LOCUS AK290578 1735 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77655 complete cds.
ACCESSION AK290578
VERSION AK290578.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1735)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1735
/cell_type="coronary artery smooth muscle cells (HCASMC)"
/clone="HCASM2005953"
/clone_lib="HCASM2"
/db_xref="H-InvDB:HIT000423375"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, coronary artery smooth muscle
cells"
/organism="Homo sapiens"
CDS 209..1690
/codon_start=1
/protein_id="BAF83267.1"
/transl_table=1
/translation="MILNSLSLCYHNKLILAPMVRVGTLPMRLLALDYGADIVYCEEL
IDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGTSDAERALAVARL
VENDVAGIDVNMGCPKQYSTKGGMGAALLSDPDKIEKILSTLVKGTRRPVTCKIRILP
SLEDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIKAIADTLSIPVIANGGSH
DHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGLRPLEEVMQKYIRYAVQYDN
HYTNTKYCLCQMLREQLESPQGRLLHAAQSSREICEAFGLGAFYEETTQELDAQQARL
SAKTSEQTGEPAEDTSGVIKMAVKFDRRAYPAQITPKMCLLEWCRREKLAQPVYETVQ
RPLDRLFSSIVTVAEQKYQSTLWDKSKKLAEQAAAIVCLRSQGLPEGRLGEESPSLHK
RKREAPDQDPGGPRAQELAQPGDLCKKPFVALGSGEESPLEGW"
BASE COUNT 431 a 438 c 495 g 371 t
ORIGIN
1 acggacgcgc cgcgacctcg cgtacggtgg ctggcgaggc tcagtacggt gtgtggagct
61 ggagcaccgt gaggaagaag cgaggttctt tttaagagtt cagctgcgag atatcaaaca
121 aagaattact ctgtacaaag ccagaacaca tatatcaaag taatcctgaa gtatcagaac
181 aaaataatag gctgtaacag aggaggaaat gattttgaat agcctctctc tgtgttacca
241 taataagcta atcctggccc caatggttcg ggtagggact cttccaatga ggctgctggc
301 cctggattat ggagcggaca ttgtttactg tgaggagctg atcgacctca agatgattca
361 gtgcaagaga gttgttaatg aggtgctcag cacagtggac tttgtcgccc ctgatgatcg
421 agttgtcttc cgcacctgtg aaagagagca gaacagggtg gtcttccaga tggggacttc
481 ggacgcagag cgagcccttg ctgtggccag gcttgtagaa aatgatgtgg ctggtattga
541 tgtcaacatg ggctgtccaa aacaatattc caccaaggga ggaatgggag ctgccctgct
601 gtcagaccct gacaagattg agaagatcct cagcactctt gttaaaggga cacgcagacc
661 tgtgacctgc aagattcgca tcctgccatc gctagaagat accctgagcc ttgtgaagcg
721 gatagagagg actggcattg ctgccatcgc agttcatggg aggaagcggg aggagcgacc
781 tcagcatcct gtcagctgtg aagtcatcaa agccattgct gataccctct ccattcctgt
841 catagccaac ggaggatctc atgaccacat ccaacagtat tcggacatag aggactttcg
901 acaagccacg gcagcctctt ccgtgatggt ggcccgagca gccatgtgga acccatctat
961 cttcctcaag gagggtctgc ggcccctgga ggaggtcatg cagaaataca tcagatacgc
1021 ggtgcagtat gacaaccact acaccaacac caagtactgc ttgtgccaga tgctacgaga
1081 acagctggag tcgccccagg gaaggttgct ccatgctgcc cagtcttccc gggaaatttg
1141 tgaggccttt ggccttggtg ccttctatga ggagaccaca caggagctgg atgcccagca
1201 ggccaggctc tcagccaaga cttcagagca gacaggggag ccagctgaag atacctctgg
1261 tgtcattaag atggctgtca agtttgaccg gagagcatac ccagcccaga tcacccctaa
1321 gatgtgccta ctagagtggt gccggaggga gaagttggca cagcctgtgt atgaaacggt
1381 tcaacgccct ctagatcgcc tgttctcctc tattgtcacc gttgctgaac aaaagtatca
1441 gtctaccttg tgggacaagt ccaagaaact ggcggagcag gctgcagcca tcgtctgtct
1501 gcggagccag ggcctccctg agggtcggct gggtgaggag agcccttcct tgcacaagcg
1561 aaagagggag gctcctgacc aagaccctgg gggccccaga gctcaggagc tagcacaacc
1621 tggggatctg tgcaagaagc cctttgtggc cttgggaagt ggtgaagaaa gccccctgga
1681 aggctggtga ctactcttcc tgccttagtc acccctccat gggcctggtg ctaag
//