LOCUS AK225322 2245 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for nucleolar protein 8 variant, clone: HEP08473.
ACCESSION AK225322
VERSION AK225322.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2245)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2245
/cell_line="HepG2"
/cell_type="hepatoma"
/clone="HEP08473"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000384452"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001101544"
/organism="Homo sapiens"
/tissue_type="liver"
misc_feature 2..1882
/product="nucleolar protein 8 variant"
BASE COUNT 828 a 403 c 508 g 506 t
ORIGIN
1 tgcggagatt gtggcttccc tgttagaagg agaggagaac acctgtggca aacagaaacc
61 aaaggaaaac aatttaaagc caaaatttca ggctttcaag ggagtaggct gtctatatga
121 aaaggagtca atgaaaaaat ccttgaaaga cagtgttgcc tctaacaata aagatcagaa
181 ttccatgaaa catgaggatc ccagtatcat atccatggaa gatgggtccc catatgttaa
241 tggctcatta ggtgaagtga ctccatggca acatgcaaag aaggcgaatg gcccaaacta
301 tattcagcct caaaaaagac agaccacttt tgaaagccag gatcgcaagg cagtgtcccc
361 tagcagttct gaaaagagaa gtaagaatcc tatttctagg ccattagaag gtaagaagtc
421 cttaagtctt agtgcaaaga ctcacaacat aggctttgac aaagacagct gccatagtac
481 cacaaagaca gaagcttcac aggaagagcg gtctgattca agcggcctca catctctcaa
541 gaaatcacca aaggtctcat ccaaggacac tcgggaaatc aaaactgatt tctcactttc
601 tattagtaat tcgtcagatg tgagtgctaa agataagcat gctgaagaca atgagaagcg
661 tttggcagcc ttggaagcga ggcaaaaagc aaaagaagtg cagaagaagc tggtgcataa
721 tgctctggca aatttggatg gtcatccaga ggataagcca acgcacatca tcttcggttc
781 tgacagtgaa tgtgaaacag aggagacatc gactcaggag cagagccatc caggagagga
841 atgggtgaaa gagtctatgg gtaaaacatc agggaagctg tttgatagca gtgatgatga
901 cgaatctgat tctgaagatg acagtaatag gttcaaaatt aaacctcagt ttgagggcag
961 agctggacag aagctcatgg atttacagtc gcactttggc accgatgaca gattccgcat
1021 ggactctcga tttctagaaa ctgacagtga agaggaacag gaagaggtaa atgaaaagaa
1081 aactgctgag gaagaagagc ttgctgagga aaaaaagaaa gccctgaatg ttgtacaaag
1141 tgttttgcaa atcaacttaa gcaattctac aaacagagga tcagtagctg ctaagaaatt
1201 taaggacatc ttacattatg atccaacgaa gcaagaccat gccacttacg aaagaaaaag
1261 agatgataag ccaagagaaa gtaaagcaaa acgaaaaaag aaaagggagg aagctgagaa
1321 actacctgag gtgtctaaag aaatgtatta taatattgct atggatctga aagaaatatt
1381 ccaaactaca aaatatacca gtgaaaagga agagggcaca ccctggaatg aggactgtgg
1441 taaagagaaa cctgaggaaa tccaggaccc tgcagctctg accagtgacg ctgagcagcc
1501 cagcgggttc acgttctctt tttttgattc agacactaaa gacataaagg aagagaccta
1561 cagagttgaa acagtgaaac ctggaaagat tgtctggcag gaagaccctc gtttacaaga
1621 cagcagttca gaagaggaag atgttactga agaaacagat cacagaaact ccagtcctgg
1681 agaagcatca ttacttgaga aagagaccac tagatttttc tttttctcta agaatgatga
1741 acgacttcaa ggttctgact tattctggag aggagtagga agtaatatga gcaggaactc
1801 ttgggaggcc agaacaacca acctgcgtat ggattgtcga aagaaacata aagacgcaaa
1861 aaggaaaatg aaaccaaaat aataaatgtc agctggtttt gatactgaat gtgaacaagg
1921 ctcacctaag gaaactgacc cagaaaacag ttttagctga caaagaagaa atttcagagt
1981 gaaggaattt taaaaatctg gctgacggaa tatcattctg gttgccatct ttttctgtgg
2041 aactcctctg catttcttcc taagtaatta cttcaaaaat taaattcaac ttcttataaa
2101 ggaagaacaa gatagtcctt gaaaatactt tttgtatata atctctttgc cctctatcct
2161 gagtaactaa tggacatctt ctcatgcaag gtttatatga agccttttta aataaatgag
2221 tcaaagcaaa aaaaaaagaa aaaaa
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