LOCUS AK289432 1998 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ75536 partial cds, highly similar to Homo
sapiens cysteinyl-tRNA synthetase (CARS), transcript variant1,
mRNA.
ACCESSION AK289432
VERSION AK289432.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1998)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1998
/cell_line="GI1"
/cell_type="glioma separated from gliosarcoma"
/clone="BGGI12000774"
/clone_lib="BGGI12"
/db_xref="H-InvDB:HIT000422229"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 58..>1998
/codon_start=1
/note="ighly similar to Homo sapiens cysteinyl-tRNA
synthetase (CARS), transcript variant1, mRNA"
/protein_id="BAF82121.1"
/transl_table=1
/translation="MADSSGQQGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIP
QDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVFYCMNITDIDDKI
IKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQ
LATEPLEKAVQSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKF
WEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKFA
SSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWP
CPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVR
YFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKGTLDYSSNTME
SALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDN
VDTRTVMEEMRALVSQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSS
LGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPE
LGVRFEDHEGLPTVVKLVDRNTL"
BASE COUNT 506 a 508 c 550 g 434 t
ORIGIN
1 tgcatcagat tctaggaagt gtctgtagcc gcagctgcgg gtccgggatt cccagccatg
61 gcagattcct ccgggcagca gggcaaaggc cggcgtgtgc agccccagtg gtcccctcct
121 gctgggaccc agccatgcag actccacctt tacaacagcc tcaccaggaa caaggaagtg
181 ttcatacctc aagatgggaa aaaggtgacg tggtattgct gtgggccaac cgtctatgac
241 gcatctcaca tggggcacgc caggtcctac atctcttttg atatcttgag aagagtgttg
301 aaggattact tcaaatttga tgtcttttat tgcatgaaca ttacggatat tgatgacaag
361 atcatcaaga gggcccggca gaaccacctg ttcgagcagt atcgggagaa gaggcctgaa
421 gcggcacagc tcttggagga tgttcaggcc gccctgaagc cattttcagt aaaattaaat
481 gagaccacgg atcccgataa aaagcagatg ctcgaacgga ttcagcacgc agtgcagctt
541 gccacagagc cacttgagaa agctgtgcag tccagactca cgggagagga agtcaacagc
601 tgtgtggagg tgttgctgga agaagccaag gatttgctct ctgactggct ggattctaca
661 cttggctgtg atgtcactga caattccatc ttctccaagc tgcccaagtt ctgggagggg
721 gacttccaca gagacatgga agctctgaat gttctccctc cagatgtctt aacccgggtt
781 agtgagtatg tgccagaaat tgtgaacttt gtccagaaga ttgtggacaa cggttacggc
841 tatgtctcca atgggtctgt ctactttgat acagcgaagt ttgcttctag cgagaagcac
901 tcctatggga agctggtgcc tgaggccgtt ggagatcaga aagcccttca agaaggggaa
961 ggtgacctga gcatctctgc agaccgcctg agtgagaagc gctctcccaa cgactttgcc
1021 ttatggaagg cctctaagcc cggagaaccg tcctggccgt gcccttgggg aaagggtcgt
1081 ccgggctggc atatcgagtg ctcggccatg gcaggcaccc tcctaggggc ttcgatggac
1141 attcacggag gtgggttcga cctccggttc ccccaccatg acaatgagct ggcacagtcg
1201 gaggcctact ttgaaaacga ctgctgggtc aggtacttcc tgcacacagg ccacctgacc
1261 attgcaggct gcaaaatgtc aaagtcacta aaaaacttca tcaccattaa agatgccttg
1321 aaaaagcact cagcacggca gttgcggctg gccttcctca tgcactcgtg gaagggcacc
1381 ctggactact ccagcaacac catggagtca gcgcttcaat atgagaagtt cttgaatgag
1441 tttttcttaa atgtgaaaga tatccttcgc gctcctgttg acatcactgg tcagtttgag
1501 aagtggggag aagaagaagc agaactgaat aagaactttt atgacaagaa gacagcaatt
1561 cacaaagccc tctgtgacaa tgttgacacc cgcaccgtca tggaagagat gcgggccttg
1621 gtcagtcagt gcaacctcta tatggcagcc cggaaagccg tgaggaagag gcccaaccag
1681 gctctgctgg agaacatcgc cctgtacctc acccatatgc tgaagatctt tggggccgta
1741 gaagaggaca gctccctggg attcccggtc ggagggcctg gaaccagcct cagtctcgag
1801 gccacagtca tgccctacct tcaggtgtta tcagaattcc gagaaggagt gcggaagatt
1861 gcccgagagc aaaaagtccc tgagattctg cagctcagcg atgccctgcg ggacaacatc
1921 ctgcctgagc ttggggtgcg gtttgaagac cacgaaggac tgcccacagt ggtgaaactg
1981 gtagacagaa acacctta
//