LOCUS AK290723 2807 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77079 complete cds, highly similar to Homo
sapiens aggrecan 1 (chondroitin sulfate proteoglycan 1, large
aggregating proteoglycan, antigen identified by monoclonal
antibody A0122), mRNA.
ACCESSION AK290723
VERSION AK290723.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2807)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2807
/cell_type="synoviocytes from rheumatioid arthritis
(HS-RA)"
/clone="HSYRA1000192"
/clone_lib="HSYRA1"
/db_xref="H-InvDB:HIT000423520"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, synoviocytes from rheumatioid
arthritis"
/organism="Homo sapiens"
CDS 375..2540
/codon_start=1
/note="highly similar to Homo sapiens aggrecan 1
(chondroitin sulfate proteoglycan 1, large aggregating
proteoglycan, antigen identified by monoclonal antibody
A0122), mRNA"
/protein_id="BAF83412.1"
/transl_table=1
/translation="MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLG
TSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQD
KVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAI
STRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDDFHQCDAGWLADQTVRYPIHTPRE
GCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRL
GARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQ
TGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGE
ARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGL
GPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIA
SPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTET
YDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYA
GWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGMQPHFG
SNSPFCLERTPLGSPDPATTQYPIHQSKKMSVPLGQSQL"
BASE COUNT 577 a 912 c 801 g 517 t
ORIGIN
1 cacctacctc cccgccgctc cagagggggc tcgcagagct gaggacgcgc gcagcgctgc
61 tcaaggtctc tctctctcag caccctcgcc ggccggcgtc tgacgcgggt gccagggtct
121 ccgggcacct ttcagtgtcc attccctcag ccagccagga ctccgcaacc cagcagttgc
181 cgctgcggcc acagcccgag gggacctgcg gacaggacgc cggcaggaga aggggtgcgc
241 agcgcccgcg cagagcgtct ccctcgctac gcagcgagac ccgggcctcc cggccccagg
301 agcccccagc tgcctcgcca ggtgtgtggg actgaagttc ttggagaagg gagtccaact
361 cttcaaggtg aactatgacc actttactct gggttttcgt gactctgagg gtcatcactg
421 cagctgtcac tgtagaaact tcagaccatg acaactcgct gagtgtcagc atcccccaac
481 cgtccccgct gagggtcctc ctggggacct ccctcaccat cccctgctat ttcatcgacc
541 ccatgcaccc tgtgaccacc gccccttcta ccgccccact ggccccaaga atcaagtgga
601 gccgtgtgtc caaggagaag gaggtagtgc tgctggtggc cactgaaggg cgcgtgcggg
661 tcaacagtgc ctatcaggac aaggtctcac tgcccaacta cccggccatc cccagtgacg
721 ccaccttgga agtccagagc ctgcgctcca atgactctgg ggtctaccgc tgcgaggtga
781 tgcatggcat cgaggacagc gaggccaccc tggaagtcgt ggtgaaaggc atcgtgttcc
841 attacagagc catctctaca cgctacaccc tcgactttga cagggcgcag cgggcctgcc
901 tgcagaacag tgccatcatt gccacgcctg agcagctgca ggccgcctac gaagacgact
961 tccaccagtg tgacgccggc tggctggctg accagactgt cagatacccc atccacactc
1021 cccgggaagg ctgctatgga gacaaggatg agtttcctgg tgtgaggacg tatggcatcc
1081 gagacaccaa cgagacctat gatgtgtact gcttcgccga ggagatggag ggtgaggtct
1141 tttatgcaac gtctccagag aagttcacct tccaggaagc agccaatgag tgccggcggc
1201 tgggtgcccg gctggccacc acgggccagc tctacctggc ctggcaggct ggcatggaca
1261 tgtgcagcgc cggctggctg gccgaccgca gcgtgcgcta ccccatctcc aaggcccggc
1321 ccaactgcgg tggcaacctc ctgggcgtga ggaccgtcta cgtgcatgcc aaccagacgg
1381 gctaccccga cccctcatcc cgctacgacg ccatctgcta cacaggtgaa gactttgtgg
1441 acatcccaga aaacttcttt ggagtggggg gtgaggagga catcaccgtc cagacagtga
1501 cctggcctga catggagctg ccactgcctc gaaacatcac tgagggtgaa gcccgaggca
1561 gcgtgatcct taccgtaaag cccatcttcg aggtctcccc cagtcccctg gaacccgagg
1621 agcccttcac gtttgcccct gaaatagggg ccactgcctt cgctgaggtt gagaatgaga
1681 ctggagaggc caccaggccc tggggctttc ccacacctgg cctgggccct gccacggcat
1741 tcaccagtga ggacctcgtc gtgcaggtga ccgctgtccc tgggcagccg catttgccag
1801 ggggggtcgt cttccactac cgcccgggac ccacccgcta ctcgctgacc tttgaggagg
1861 cacagcaggc ctgcctgcgc acgggggcgg tcattgcctc gccggagcag ctccaggccg
1921 cctacgaagc aggctatgag cagtgtgacg ccggctggct gcgggaccag accgtcagat
1981 accccattgt gagcccccgg accccatgcg tgggtgacaa ggacagcagc ccaggggtca
2041 ggacctatgg cgtgcgccca tcaacagaga cctacgatgt ctactgcttt gtagacagac
2101 ttgaggggga ggtgttcttc gccacacgcc ttgagcagtt caccttccag gaagcactgg
2161 agttctgtga atctcacaat gccacgctgg ccaccacggg ccagctctac gccgcctgga
2221 gccgcggcct ggacaagtgc tatgccggct ggctggccga cggcagcctc cgctacccca
2281 tcgtcacccc aaggcctgcc tgcggtgggg acaagccagg cgtgagaacg gtctacctct
2341 accctaacca gacgggcctc ccagacccac tgtcccggca ccatgccttc tgcttccgag
2401 gtatgcagcc tcacttcggc tccaacagcc ccttttgtct ggagaggacc ccactgggtt
2461 caccggatcc tgccaccacc cagtatccca tccatcagag caagaaaatg tcagtccctc
2521 tggggcagag ccagctctga aaccagcaca acgcaggctt tgaccccaag gcaaggtcat
2581 ccttctaaag ttccccagag acaagtagag ataaataaga acttgagctg gtatttatgt
2641 ctactagaaa tgaagcagac ctgaatttga gttatggctc tgctacttaa tagctgtgtg
2701 aaaatggaga tataatggtt cctacttcag agagagctgc aagaattaat taagtgcacg
2761 tggtgccctt ggcatagtgc ctgggacaca gtaaatgctc aataaat
//