LOCUS AK225614 2244 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for harmonin isoform a variant, clone: REC01285.
ACCESSION AK225614
VERSION AK225614.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2244)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2244
/cell_type="primary epithelial cells of human renal
proximal tubule"
/clone="REC01285"
/clone_lib="REC"
/db_xref="H-InvDB:HIT000384744"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001003274"
/organism="Homo sapiens"
/tissue_type="renal proximal tubule"
misc_feature 110..1768
/product="harmonin isoform a variant"
BASE COUNT 555 a 591 c 660 g 438 t
ORIGIN
1 agctccgagg gcggctggcc cggtcgcggt cgcggctctt tccagctcct ggcagccggg
61 cacccgaagg aacgggtcgt gcaacgacgc agctggacct ggcccagcca tggaccgaaa
121 agtggcccga gaattccggc ataaggtgga ttttctgatt gaaaatgatg cagagaagga
181 ctatctctat gatgtgctgc gaatgtacca ccagaccatg gacgtggccg tgctcgtggg
241 agacctgaag ctggtcatca atgaacccag ccgtctgcct ctgtttgatg ccattcggcc
301 gctgatccca ctgaagcacc aggtggaata tgatcagctg accccccggc gctccaggaa
361 gctgaaggag gtgcgtctgg accgtctgca ccccgaaggc ctcggcctga gtgtgcgtgg
421 tggcctggag tttggctgtg ggctcttcat ctcccacctc atcaaaggcg gtcaggcaga
481 cagcgtcggg ctccaggtag gggacgagat cgtccggatc aatggatatt ccatctcctc
541 ctgtacccat gaggaggtca acaacctcat tcgaaccaag aaaactgtgt ccatcaaagt
601 gagacacatc ggcctgatcc ccgtgaaaag ctctcctgat gagcccctca cttggcagta
661 tgtggatcag tttgtgtcgg aatctggggg cgtgcgaggc agcctgggct cccctggaaa
721 tcgggaaaac aaggagaaga aggtcttcat cagcctggta ggctcccgag gccttggctg
781 cagcatttcc agcggcccca tccagaagcc tggcatcttt atcagccatg tgaaacctgg
841 ctccctgtct gctgaggtgg gattggagat aggggaccag attgtcgaag tcaatggcgt
901 cgacttctct aacctggatc acaaggaggc tgtaaatgtg ctgaagagta gccgcagcct
961 gaccatctcc attgtagctg cagctggccg ggagctgttc atgacagacc gggagcggct
1021 ggcagaggcg cggcagcgtg agctgcagcg gcaggagctt ctcatgcaga agcggctggc
1081 gatggagtcc aacaagatcc tccaggagca gcaggagatg gagcggcaaa ggagaaaaga
1141 aattgcccag aaggcagcag aggaaaatga gagataccgg aaggagatgg aacagattgt
1201 agaggaggaa gagaagttta agaagcaatg ggaagaagac tggggctcaa aggaacagct
1261 actcttgcct aaaaccatca ctgctgaggt acacccggta ccccttcgca agccaaagta
1321 tgatcaggga gtggaacctg agctcgagcc cgcagatgac ctggatggag gcacggagga
1381 gcagggagag caggatttcc ggaaatatga ggaaggcttt gacccctact ctatgttcac
1441 cccagagcag atcatgggga aggatgtccg gctcctacgc atcaagaagg agggatcctt
1501 agacctggcc ctggaaggcg gtgtggactc ccccattggg aaggtggtcg tttctgctgt
1561 gtatgagcgg ggagctgctg agcggcatgg tggcattgtg aaaggggacg agatcatggc
1621 aatcaacggc aagattgtga cagactacac cctggctgag gctgaggctg ccctgcagaa
1681 ggcctggaat cagggcgggg actggatcga ccttgtggtt gccgtctgcc ccccaaagga
1741 gtatgacgat gagctgacct tcttctgagg tccaaaaggg gaaaccaaat tcaccgttag
1801 gaaacagtga gctccggccc cacctcgtga acacaaagcc tcggatcagc cttgagagag
1861 gccacactac acacaccaga tggcatcctt gggacctgaa tctatcaccc aggaatctca
1921 aactcccttt ggccctgaac cagggccaga taaggaacag ctcgggccac tcttctgaag
1981 gccaacgtgg aggaaaggga gcagccagcc atttgggaga agatctcaag gatccagact
2041 ctcattcctt tcctctggcc cagtgaattt ggtctctccc agctctgggg gactccttcc
2101 ttgaacccta ataagacccc actggagtct ctctctctcc atccctctcc tctgccctct
2161 gctctaattg ctgccaggat tgtcactcca aaccttactc tgagctcatt aataaaataa
2221 acagatttaa aaaaaaaaaa aaaa
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