LOCUS AK225289 1888 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for dihydrolipoamide dehydrogenase precursor
variant, clone: HEP02468.
ACCESSION AK225289
VERSION AK225289.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1888)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..1888
/cell_line="HepG2"
/cell_type="hepatoma"
/clone="HEP02468"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000384419"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001370"
/organism="Homo sapiens"
/tissue_type="liver"
misc_feature 69..1598
/product="dihydrolipoamide dehydrogenase precursor
variant"
BASE COUNT 578 a 316 c 448 g 546 t
ORIGIN
1 cttggcggag cggcggaggc gcccagcgga ggtgaaagta ttggcggaaa ggaaaataca
61 gcggaaaaat gcagagctgg agtcgtgtgt actgctcctt ggccaagaga ggccatttca
121 atcgaatatc tcatggccta cagggacttt ctgcagtgcc tctgagaact tacgcagatc
181 agccgattga tgctgatgta acagttatag gttctggtcc tggaggatat gttgctgcta
241 ttaaagctgc ccagttaggc ttcaagacag tctgcattga gaaaaatgaa acacttggtg
301 gaacatgctt gaatgttggt tgtattcctt ctaaggcttt attgaacaac tctcattatt
361 accatatggc ccatggaaaa gattttgcat ctagaggaat tgaaatgtcc gaagttcgct
421 tgaatttaga caagatgatg gagcagaaga gtactgcagt aaaagcttta acaggtggaa
481 ttgcccactt attcaaacag aataaggttg ttcatgtcaa tggatatgga aagataactg
541 gcaaaaatca agtcactgct acgaaagctg atggcggcac tcaggttatt gatacaaaga
601 acattcttat agccacgggt tcagaagtta ctccttttcc tggaatcacg atagatgaag
661 atacaatagt gtcatctaca ggtgctttat ctttaaaaaa agttccagaa aagatggttg
721 ttattggtgc aggagtaata ggtgtagaat tgggttcagt ttggcaaaga cttggtgcag
781 atgtgacagc agttgaattt ttaggtcatg taggtggagt tggaattgat atggagatat
841 ctaaaaactt tcaacgcatc cttcaaaaac aggggtttaa atttaaattg aatacaaagg
901 ttactggtgc taccaagaag tcagatggaa aaattgatgt ttctattgaa gctgcttctg
961 gtggtaaagc tgaagttatc acttgtgatg tactcttggt ttgcattggc cgacgaccct
1021 ttactaagaa tttgggacta gaagagctgg gaattgaact agatcccaga ggtagaattc
1081 cagtcaatac cagatttcaa actaaaattc caaatatcta tgccattggt gatgtagctg
1141 ctggtccaat gctggctcac aaagcagagg atgaaggcat tatctgtgtt gaaggaatgg
1201 ctgccggtgc tgtgcacatt gactacaatt gtgtgccatc agtgatttac acacaccctg
1261 aagttgcttg ggttggcaaa tcagaagagc agttgaaaga agagggtatt gagtacaaag
1321 ttgggaaatt cccatttgct gctaacagca gagctaagac aaatgctgac acagatggca
1381 tggtgaagat ccttgggcag aaatcgacag acagagtact gggagcacat attcttggac
1441 caggtgctgg agaaatggta aatgaagctg ctcttgcttt ggaatatgga gcatcctgtg
1501 aagatatagc tagagtctgt catgcacatc cgaccttatc agaagctttt agagaagcaa
1561 atcttgctgc gtcattcggc aaatcaatca acttttgaat tagaagatta tatatttttt
1621 tttctgaaat ttcctgggag cttttgtaga agtcacattc ctgaacagga tattctcaca
1681 gctccaagaa tttctaggac tgaattatga aacttttgga aggtatttaa taggtttgga
1741 caaaatggaa tactcttata tctatatttt acataaattt agtattttgt ttcagtgcac
1801 taatatgtaa gacaaaaagc tacttattgt agcatcctgg aatatctccg tcaactcata
1861 ttttcatgct gttcatgaaa gattcaat
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