LOCUS AK290873 2371 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76156 complete cds, highly similar to Homo
sapiens aspartyl-tRNA synthetase 2 (DARS2), mRNA.
ACCESSION AK290873
VERSION AK290873.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2371)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2371
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2005086"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000423670"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 254..2191
/codon_start=1
/note="highly similar to Homo sapiens aspartyl-tRNA
synthetase 2 (DARS2), mRNA"
/protein_id="BAF83562.1"
/transl_table=1
/translation="MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEF
SSFVVRTNTCGELRSSHLGQEVTLCGWIQCRRQNTFLVLRDFDGLVQVIIPQDESAAS
VKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIEIKVKTAELLNACKKLPFEIK
NFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKR
TPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQP
EFTQIDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPD
TRFEMKIIDISDVFRNTEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAA
DHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTAGEHNKACS
LLGKLRLECADLLETRGVVLRDPTLFSFLWVVDFPLFLPKEENPRELESAHHPFTAPH
PSDIHLLYTEPKKARSQHYDLVLNGNEIGGGSIRIHNAELQRYILATLLKEDVKMLSH
LLQALDYGAPPHGGIALGLDRLICLVTGSPSIRDVIAFPKSFRGHDLMSNTPDSVPPE
ELKPYHIRVSKPTDSKAERAH"
BASE COUNT 665 a 534 c 537 g 635 t
ORIGIN
1 agactgatgc tttaagactc agggagaggt ctttccctta tctccacccc agcaagcacc
61 ccagagacct tggagatttg tcttgtttct agacacgtgt actccaatgt tgtgcggagg
121 aggccttaaa tattcgagaa gagagtggga actcctggaa ttttaaggga tttctgtgta
181 tttccaaaac tgacttttaa ctaccggcag cgtgggattt cgtgattgtt tttcgccatc
241 gtgtggctcc aacatgtact tcccttcttg gttaagtcag ctgtacaggg gtttatccag
301 acccatcaga aggaccaccc aaccgatctg gggttctctc tacagaagtc tgttgcagag
361 ttcacagagg agaattccag aattcagtag ctttgttgtc cggaccaaca catgtggaga
421 gttgcgttcg tctcacttag gccaagaagt caccttgtgt ggatggattc agtgccgaag
481 gcaaaacaca ttcttggtcc taagagattt cgatgggctt gttcaagtta tcattcccca
541 ggatgagtcg gcagcctctg tgaagaagat tttatgtgaa gcccctgtgg aatctgtggt
601 gcaagtgtct ggtacagtca tttcccgtcc tgcaggacaa gagaatccaa aaatgccaac
661 aggtgagatt gaaatcaaag ttaaaacagc tgagcttctg aatgcctgca agaagctgcc
721 ctttgaaatt aagaacttcg tgaagaaaac agaggctctt cggttgcagt atcgctactt
781 agacttgcgt agtttccaaa tgcagtataa cctgcgactg aggtcccaga tggtcatgaa
841 aatgcgggaa tatctctgta atctgcatgg gtttgtggat atagaaaccc ccacattgtt
901 taagaggacc ccagggggtg ccaaagagtt tttagtacca tccagggaac ctggaaagtt
961 ttattctctc cctcagagtc ctcaacagtt taagcaactt ctgatggttg gcggtttaga
1021 cagatatttt caggttgccc gatgttatcg agatgaaggt tcaagaccag acagacagcc
1081 tgagtttact cagattgaca tagagatgtc atttgtagac cagactggga tccagagttt
1141 aattgagggt ttgctccagt attcctggcc caatgacaaa gatcctgtgg ttgttccctt
1201 tcctactatg acttttgctg aggtgctggc cacctatgga actgataaac ctgacactcg
1261 ctttgaaatg aagattatag atatcagtga tgtgtttaga aacacagaga ttggatttct
1321 tcaagatgca cttagtaagc cccatggaac tgtgaaagcc atatgtatcc ctgaaggagc
1381 aaaatactta aaaaggaaag acattgaatc cattagaaac tttgcagctg accattttaa
1441 tcaggaaatc ttacctgtat tccttaacgc caatagaaac tggaattctc cagttgctaa
1501 tttcataatg gagtcacaaa gactggaatt aatcagacta atggagaccc aagaggaaga
1561 tgtggtccta ctaactgctg gagagcacaa taaagcatgc tctttgttag gaaaattacg
1621 actggaatgt gctgaccttc tagaaacaag aggagtggtg ctccgtgacc ccactctgtt
1681 ctctttcctt tgggtggtag atttcccact cttcctgccc aaggaggaaa atcccagaga
1741 gctggaatcg gcccaccacc catttactgc tccccacccc agtgacatac atctcctgta
1801 cactgagccc aaaaaggccc gtagccaaca ctatgacttg gttttaaatg gcaatgaaat
1861 aggaggtggt tcaattcgaa ttcacaatgc agagctgcag cgttatatcc tggcaacctt
1921 actaaaggag gatgtgaaaa tgctctccca tctgctccag gctttagatt atggggcacc
1981 ccctcatgga ggaattgcct tagggttaga cagactgata tgccttgtca ctggatctcc
2041 aagcatcaga gatgtcatag ccttcccaaa gtccttccgg ggacatgacc tcatgagcaa
2101 taccccagat tctgtccctc ctgaggaact gaagccctat catatccgag tctccaagcc
2161 aacagactcc aaagcagaaa gagctcattg aatcatgcat accatgcaga aagttgagct
2221 tttaggtttt gtcctctttg cttccccaag gctaaagtca gatctagagt tctgccacag
2281 gtctaacaat caagtcttta gatggaagga atccaggcaa cattcttcac cacaacgaag
2341 aaacagataa aagataccca attttgactt g
//