LOCUS AK225200 3229 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for dachsous 2 isoform 1 variant, clone:
COL00577.
ACCESSION AK225200
VERSION AK225200.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3229)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..3229
/clone="COL00577"
/clone_lib="COL"
/db_xref="H-InvDB:HIT000384330"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001100990"
/organism="Homo sapiens"
/tissue_type="colon"
misc_feature 1..3048
/product="dachsous 2 isoform 1 variant"
BASE COUNT 967 a 696 c 661 g 905 t
ORIGIN
1 gaagattctt tgcctggtgt aattgtgact catgtgtcag ttcatgatgt ggatttgaat
61 tcagctttca tattcagttt tgccaaagag agtaatcctg gaaccaagtt tgctattgat
121 cagaacactg gagtggtggt gttggtgaaa acattggatt ttgaagaaat gactgaatat
181 gagctgctca tccaaatttc tgattcagtg cactacacag agggagcact tgtagtccgt
241 gtgctggatg tcaatgataa tccaccagtg ttttctcaag atttctatca ggtcacagtt
301 cctgaatcaa tacctgtggg gtattcagtg ctgactctgt cagccacaga cttagaaagc
361 aatgagaaca tttcttacag aattctatcc tcttctaagg aattctccat tgatcctaag
421 aatggcacaa tatttactat cagtcccgta ttacttctgg atacaatatc aacaactcga
481 tttcttgtgg aagccagtga tggtggaaat cctgacccga gagctcttac tttagtggag
541 ataggaatag aagatatgaa caattatgcc cctgaattca cagtcaaatc ctataatctt
601 agcctaagtg aggatgctct ggttggaagc acgcttgtta cattttcaaa catcgaccat
661 gactggaccc gtgaaaacac atatgttgaa tattccatca tcagtggtaa ttcacagaac
721 aattttcatg tggaaactaa gttctttcat tcagaatatc cttataagca agtcggttat
781 cttgtgttgc ttcacagtct ggacagagaa gcaagtgcta gccatgagct tgtcattctg
841 gcatctgaca gtggctgccc tccattgagt tccacagctg tcatatcaat acaagtactt
901 gatgtcaatg acaatccccc aaacttcagc agcctgagct atcacaccca tgtcaaggaa
961 agcacccctc tagggagtca catcactgtg gtctcagcaa atgaccgtga cacagggtca
1021 catgcagaaa tcatctacaa catcatctct ggaaatgaga agggacattt ttacttagaa
1081 gaaaacactg gagttcttta tttgattaaa cctctggatt atgaaaaaat gacaaaattc
1141 accttaactg tccaagcttc agatgcagaa aagaaacatt tttcttttgc agttgtgttt
1201 gtcagtgtcc tggatgataa cgaccatgca cctcagttta tgttctcaag cttcagctgt
1261 attgttccag aaaatctgcc tatttcctct accatatgct ctataaatgc tctggatttt
1321 gatgctggtc cgtatggaga attgacctat tctattgtat caccctgttt tctcactcat
1381 ggaatgtctt atgatcatga tctcttcctc attgaccctt tgacagggga tattcatgct
1441 aagcaaatcc ttgactatga aaatggcaat aaatactgcc tcacagtcca agccaaagac
1501 aaaggtgatg caactgcctc cttagtggtc tgggtggata ttgaagggat agatgaattt
1561 gagcccattt tcactcaaga tcagtatttt ttcaccctcc cagaaaagaa taaagacaga
1621 cagttgattg gcagagtgga agcctcagat gcagatgctg gtattgatgg agtcattctt
1681 tactcccttg gaacctcatc tcctttcttt tcagtaaata gaaccaatgg aaatatttat
1741 ttgattagag cccttcccct aataaaaagt caactcaaca aagaagacac cttggaaatg
1801 aaaataatcg ctcatagtcc caaatcagat tccaagtttg catcttgcac tgtttttgtg
1861 aatgtgtctt tctcctctga aggaacaccc ttggcagtgt tcgccagcag cttttcaatc
1921 agcctggtgg tctccttttt agtgtttctg atactcatct gcattctaat tgtaatgatt
1981 ttaagacata aacaaaaaga cacaataaac aattatgagg agaagaaaac ctcatcttta
2041 gatgcggact tgagagtgac ccgggatgcc agtgtgctca aagccttcca gaaaactgac
2101 gactgcagta acgaggtggt ccctgtggat gccactccgg aatggttgag tttaataagt
2161 atcatggaga aggatattgt caatctgtac agatactcaa actccagtgg ccactgttct
2221 gtggaaggag aaactgcaga agataaggaa atccagagga taaatgagca tccctacaga
2281 aagtgctcag actcagctct gagtgaccac gagtccaggg tgccagactc gggtatcccg
2341 agggactcag accagctctc ctgcctatct ggggaaactg atgtgatggt gactgccgaa
2401 acagcagaag ccagccaaac atttggggaa ggagatcaag gggaaggctg cagcaccacc
2461 tgtgctcaaa ataatgtgtt accccagaca gttcagaaga gagaggcaaa agagagcatc
2521 ctggctgacg ttagaaaaga gtctgtcttt atttcaggtg atcaggaagt aaggtgtgca
2581 gctctttcaa ctcagacgac ctctgatcat gatggaaaag acaactatca ctggaattat
2641 cttcttagtt gggagcccaa attccaacct cttgcctcag tatttaatga tattgcaaaa
2701 ctaaaggatg aacatttgca tatgcctggc attccaaaag agaagaaatc ttttgttttt
2761 ccaccccctt tgataacagc agtagcccag cctgggatta aagcagtccc accaagaatg
2821 ccggcagtaa acctggggca ggtgcctccg aaacacccac gctctcccat cccctaccat
2881 cttggttctc tgccagaagg catgactccc aatttttctc catctctttc cctattgacg
2941 atgcagcctc ctgccttgtc tccactgttg agagaaggag aattattagg aacacacatc
3001 agtggtacat gccatgaact taaagcagaa gatgaagttc aaatatgaaa ccactgggat
3061 gccaagtacc tgctcaccat tggtcatgaa tgaatgaaca aaatgttttc aagccggcaa
3121 ctcgagattg ggctcatttt tatctaaaag caagtgatgt aatttagtta gagtttttaa
3181 aacttcccca ttaaagtttc tccaatttca aaaaaaaaaa aaaaaaaaa
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