LOCUS AK225277 3068 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for Inter-alpha-trypsin inhibitor heavy chain H2
precursor variant, clone: HEP00535.
ACCESSION AK225277
VERSION AK225277.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3068)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..3068
/cell_line="HepG2"
/cell_type="hepatoma"
/clone="HEP00535"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000384407"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001291"
/organism="Homo sapiens"
/tissue_type="liver"
misc_feature 26..2866
/product="Inter-alpha-trypsin inhibitor heavy chain H2
precursor variant"
BASE COUNT 932 a 691 c 702 g 743 t
ORIGIN
1 gagcttggca aaactgtcca gcaaaatgaa aagactcacg tgctttttca tctgcttctt
61 tctttctgaa gtatcaggct tcgaaatccc cataaatgga ctttctgaat ttgtagacta
121 tgaagatctt gtggaactgg ccccaggcaa atttcaattg gtggcagaga accggagata
181 tcagagaagc cttccaggag aatcggaaga aatgatggaa gaggttgatc aagtaactct
241 ttatagctat aaagtccagt ctactattac ttctcggatg gccaccacca tgatccagag
301 caaagtggtg aacaattccc cgcagcctca gaatgtcgtg tttgatgttc agatccccaa
361 aggagcattc atttccaact tctccatgac tgtggacggc aagacattta ggagctctat
421 taaggagaaa actgtgggcc gagctcttta tgcacaggcc agagcaaaag gcaagacggc
481 tggcttggtg aggagcagtg ctcttgatat ggaaaacttc agaacggaag taaatgtcct
541 cccaggagca aaggtgcagt tcgaacttca ctaccaggag gtgaagtgga ggaagctggg
601 ctcctatgag cacaggatct atctgcaacc tggacggctg gccaaacact tagaggtaga
661 tgtgtgggtt atcgaaccac agggactgag atttcttcat gttcccgaca catttgaagg
721 ccatttcgat ggtgttccgg tcatttctaa aggacaacag aaggcgcacg tctccttcaa
781 gcccacggta gcacagcaga gaatatgccc tagctgccgg gagactgcgg tagatgggga
841 actggtggtg ctgtatgacg tgaaaagaga agagaaggct ggtgaactgg aggtgtttaa
901 tggatatttt gtccacttct ttgctcctga caacctggac ccaattccca aaaacatcct
961 ctttgtcatc gatgtgagtg gctccatgtg gggagttaaa atgaaacaaa ctgtggaagc
1021 aatgaagacc atattggatg acctcagagc agaagaccat ttctctgtga ttgatttcaa
1081 ccagaacatt cgaacttgga gaaatgattt aatttcagct acaaaaacac aggttgcaga
1141 tgccaagagg tatattgaga aaatccagcc cagtggaggc acaaacatca acgaagcact
1201 cctacgggca atcttcattt tgaatgaagc caataacttg ggactgttag accccaactc
1261 cgtctcgctg atcattttgg tttctgatgg agatccaaca gtgggcgaac taaaactgtc
1321 aaaaattcag aaaaacgtta aggagaacat ccaagacaat atctccttgt tcagtttggg
1381 catgggattt gatgtggact atgatttttt gaagagactg tccaatgaaa accatggaat
1441 tgcacaaagg atttatggaa accaggacac gtcttcccag cttaagaaat tctacaacca
1501 ggtctccact ccattgctcc ggaatgttca gttcaactat ccccatacat cagtcacgga
1561 cgtcactcaa aacaatttcc ataactactt tggaggctca gagattgtgg tggcaggaaa
1621 atttgaccct gctaaattgg atcaaataga gagcgttatc acggcgactt cggctaacac
1681 gcagttagtc ttggagaccc tggcccagat ggacgacttg caggattttc tatcgaaaga
1741 caagcatgca gatcccgatt tcaccaggaa actgtgggcc tatctaacca tcaaccaact
1801 gctagctgaa cgaagcctgg ctcctacagc tgccgccaag agaagaatta caagatcgat
1861 cctgcagatg tctctagacc accacattgt gactccgctg acctcgctgg tgatcgagaa
1921 cgaggctggg gatgagcgca tgctggcgga tgccccaccg caggatccct cctgctgctc
1981 aggggccctg tattacggca gcaaagtggt tccagattcc accccgtctt gggccaatcc
2041 ttcaccaacg cccgtgatct ccatgctggc acaaggatct caggtgctag agtccacgcc
2101 acccccacat gtgatgagag ttgaaaatga cccacatttc atcatttatc taccaaaaag
2161 ccaaaagaac atttgtttca atattgactc agaacctgga aaaatcctca acctggtttc
2221 tgacccagaa tcaggaattg tagtcaacgg tcagcttgtt ggtgccaaga agcccaacaa
2281 tggaaaacta agcacctatt ttggaaaact gggattttat ttccaaagtg aagacataaa
2341 aatagaaatc agcactgaga ccatcaccct gagccatggt tctagcacat tctccttgtc
2401 ctggtccgac acggctcaag tcacgaatca gagggtgcag atctcagtga agaaagaaaa
2461 agcggtaact atcaccctgg ataaagagat gtccttttct gttttacttc atcgtgtttg
2521 gaagaagcat cccgtcaatg ttgactttct gggaatctac atacccccta caaacaagtt
2581 ctcacctaaa gcccacggac taataggcca gttcatgcag gaaccaaaga tacacatctt
2641 caatgagaga ccaggaaagg accctgagaa gccagaggcc agcatggaag tgaaggggca
2701 gaagctgatc atcaccaggg gcttacagaa agactacaga acggatctag tgtttggaac
2761 ggacgttacc tgctggtttg tgcacaacag tggaaaagga ttcattgacg ggcattacaa
2821 ggattacttc gtgcctcagc tctacagctt tctcaaacgg ccttaaaggt ttatagtttg
2881 ggaaattata tatattaata tacatctttc ccctgtcact tttgcagata ttcttcggtt
2941 tgaataatta aaatgaacca gatatcaggg tggttaatta aaatgaacca gatatcaggg
3001 tggtttataa agcctgtaaa cacacctaag aaaataaaca ttttacaaat gaaaaaaaaa
3061 aaaaaaaa
//