLPISIQDKTFIFVRLPEEDASSQSSKTNHHSCLYSAVKTLLQENNLQNAKTSQ
FIAFRRQDCDLINDCCCKHYTGHLTKDHQSRLVFGIGDKICCTRNAYLSDLLPENISG
SQQNNDLDASSEDFSGTLPDFAKNKRDFESNVRLCNGEIFFITNDVTDVTSGKRRSLT
INNMAGLEVTVDFKKLMKYCRIKHAWARTIHTFQGSEEQTVVYVVGKAGRQHWQHVYT
AVTRGRCRVYVIAEESQLRNAIMKNSFPRKTRLKHFLQSKLSSSGAPPADFLSPRKSS
GDSGGPSIPSASPLPVVTDHAMTNDVTWSEASSPDERTLTFAERWQLSSPDGVDTDDD
LPKSRASKRTCGVNDDESPSKIFMVGESPQVSSRLQNLRLNNLIPRQLFKPTDNQET"
BASE COUNT 1104 a 690 c 804 g 988 t
ORIGIN
1 gtttgggttg agttcaggag aagcatggcc aggtcgagtc cgtacctgcg ccaacttcag
61 ggacctctgc tcccacccag ggatctggtg gaggaggacg acgactacct aaacgacgac
121 gtggaggagg atgaagagtc cgtgttcatc gacgccgagg agctctgcag tgggggcgta
181 aaggctggca gcctccccgg gtgcctccgc gtttctattt gtgatgaaaa cacacaagag
241 acatgtaaag tgtttggacg ttttccgata acaggtgctt ggtggagagt gaaggtacaa
301 gtaaagcctg tggtgggatc aaggagctat caatatcaag ttcaaggatt tccgtcttac
361 tttttgcagt ctgatatgtc accaccaaat caaaaacata tctgtgctct ctttcttaaa
421 gagtgtgagg tctccagtga tgatgttaat aaatttttaa catgggtaaa ggaggtatca
481 aactacaaaa acctaaactt tgaaaatctt agggaaacac taagaacttt ccacaaggaa
541 actggaagga aagatcaaaa gcagcctaca cagaatggtc aggaagagtt gttcctagac
601 aatgagatga gtcttcctct ggaaaacaca attccattta gaaatgtaat gacagctttg
661 cagtttccga agataatgga attccttcca gttcttctgc ctcgacactt taaatggatc
721 atagggtcag gttctaaaga gatgttgaaa gagatagaag agattttagg tacacatccg
781 tggaaacttg gatttagtaa aataacctac agagagtgga aactcctgcg atgtgaggca
841 agttggatag cattttgtca gtgtgagtct cttctccagc tgatgactga tttggagaag
901 aatgcattaa taatgtattc cagactgaag cagatatgta gagaagatgg gcacacatat
961 gttgaagtga atgacttaac tttgacattg tcaaatcata tgtcatttca tgctgcttca
1021 gagtctctga agtttttgaa ggatattggt gtggtgacat atgagaagtc ctgtgtcttc
1081 ccttatgacc tttaccatgc tgaaagagcc atcgcctttc caatttgtga cctgatgaag
1141 aaacctcctt ggcatttatg tgtcgatgtc gaaaaggtgc ttgcctctat tcacaccaca
1201 aaacctgaga attcaagcga tgatgcattg aatgagagca aacctgatga agtaagatta
1261 gaaaatcctg tggatgttgt ggacacacag gacaatggtg accatatttg gactaatggt
1321 gaaaatgaaa ttaatgcaga aataagtgaa gttcagctgg atcaggatca ggttgaagtt
1381 ccactggatc gggatcaggt ggctgctttg gaaatgattt gctccaatcc tgtgacagtc
1441 ataagtggga aaggtggatg tgggaagacc acaatcgtta gccgtctttt taagcatata
1501 gagcagttgg aagaaagaga agtaaaaaaa gcctgtgaag attttgaaca agaccagaat
1561 gcttcagaag aatggattac ctttactgag caaagtcaac tagaggcgga caaggctata
1621 gaagttttgc tcacagcacc tacagggaaa gcagctggct tactaagaca gaaaactggt
1681 cttcatgcct acacactgtg tcaggtcaat tatagcttct attcatggac tcaaacaatg
1741 atgaccacaa acaaaccatg gaaattttct tcggttagag ttctggttgt ggatgaaggg
1801 agtttggtat ctgtaggaat cttcaaatcg gtcttaaatt tattgtgtga gcactccaaa
1861 ctttctaagc ttattatcct tggtgacatt agacagttac ccagtattga acctggtaac
1921 ttgctgaaag atctttttga gactcttaag tcaagaaatt gtgctattga gctaaagaca
1981 aaccatagag cagaatctca gctcattgtg gacaatgcta caagaatctc aagacgccaa
2041 tttccaaaat ttgatgcaga actaaatatc tctgataatc caacattacc catctcaatt
2101 caagataaga catttatttt tgtcaggctc ccagaagagg atgccagttc tcagtcatct
2161 aaaactaatc atcactcttg tttatattct gcagttaaaa ctttactaca agaaaataac
2221 ttacaaaatg caaaaacatc acaatttatt gcatttagaa ggcaagactg tgatctaatt
2281 aatgactgct gctgcaaaca ctacacaggc cacctcacca aagaccatca gagtagactt
2341 gtttttggaa ttggtgataa aatttgttgt accaggaatg catacctctc agacttacta
2401 cctgaaaata tctctggaag tcagcaaaat aatgatctag atgccagtag tgaagacttt
2461 tctggtacgc ttcctgattt tgctaaaaat aagcgtgact ttgaaagtaa cgttcgactg
2521 tgcaatggag agatattttt cataacaaat gatgtaactg atgtaacttc tggaaagaga
2581 agatctttga ccattaataa tatggctggc ctggaagtaa ctgtggattt taagaaacta
2641 atgaaatatt gtcgcataaa acatgcatgg gcaagaacta ttcacacttt tcaggggtcc
2701 gaggagcaaa cagttgtcta tgtggtgggg aaggcgggcc gccagcactg gcagcatgtc
2761 tacaccgccg tgaccagggg ccgctgccga gtgtatgtga ttgcagagga gtctcagctc
2821 cggaatgcca ttatgaaaaa cagttttcct agaaaaactc gtttgaaaca tttcttgcaa
2881 agtaagctct cctctagcgg cgcacctcca gcagattttc tgtccccacg gaagagctct
2941 ggagacagtg gaggacccag cataccgtca gcatctccac tccctgtagt cacagaccac
3001 gccatgacaa atgatgtcac ctggagcgag gcctcttcgc ctgatgagag gacactcacc
3061 tttgctgaaa gatggcaatt atcttcacct gatggagtag atacagatga tgatttacca
3121 aaatcgcgag catccaaaag aacctgtggt gtgaatgatg atgaaagtcc aagcaaaatt
3181 tttatggtgg gagaatctcc acaagtgtct tccagacttc agaatttgag actgaataat
3241 ttaattccca ggcaactttt caagcccaca gataatcaag aaacttagtt ttatttcaaa
3301 ttgttccgag taactatgtt tttctattgg agacaaaatg aacatcgtaa cgtcaaagta
3361 ccaagataaa aaaagtttcc tataactgga gttttaagag ataggggttc accatggtgc
3421 ccaggctggt ctcgaactcc tggactcagg cagtctgccc acctcgacct cccaaagtgc
3481 tgggattaca ggtgtgagcc atcatgcttg gttcttcttt aattctttgt agttttcaga
3541 gtataagttt tgtatttctt ttattaaatt tacaatgctc tttgtt
//