LOCUS AK289396 2125 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77925 complete cds, highly similar to Homo
sapiens kinetochore associated 2, mRNA.
ACCESSION AK289396
VERSION AK289396.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2125)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2125
/cell_type="normal astrocytes (NHA5732)"
/clone="ASTRO2004508"
/clone_lib="ASTRO2"
/db_xref="H-InvDB:HIT000422193"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal astrocytes"
/organism="Homo sapiens"
CDS 137..2065
/codon_start=1
/note="highly similar to Homo sapiens kinetochore
associated 2, mRNA"
/protein_id="BAF82085.1"
/transl_table=1
/translation="MKRSSVSSGGAGRLSMQELRSQDVNKQGLYTPQTKEKPTFGKLS
INKPTSERKVSLFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEF
LTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGY
PFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDG
IMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALN
EQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEE
IARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQ
KLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCL
VKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQ
KLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQL
VVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIK
EIRDKYEKKATLIKSSEE"
BASE COUNT 756 a 363 c 479 g 527 t
ORIGIN
1 aaattcgaac ggctttggcg ggccgaggaa ggacctggtg ttttgatgac cgctgtcctg
61 tctagcagat acttgcacgg tttacagaaa ttcggtccct gggtcgtgtc aggaaactgg
121 aaaaaaggtc ataagcatga agcgcagttc agtttccagc ggtggtgctg gccgcctctc
181 catgcaggag ttaagatccc aggatgtaaa taaacaaggc ctctataccc ctcaaaccaa
241 agagaaacca acctttggaa agttgagtat aaacaaaccg acatctgaaa gaaaagtctc
301 gctatttggc aaaagaacta gtggacatgg atcccggaat agtcaacttg gtatattttc
361 cagttctgag aaaatcaagg acccgagacc acttaatgac aaagcattca ttcagcagtg
421 tattcgacaa ctctgtgagt ttcttacaga aaatggttat gcacataatg tgtccatgaa
481 atctctacaa gctccctctg ttaaagactt cctgaagatc ttcacatttc tttatggctt
541 cctgtgcccc tcatacgaac ttcctgacac aaagtttgaa gaagaggttc caagaatctt
601 taaagacctt gggtatcctt ttgcactatc caaaagctcc atgtacacag tgggggctcc
661 tcatacatgg cctcacattg tggcagcctt agtttggcta atagactgca tcaagataca
721 tactgccatg aaagaaagct cacctttatt tgatgatggg cagccttggg gagaagaaac
781 tgaagatgga attatgcata ataagttgtt tttggactac accataaaat gctatgagag
841 ttttatgagt ggtgccgaca gctttgatga gatgaatgca gagctgcagt caaaactgaa
901 ggatttattt aatgtggatg cttttaagct ggaatcatta gaagcaaaaa acagagcatt
961 gaatgaacag attgcaagat tggaacaaga aagagaaaaa gaaccgaatc gtctagagtc
1021 gttgagaaaa ctgaaggctt ccttacaagg agatgttcaa aagtatcagg catacatgag
1081 caatttggag tctcattcag ccattcttga ccagaaatta aatggtctca atgaggaaat
1141 tgctagagta gaactagaat gtgaaacaat aaaacaggag aacactcgac tacagaatat
1201 cattgacaac cagaagtact cagttgcaga cattgagcga ataaatcatg aaagaaatga
1261 attgcagcag actattaata aattaaccaa ggacctggaa gctgaacaac agaagttgtg
1321 gaatgaggag ttaaaatatg ccagaggcaa agaagcgatt gaaacacaat tagcagagta
1381 tcacaaattg gctagaaaat taaaacttat tcctaaaggt gctgagaatt ccaaaggtta
1441 tgactttgaa attaagttta atcccgaggc tggtgccaac tgccttgtca aatacagggc
1501 tcaagtttat gtacctctta aggaactcct gaatgaaact gaagaagaaa ttaataaagc
1561 cctaaataaa aaaatgggtt tggaggatac tttagaacaa ttgaatgcaa tgataacaga
1621 aagcaagaga agtgtgagaa ctctgaaaga agaagttcaa aagctggatg atctttacca
1681 acaaaaaatt aaggaagcag aggaagagga tgaaaaatgt gccagtgagc ttgagtcctt
1741 ggagaaacac aagcacctgc tagaaagtac tgttaaccag gggctcagtg aagctatgaa
1801 tgaattagat gctgttcagc gggaatacca actagttgtg caaaccacga ctgaagaaag
1861 acgaaaagtg ggaaataact tgcaacgtct gttagagatg gttgctacac atgttgggtc
1921 tgtagagaaa catcttgagg agcagattgc taaagttgat agagaatatg aagaatgcat
1981 gtcagaagat ctctcggaaa atattaaaga gattagagat aagtatgaga agaaagctac
2041 tctaattaag tcttctgaag aatgaagata aaatgttgat catgtatata tatccatagt
2101 gaataaaatt gtctcagtaa agtgt
//