LOCUS AK290418 1893 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76781 complete cds, highly similar to Homo
sapiens cytochrome P450, family 27, subfamily A, polypeptide 1
(CYP27A1), mRNA.
ACCESSION AK290418
VERSION AK290418.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1893)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1893
/clone="D9OST2001975"
/clone_lib="D9OST2"
/db_xref="H-InvDB:HIT000423215"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from CD34+ cells after 9-days ODF induction."
/note="primary culture, CD34+ cells"
/organism="Homo sapiens"
/tissue_type="cord blood"
CDS 41..1636
/codon_start=1
/note="highly similar to Homo sapiens cytochrome P450,
family 27, subfamily A, polypeptide 1 (CYP27A1), mRNA"
/protein_id="BAF83107.1"
/transl_table=1
/translation="MAALGCARLRWALRGAGRGLCPHGARAKAAIPAALPSDKATGAP
GAGPGVRRRQRSLEEIPRLGQLRFFFQLFVQGYALQLHQLQVLYKAKYGPMWMSYLGP
QMHVNLASAPLLEQVMRQEGKYPVRNDMELWKEHRDQHDLTYGPFTTEGHHWYQLRQA
LNQRLLKPAEAALYTDAFNEVIDDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAIC
YILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWN
AIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLM
AGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKE
TLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWL
RNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGEL
KSVARIVLVPNKKVGLQFLQRQC"
BASE COUNT 393 a 553 c 550 g 397 t
ORIGIN
1 aaaaactcga cccaaaggtg caggcgcgcg agcacaaccc atggctgcgc tgggctgcgc
61 gaggctgagg tgggcgctgc gaggggccgg ccgtggcctc tgcccccacg gggccagagc
121 caaggccgcg atccctgccg ccctcccctc ggacaaggcc accggagctc ccggagccgg
181 gcctggtgtc cggcggcggc aacggagctt agaggagatt ccacgtctag gacagctgcg
241 cttcttcttt cagctgttcg ttcaaggcta tgccctgcaa ctgcaccagt tacaggtgct
301 ttacaaggcc aagtacggtc caatgtggat gtcctactta gggcctcaga tgcacgtgaa
361 cctggccagt gccccgctct tggagcaagt gatgcggcaa gagggcaagt acccagtacg
421 gaacgacatg gagctatgga aggagcaccg ggaccagcac gacctgacct atgggccgtt
481 caccacggaa ggacaccact ggtaccagct gcgccaggct ctgaaccagc ggttgctgaa
541 gccagcggaa gcagcgctct atacggatgc tttcaatgag gtgattgatg actttatgac
601 tcgactggac cagctgcggg cagagagtgc ttcggggaac caggtgtcgg acatggctca
661 actcttctac tactttgcct tggaagctat ttgctacatc ctgttcgaga aacgcattgg
721 ctgcctgcag cgatccatcc ccgaggacac cgtgaccttc gtcagatcca tcgggttaat
781 gttccagaac tcactctatg ccaccttcct ccccaagtgg actcgccccg tgctgccttt
841 ctggaagcga tacctggatg gttggaatgc catcttttcc tttgggaaga agctgattga
901 tgagaagctc gaagatatgg aggcccaact gcaggcagca gggccagatg gcatccaggt
961 gtctggctac ctgcacttct tactggccag tggacagctc agtcctcggg aggccatggg
1021 cagcctgcct gagctgctca tggctggagt ggacacgaca tccaacacgc tgacatgggc
1081 cctgtaccac ctctcaaagg accctgagat ccaggaggcc ttgcacgagg aagtggtggg
1141 tgtggtgcca gccgggcaag tgccccagca caaggacttt gcccacatgc cgttgctcaa
1201 agctgtgctt aaggagactc tgcgtctcta ccctgtggtc cccacaaact cccggatcat
1261 agaaaaggaa attgaagttg atggcttcct cttccccaag aacacccagt ttgtgttctg
1321 ccactatgtg gtgtcccggg accccactgc cttctctgag cctgaaagct tccagcccca
1381 ccgctggctg agaaacagcc agcctgctac ccccaggatc cagcacccat ttggctctgt
1441 gccctttggc tatggggtcc gggcctgcct gggccgcagg attgcagagc tggagatgca
1501 gctactcctc gcaaggctga tccagaagta caaggtggtc ctggccccgg agacggggga
1561 gttgaagagt gtggcccgca ttgtcctggt tcccaataag aaagtgggcc tgcagttcct
1621 gcagagacag tgctgagctg agtctccgcc ttgctggggc ttgtcctaga ggctccagct
1681 ctggcacagt ggttcctggc tgctgccatg tctcagatga ggagggagag aaggaggccg
1741 ccagactcga gaggtgggag gaactccttg cacacaccct gagcttttgc cacttctatc
1801 atttttgagc aactccctct cagctaaaag gccacccctt tatcgcattg ctgtccttgg
1861 gtaggatata aaataaaggg acttttattt ctt
//