LOCUS AK225543 3396 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for EF-hand domain (C-terminal) containing 1
variant, clone: KDN02133.
ACCESSION AK225543
VERSION AK225543.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3396)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..3396
/clone="KDN02133"
/clone_lib="KDN"
/db_xref="H-InvDB:HIT000384673"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001102853"
/organism="Homo sapiens"
/tissue_type="kidney"
misc_feature 2085..3287
/product="EF-hand domain (C-terminal) containing 1
variant"
BASE COUNT 1024 a 695 c 665 g 1012 t
ORIGIN
1 atgtttagcg ttaccatgga tcctggaggt gcccgcgaac actgcttgtc gcctgggcaa
61 ccggagagga cgaagcagga cctaggtggc ggcggtggta ccggctgcaa tggtgtccaa
121 tcccgtgcat ggcttgccct ttcttccggg cacgtccttt aaggactcta cgaaaacagc
181 cttccacaga agtcagacgc tgagctacag gaacggctat gcaattgttc gacgtccaac
241 agttgggata ggcggagacc ggctccagtt caaccagctg tcccaggctg agctggatga
301 gttggccagt aaggcaccag tcttaactta tggccaacct aaacaagccc cacctgcgga
361 ttttattcct gcgcatgtgg cctttgacaa aaaggtactg aaatttgatg cctatttcca
421 agaagatgtt cctatgtcaa ctgaggaaca gtataggatc cgtcaggtga acatttacta
481 ttatctagaa gatgacagca tgtctgtcat agagcctgtt gtagaaaatt ctggaatcct
541 tcaaggcaag ttaataaaac gccagcggct agccaagaat gaccggggtg accattacca
601 ttggaaagac ctaaatcgag gaataaacat cacaatttat ggcaaaactt tccgcgttgt
661 tgactgtgac caattcacac aggtattttt agaaagccaa ggaattgagt taaatccacc
721 agagaagatg gctcttgatc cttacactga actccgaaaa cagcctcttc gtaagtatgt
781 caccccatca gactttgatc aactcaagca atttctcacc tttgacaaac aggtaagtga
841 cataggaacc acaataggct tacttatttc caaatgtgac ctacatttat tggcaaaagg
901 tttgggtagc tgtattggta actattttga aacattacag ctataattga actgtttgga
961 cacagtactg tctttctgct ttcatcaagg gttacaggta caggaatgcc tacatttcat
1021 atggagatcc aaagaagatc gtggagttgc ggagttgttt tgtgaacctc accaaacatt
1081 taaatctcaa agcaattcct gagctacatc tgcttcccac cttacgtttc caattgacaa
1141 tttctttccc ttaaaatgag ctaatttcat agactccttt gtgaaaccat aaatcgatta
1201 ttaggaaatt tcacaaatat gcatacatgt aggttgtaat gttaaaatgt ttaatttcac
1261 agaagcccca ctacagatgc ttccttgtta aatgttatat taatattgga gtccagaatg
1321 ttctgagcat tttccaactc tgttccaacc ttcctaatcc tctcccttgt gagctgatgt
1381 gtataagcag atttaaatcc ttccctttct gtactaaagg gagaaagaaa aggaagagat
1441 caccctcagt gcttctttgc tgctcctttt ctttagacat ttaacccctt ttagttcaga
1501 aaatgtaaac tagcactagc atggtctttt aaggattttg ttcatatcag tcatatatct
1561 gttattattt tgtatttaaa gattgtgttt attcccacga tttgaagaag cctagccaaa
1621 aaaaaaaaaa aagattgtgt ttatattatt gctagaagat atgtgttgat gggaccaaaa
1681 aaagactggt taataaataa aaattttttc tacactaatt atatataaac catattcaca
1741 tgtaccttta ttaatatata tataccacta tgtaaagaac ttcattgctc ttttaattta
1801 gcttctcttt cactgactaa tattttggat caaagtgagc tcttcttttt tggcacaaac
1861 ttataatcct attatttaat tctttccagc tgctgacata tagtacataa tttcagatgt
1921 tttagtatgt ttgatgaata tttctttttt tttcaattta ccccatctga aattacttca
1981 tagtctttcc agctagtctt tccatcgttg atacataatt gccaaagtag ccaagttgaa
2041 ctccctactt ttaggattct tgagtcacta ctttggattc ttcaaaggtc cttcgattct
2101 atgcaatctg ggatgataca gacagcatgt atggtgaatg tcggacctac atcattcatt
2161 actatcttat ggatgatacg gtggaaattc gagaggtcca cgaacggaat gatgggagag
2221 atcctttccc actcctaatg aaccgccagc gtgtgcccaa agttttggtg gaaaatgcaa
2281 agaacttccc tcagtgtgtg ctagaaatct ctgaccaaga agtgttggaa tggtatactg
2341 ctaaagactt cattgttggg aagtcactca ctatccttgg gagaactttc ttcatttatg
2401 attgtgatcc atttactcga cggtattaca aagagaagtt tggaatcact gatttaccac
2461 gtattgatgt gagcaagcgg gaaccacctc cagtaaaaca ggagttgcct ccttataacg
2521 gttttggact agtggaagat tctgctcaga attgttttgc tctcattcca aaagctccaa
2581 aaaaagacgt tattaaaatg ctggtgaatg ataacaaggt gcttcgttat ttggctgtac
2641 tggaatcccc catcccagaa gacaaagacc gcagatttgt cttctcttac tttctagcta
2701 ccgacatgat cagtatcttt gagcctcctg ttcgcaattc tggtatcatt gggggcaagt
2761 accttggcag gactaaagtt gttaaaccat actctacagt ggacaaccct gtctactatg
2821 gccccagtga cttcttcatt ggtgctgtga ttgaagtgtt tggtcaccgg ttcatcatcc
2881 ttgatacaga cgagtatgtt ttgaaataca tggagagcaa cgctgcccag tattcaccag
2941 aagcactcgc gtcaattcag aaccatgtcc gaaagcgaga agcgcctgct ccagaagcag
3001 aaagcaagca aactgaaaag gatccaggcg tgcaggaatt ggaagcatta atagacacaa
3061 ttcagaagca actgaaagat cactcatgca aagacaacat tcgtgaggca tttcaaattt
3121 atgacaagga agcttcagga tatgtggaca gagacatgtt ctttaaaatc tgtgaatcgc
3181 ttaacgtccc agtggatgac tccttggtta aggaattaat caggatgtgc tctcatggag
3241 aaggcaaaat taactactat aactttgttc gtgctttctc aaactgacct gctgatgaga
3301 aaatgcaaga caatttttga tactggaact atgctttgaa atacacctta cactcttcat
3361 agaggcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
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