LOCUS AK225319 2688 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for Splice isoform 3 of Q96GX5 variant, clone:
HEP04584.
ACCESSION AK225319
VERSION AK225319.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2688)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2688
/cell_line="HepG2"
/cell_type="hepatoma"
/clone="HEP04584"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000384449"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001534"
/organism="Homo sapiens"
/tissue_type="liver"
misc_feature 34..2670
/product="Splice isoform 3 of Q96GX5 variant"
BASE COUNT 879 a 546 c 568 g 695 t
ORIGIN
1 agtgtacgcg gggccgctgt aggctgtcca gcgatggatc ccaccgcggg aagcaagaag
61 gagcctggag gaggcgcggc gactgaggag ggcgtgaata ggttcgcagt gccaaaaccg
121 ccctccattg aggaattcag catagtgaag cccattagcc ggggcgcctt cgggaaagtg
181 tatctggggc agaaaggcgg caaattgtat gcagtaaagg ttgttaaaaa agcagacatg
241 atcaacaaaa atatgactca tcaggtccaa gctgagagag atgcactggc actaagcaaa
301 agcccattca ttgtccattt gtattattca ctgcagtctg caaacaatgt ctacttggta
361 atggaatatc ttattggggg agatgtcaag tctctcctac atatatatgg ttattttgat
421 gaagagatgg ctgtgaaata tatttctgaa gtagcactgg ctctagacta ccttcacaga
481 catggaatca tccacaggga cttgaaaccg gacaatatgc ttatttctaa tgagggtcat
541 attaaactga cggattttgg cctttcaaaa gttactttga atagagatat taatatgatg
601 gatatcctta caacaccatc aatggcaaaa cctagacaag attattcaag aaccccagga
661 caagtgttat cgcttatcag ctcgttggga tttaacacac caattgcaga aaaaaatcaa
721 gaccctgcaa acatcctttc agcctgtctg tctgaaacat cacagctttc tcaaggactc
781 gtatgcccta tgtctgtaga tcaaaaggac actacgcctt attctagcaa attactaaaa
841 tcatgtcttg aaacagttgc ctccaaccca ggaatgcctg tgaagtgtct aacttctaat
901 ttactccagt ctaggaaaag gctggccaca tccagtgcca gtagtcaatc ccacaccttc
961 atatccagtg tggaatcaga atgccacagc agtcccaaat gggaaaaaga ttgccaggaa
1021 agtgatgaag cattgggccc aacaatgatg agttggaatg cagttgaaaa gttatgcgca
1081 aaatctgcaa atgccattga gacgaaaggt ttcaataaaa aggatctgga gttagctctt
1141 tctcccattc ataacagcag tgcccctccc accactggac gctcttgtgt aaaccttgct
1201 aaaaaatgct tctctgggga agtttcttgg gaagcagtag aactggatgt aaataatata
1261 aatatggaca ctgacacaag tcagttaggt ttccatcagt caaatcagtg ggctgtggat
1321 tctggtggga tatctgaaga gcaccttggg aaaagaagtt taaaaagaaa ttttgagttg
1381 gttgactcca gtccttgtaa aaaaattata cagaataaaa aaacttgtgt agagtataag
1441 cataacgaaa tgacaaattg ttatacaaat caaaatacag gcttaacagt tgaagtgcag
1501 gaccttaagc tatcagtgca caaaagtcaa caaaatgact gtgctaataa ggagaacatt
1561 gtcaattctt ttactgataa acaacaaaca ccagaaaaat tacctatacc aatgatagca
1621 aaaaacctta tgtgtgaact cgatgaagac tgtgaaaaga atagtaagag ggactactta
1681 agttctagtt ttctatgttc tgatgatgat agagcttcta aaaatatttc tatgaactct
1741 gattcatctt ttcctggaat ttctataatg gaaagtccat tagaaagtca gcccttagat
1801 tcagatagaa gcattaaaga atcctctttt gaagaatcaa atattgaaga tccacttatt
1861 gtaacaccag attgccaaga aaagacctca ccaaaaggtg tcgagaaccc tgctgtacaa
1921 gagagtaacc aaaaaatgtt aggtcctcct ttggaggtgc tgaaaacgtt agcctctaaa
1981 agaaatgctg ttgcttttcg aagttttaac agtcatatta atgcatccaa taactcagaa
2041 ccatccagaa tgaacatgac ttctttagat gcaatggata tttcgtgtgc ctacagtggt
2101 tcatatccca tggctataac ccctactcaa aaaagaagat cctgtatgcc acatcagacc
2161 ccaaatcaga tcaagtcggg aactccatac cgaactccga agagtgtgag aagaggggtg
2221 gcccccgttg atgatgggcg aattctagga accccagact accttgcacc tgagctgtta
2281 ctaggcaggg cccatggtcc tgcggtagac tggtgggcac ttggagtttg cttgtttgaa
2341 tttctaacag gaattccccc tttcaatgat gaaacaccac aacaagtatt ccagaatatt
2401 ctgaaaagag atatcccttg gccagaaggt gaagaaaagt tatctgataa tgctcaaagt
2461 gcagtagaaa tacttttaac cattgatgat acaaagagag ctggaatgaa agagctaaaa
2521 cgtcatcctc tcttcagtga tgtggactgg gaaaatctgc agcatcagac tatgcctttc
2581 atcccccagc cagatgatga aacagatacc tcctattttg aagccaggaa tactgctcag
2641 cacctgaccg tatctggatt tagtctgtag cacaaaaatt ttcctttt
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