LOCUS AK225329 3110 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for Inter-alpha-trypsin inhibitor heavy chain H2
precursor variant, clone: HEP08701.
ACCESSION AK225329
VERSION AK225329.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3110)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..3110
/cell_line="HepG2"
/cell_type="hepatoma"
/clone="HEP08701"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000384459"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001557"
/organism="Homo sapiens"
/tissue_type="liver"
misc_feature 68..2908
/product="Inter-alpha-trypsin inhibitor heavy chain H2
precursor variant"
BASE COUNT 943 a 699 c 714 g 754 t
ORIGIN
1 agtaggaaga agtgatatcc tccccagacc atctgctttg gggagcttgg caaaactgtc
61 cagcaaaatg aaaagactca cgtgcttttt catctgcttc tttctttctg aagtatcagg
121 cttcgaaatc cccataaatg gactttctga atttgtagac tatgaagatc ttgtggaact
181 ggccccaggc aaatttcaat tggtggcaga gaaccggaga tatcagagaa gccttccagg
241 agaatcggaa gaaatgatgg aagaggttga tcaagtaact ctttatagct ataaagtcca
301 gtctactatt acttctcgga tggccaccac catgatccag agcaaagtgg tgaacaattc
361 cccgcagcct cagaatgtcg tgtttgatgt tcagatcccc aaaggagcat tcatttccaa
421 cttctccatg actgtggacg gcaagacatt taggagctct attaaggaga aaactgtggg
481 ccgagctctt tatgcacagg ccagagcaaa aggcaagacg gctggcttgg tgaggagcag
541 cgctcttgat atggaaaact tcagaacgga agtaaatgtc ctcccaggag caaaggtgca
601 gttcgaactt cactaccagg aggtgaagtg gaggaagctg ggctcctatg agcacaggat
661 ctatctgcaa cctggacggc tggccaaaca cttagaggta gatgtgtggg ttatcgaacc
721 acagggactg agatttcttc atgttcccga cacatttgaa ggccatttcg atggtgttcc
781 ggtcatttct aaaggacaac agaaggcgca cgtctccttc aagcccacgg tagcacagca
841 gagaatatgc cctagctgcc gggagactgc ggtagatggg gaactggtgg tgctgtatga
901 cgtgaaaaga gaagagaagg ctggtgaact ggaggtgttt aatggatatt ttgtccactt
961 ctttgctcct gacaacctgg acccaattcc caaaaacatc ctctttgtca tcgatgtgag
1021 tggctccatg tggggagtta aaatgaaaca aactgtggaa gcaatgaaga ccatattgga
1081 tgacctcaga gcagaagacc atttctctgt gattgatttc aaccagaaca ttcgaacttg
1141 gagaaatgat ttaatttcag ctacaaaaac acaggttgca gatgccaaga ggtatattga
1201 gaaaatccag cccagtggag gcacaaacat caacgaagca ctcctacggg caatcttcat
1261 tttgaatgaa gccaataact tgggactgtt agacctcaac tccgtctcgc tgatcattgt
1321 ggtttctgat ggagatccaa cagtgggcga actaaaactg tcaaaaattc agaaaaacgt
1381 taaggagaac atccaagaca atatctcctt gttcagtttg ggcatgggat ttgatgtgga
1441 ctatgatttt ttgaagagac tgtccaatga aaaccatgga attgcacaaa ggatttatgg
1501 aaaccaggac acgtcttccc agcttaagaa attctacaac caggtctcca ctccattgct
1561 ccggaatgtt cagttcaact atccccatac atcagtcacg gacgtcactc aaaacaattt
1621 ccataactac tttggaggct cagagattgt ggtggcagga aaatttgacc ctgctaaatt
1681 ggatcaaata gagagcgtta tcacggcgac ttcggctaac acgcagttag tcttggagac
1741 tctggcccag atggacgact tgcaggattt tctatcgaaa gacaagcatg cagatcccga
1801 tttcaccagg aaactgtggg cctatctaac catcaaccaa ctgctagctg aacgaagcct
1861 ggctcctaca gctgccgcca agagaagaat tacaagatcg atcctgcaga tgtctctaga
1921 ccaccacatt gtgactccgc tgacctcgct ggtgatcgag aacgaggctg gggatgagcg
1981 catgctggcg gatgccccac cgcaggatcc ctcctgctgc tcaggggccc tgtattacgg
2041 cagcaaagtg gttccagatt ccaccccgtc ttgggccaat ccttcaccaa cgcccgtgat
2101 ctccatgctg gcacaaggat ctcaggtgct agagtccacg ccacccccac atgtgatgag
2161 agttgaaaat gacccacatt tcatcattta tctaccaaaa agccaaaaga acatttgttt
2221 caatattgac tcagaacctg gaaaaatcct caacctggtt tctgacccag aatcaggaat
2281 tgtagtcaac ggtcagcttg ttggtgccaa gaagcccaac aatggaaaac taagcaccta
2341 ttttggaaaa ctgggatttt atttccaaag tgaagacata aaaatagaaa tcagcactga
2401 gaccatcacc ctgagccatg gttctagcac attctccttg tcctggtccg acacggctca
2461 agtcacgaat cagagggtgc agatctcagt gaagaaagaa aaagtggtaa ctatcaccct
2521 ggataaagag atgtcctttt ctgttttact tcatcgtgtt tggaagaagc atcccgtcaa
2581 tgttgacttt ctgggaatct acataccccc tacaaacaag ttctcaccta aagcccacgg
2641 actaataggc cagttcatgc aggaaccaaa gatacacatc ttcaatgaga gaccaggaaa
2701 ggaccctgag aagccagagg ccagcatgga agtgaagggg cagaagctga tcatcaccag
2761 gggcttacag aaagactaca gaacggatct agtgtttgga acggacgtta cctgctggtt
2821 tgtgcacaac agtggaaaag gattcattga cgggcattac aaggattact tcgtgcctca
2881 gctctacagc tttctcaaac ggccttaaag gtttatagtt tgggaaatta tatatattaa
2941 tatacatctt tcccctgtca cttttgcaga tattcttcgg tttgaataat taaaatgaac
3001 cagatatcag ggtggttaat taaaatgaac cagatatcag ggtggtttat aaagcctgta
3061 aacacaccta agaaaataaa cattttacaa atgaaaaaaa aaaaaaaaaa
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