LOCUS AK290063 1651 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ76590 complete cds, highly similar to Homo
sapiens NEDD8-conjugating enzyme (NCE2), mRNA.
ACCESSION AK290063
VERSION AK290063.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1651)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1651
/clone="BRSSN2001127"
/clone_lib="BRSSN2"
/db_xref="H-InvDB:HIT000422860"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="substantia nigra"
CDS 116..673
/codon_start=1
/note="highly similar to Homo sapiens NEDD8-conjugating
enzyme (NCE2), mRNA"
/protein_id="BAF82752.1"
/transl_table=1
/translation="MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAEL
EANLPCTCKVHFPDPNKLHCFQLTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLT
KIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIE
AAEHHLRDKEDFRNKVDDYIERYAR"
BASE COUNT 450 a 344 c 394 g 463 t
ORIGIN
1 gagccggtgc ggctgtgagg ggccgcgtct cgcagcagcc gcccggaccg ggcatggtgt
61 tgggcgccgg gcccgcctcg cctgtctcgg ggagcccagg gtaaaggcag cagtaatgct
121 aacgctagca agtaaactga agcgtgacga tggtctcaaa gggtcccgga cggcagccac
181 agcgtccgac tcgactcgga gggtttctgt gagagacaaa ttgcttgtta aagaggttgc
241 agaacttgaa gctaatttac cttgtacatg taaagtgcat tttcctgatc caaacaagct
301 tcattgtttt cagctaacag taaccccaga tgagggttac taccagggtg gaaaatttca
361 gtttgaaact gaagttcccg atgcgtacaa catggtgcct cccaaagtga aatgcctgac
421 caagatctgg caccccaaca tcacagagac aggggaaata tgtctgagtt tattgagaga
481 acattcaatt gatggcactg gctgggctcc cacaagaaca ttaaaggatg tcgtttgggg
541 attaaactct ttgtttactg atcttttgaa ttttgatgat ccactgaata ttgaagctgc
601 agaacatcat ttgcgggaca aggaggactt ccggaataaa gtggatgact acatcgaacg
661 ttatgccaga tgataaaagg ggacgattgc aggcccatgg actgtgttac agtttgtctc
721 taacatgaaa cagcaagagg tagccccctc tcccgtcctc atgctccctc tcagtcccct
781 ggattgcccc agtcctgtga ccatgttgcc ctgaagaaga ccatcttcat gactgctcat
841 tgtagatgga gaattcaaca taaatacagc aagaaaatgt gtttgggctt ctgaagagtt
901 gtctgcttac cttaacatgt ttactttttt gaacttgtac tgtataggct gttggtgaaa
961 ttcttaagaa gttgtaatga actcaaaatt gaggccagag cttgctttcc cttttcccaa
1021 acaaaattgg ttttctgcac aagcgatgct aatgatgtgt tcagtgtaac tcgcagattg
1081 gcaataagat acccgctaca aactgtgatt ggatgcaaaa tctcttagct tctttcacga
1141 atgttggccc tgcctagatg ttgtgaagcc tcccagaatg catagagtca ttcactgtag
1201 atctcttatt gaaatgcgta ttttatttaa tgtaagtata ttttggaaca gatttgtaat
1261 ttgtacaatt caatgcttta attatttttt ctattctcat ttagtttgta ttttcattgt
1321 atagagcaga cagaaagatg ttgggtcaag caactattga agagaaatac aaagaaaata
1381 tgaaaggcac attattcatt ttgtccaaat gcaatgagaa tctcactctt aaaaatcagc
1441 tcttgctttc gggtccggat gtggtgagca cattttggag ccctttgaag ctagatttgg
1501 atgatcaaaa caaaaaggca gggagcccat tctaacatgc tgcccagagg aaactggctg
1561 gagcctggac cagctggggc tgatgctttt gcagtggtca tgtgattgtg acctggtagc
1621 tacttatcag agagccagac cctgctgtcc t
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