LOCUS AK225314 2366 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for hypothetical protein LOC60558 variant,
clone: HEP04674.
ACCESSION AK225314
VERSION AK225314.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2366)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2366
/cell_line="HepG2"
/cell_type="hepatoma"
/clone="HEP04674"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000384444"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001513"
/organism="Homo sapiens"
/tissue_type="liver"
misc_feature 163..2172
/product="hypothetical protein LOC60558 variant"
BASE COUNT 730 a 441 c 536 g 659 t
ORIGIN
1 atctggtact tgggcagagc tccccggggt tcattgtctt cgcttcacag gatctgtttg
61 agtcctgtcc accggatcct acggggggta ccttcgaaaa aaaacgggct atgctgctgt
121 tgcgtgtggg taccctctcc tgacgcctcc gccgcccggg tcatgtggac cctcgtgggt
181 cggggctggg ggtgcgcacg cgctctcgcg ccacgagcca ctggggccgc gcttctggtg
241 gccccggggc cccggtccgc gccgaccctt ggggctgctc cagagtcctg ggctaccgac
301 aggctctaca gctccgcaga attcaaggaa aaacctgaca tgtctaggtt tcctgttgaa
361 aatattagaa atttcagtat tgttgcacac gtggatcatg gcaaaagtac tttagctgac
421 aggctcctag aacttacagg gacaattgat aaaacaaaga ataataagca ggttcttgat
481 aaattgcaag tggaacgaga aagaggaatc actgttaaag cacagacagc atctctcttt
541 tacaattgtg aaggaaagca gtacctttta aatctcattg atacaccggg ccatgttgac
601 tttagttatg aagtatccag gtcactttct gcttgccagg gtgttttact tgtggttgat
661 gcaaatgagg gaattcaagc ccaaactgta gcaaacttct ttcttgcctt cgaagcacag
721 ctatcggtaa ttccagttat aaataagata gatctgaaga atgctgatcc tgaaagggtt
781 gaaaaccaaa ttgagaaagt gtttgatatt ccaagtgatg aatgtattaa gatttctgct
841 aaacttggaa caaatgttga gagtgttctt caggcaatta ttgaaagaat cccccctcct
901 aaagtgcatc gcaaaaatcc tctgagagct ttggtatttg actccacctt tgaccagtat
961 agaggtgtga tagccaatgt agcattattt gacggagtgg tttccaaagg agataaaatt
1021 gtatctgcac atactcaaaa gacatacgaa gttaatgaag taggagtctt gaatcctaat
1081 gagcagccaa ctcataaatt atatgcagga caggtgggct atctgattgc tgggatgaaa
1141 gatgtcactg aagcgcaaat aggagataca ttatgtttac ataagcaacc agtggagccc
1201 ttgcctgggt ttaaatcagc gaaaccaatg gtatttacag gaatgtatcc tctagaccaa
1261 tctgaatata acaatctgaa gagtgctatt gaaaaactga ctttaaatga ttccagtgtg
1321 accgttcatc gggatagtag ccttgctctg ggtgctggct ggaggctagg atttcttgga
1381 cttttgcaca tggaagtttt caaccagcga ctggagcaag aatataatgc ttctgttatt
1441 ttaacaaccc ctactgttcc atataaagct gtactgtcat catcaaaatt gataaaggaa
1501 catagagaaa aagaaattac aattatcaat cctgcacaat tccccgataa atcaaaagta
1561 acagaatatt tggagccagt tgttttgggc actattatca caccagatga atacactgga
1621 aaaataatga tgctttgcga ggctcgaaga gcagttcaga agaatatgat atttattgat
1681 caaaatagag ttatgcttaa atatctcttt cctttgaatg aaattgtggt agatttttat
1741 gactctttga aatccctatc ttctggatat gctagttttg attacgaaga tgcaggctac
1801 cagactgcag aacttgtaaa aatggatatt ctactgaatg gaaatactgt agaggagcta
1861 gtaactgttg tacacaaaga caaagctcat tcaattggca aagccacatg tgaacggctg
1921 aaggattctc ttcctaggca actgtttgag atagcaattc aagctgctat tggaagtaaa
1981 atcattgcaa gagaaactgt gaaagcctat aggaaaaacg ttttggcaaa atgttatggt
2041 ggtgatatta cccgaaaaat gaagcttttg aagagacaag cagaagggaa aaaaaagctg
2101 aggaaaattg gcaacgttga agttccaaaa gatgctttta taaaagttct gaaaacacaa
2161 tcttctaaat aattggtggg aaaacaaaga attttcattg caatttgtaa tatgctgaca
2221 acagaaagaa aattataaaa tttgcttgtt actttcaggg tattcaggtt caaataacct
2281 actagtcttt cgttgaaagg gagtagttag tgggtaggca agagcttaga ttttgaagcc
2341 atgttgcctg ttctcaaata tctgtt
//