LOCUS AK223586 3121 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for tubulin, gamma complex associated protein 2
variant, clone: FCC130D10.
ACCESSION AK223586
VERSION AK223586.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3121)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..3121
/clone="FCC130D10"
/clone_lib="TYB_KID"
/db_xref="H-InvDB:HIT000331366"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001001199"
/organism="Homo sapiens"
/tissue_type="Kidney"
CDS <265..2970
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="tubulin, gamma complex associated protein 2
variant"
/protein_id="BAD97306.1"
/translation="MSEFRIHHDVNELLSLLRVHGGDGAEVYIDLLQKNRTPYVTTTV
SAHSAKVKIAEFSRTPEDFLKKYDELKSKNTRNLDPLVYLLSKLTEDKETLQYLQQNA
KERAELAAAAVGSSTTSINVPAAASKISMQELEELRKQLGSVATGSTLQQSLELKRKM
LRDKQNKKNSGQHLPIFPAWVYERPALIGDFLIGAGISTDTALPIGTLPLASQESAVV
EDLLYVLVGVDGRYVSAQPLAGRQSRTFLVDPNLDLSIRELVHRILPVAASYSAVTRF
IEEKSSFEYGQVNHALAAAMRTLVKEHLILVSQLEQLHRQGLLSLQKLWFYIQPAMRT
MDILASLATSVDKGECLGGSTLSLLHDRSFSYTGDSQAQELCLYLTKAASAPYFEVLE
KWIYRGIIHDPYSEFMVEEHELRKERIQEDYNDKYWDQRYTIVQQQIPSFLQKMADKI
LSTGKYLNVVRECGHDVTCPVAKEIIYTLKERAYVEQIEKAFNYASKVLLDFLMEEKE
LVAHLRSIKRYFLMDQGDFFVHFMDLAEEELRKPVEDITPPRLEALLELALRMSTANT
DPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAMAHADPTELALSGLEAFSFDYIVKW
PLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHSLHSAQWFAGAFTL
RQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLGHHTGFLDTCLKDCMLT
NPELLKVFSKLMSVCVMFTNCMQKFTQSMKLDGELGGQTLEHSTVLGLPAGAEERARK
ELARKHLAEHADTVQLVSGFEATINKFDKNFSAHLDLLARLSIYSTSDCEHGMASVIS
RLDFNGFYTERLERLSAERSQKVTPQVPVLRGPPAPAPRVAVTAQ"
BASE COUNT 724 a 916 c 904 g 577 t
ORIGIN
1 cgaaagggcg cggccccagc cgcgggatcc cgctctacag gcagcggctc cgccgccccg
61 ggccccgcag cgaggcgggc gcacagcgcg gggggcaact gcatcacccc gggcgcgggc
121 gggcgcagcc tgtcggccac cgtccgcgag ccgagccgga cgtgacgtca cgctcacttc
181 cgccgccgtc gggggctgcg cgggggctgt ggctccggga ctgcggagaa catattgtga
241 tgttcgtgcc tcagagctaa aactatgagt gaatttcgga ttcaccatga cgtcaatgaa
301 ctgcttagcc tgctgcgtgt ccacggagga gatggggctg aggtctacat tgacctgctt
361 caaaagaaca ggaccccgta cgtcactacc actgtctctg ctcacagtgc caaggttaaa
421 attgcagagt tttctcgtac tccagaagac tttctaaaga aatatgatga actgaaatct
481 aaaaatacaa ggaaccttga cccgctggtg tacctgttgt caaagctcac ggaagacaaa
541 gagactctgc agtacttaca acagaatgca aaagaaagag ctgagcttgc agccgctgct
601 gtgggcagca gtaccaccag catcaacgtc cctgccgcgg cctccaagat ctccatgcag
661 gagcttgagg aactgaggaa gcagcttggc agcgtggcca caggctccac gctgcagcag
721 tctctggaac ttaaaagaaa gatgcttcga gacaagcaga acaaaaaaaa ttcaggccag
781 cacctcccca tcttcccagc atgggtgtat gagagacctg ccctgatcgg ggatttcctg
841 attggtgctg gcatcagcac agacaccgct ttgccgatag gcacgttgcc cctggcctcg
901 caggagtcgg ccgtggtgga ggacctgctg tacgtgctgg tgggcgtgga cgggaggtac
961 gtcagtgctc agcccctggc tgggaggcag agccggacct tcctcgtgga ccccaacctg
1021 gacctgtcca tcagggagct ggtgcacagg atcctcccag tggccgccag ctactccgct
1081 gtgaccaggt tcattgaaga gaagtcttcc ttcgagtacg ggcaggtgaa ccacgccctg
1141 gcggccgcca tgcgcaccct ggtgaaggag cacctgattc tggtgtcaca gctggagcag
1201 ctgcacaggc agggcctcct ttcgctgcag aagctctggt tctacatcca gccagccatg
1261 cgcaccatgg acatcctggc ctccctcgcc acctcggtgg acaaaggcga atgtcttggg
1321 gggtccacgc tgagcctgct ccacgacagg agcttcagct acacagggga cagccaggcg
1381 caggagctat gcctgtacct aaccaaggcg gccagtgctc cctacttcga ggttctggag
1441 aagtggatct acaggggcat catccacgac ccatacagtg agtttatggt cgaggagcac
1501 gagctgcgga aggagaggat ccaggaggat tacaacgaca agtactggga ccagcggtac
1561 accatcgtcc agcagcagat cccgtccttc ctgcagaaaa tggcggacaa gatcctcagc
1621 acaggaaaat atctaaatgt ggtcagagag tgtggccatg acgtcacctg cccggtggct
1681 aaagagatca tctacacgtt aaaagagcgg gcgtatgtgg agcagatcga gaaggcgttt
1741 aactacgcca gcaaggtgct gctggacttc ctgatggagg agaaggagct ggtggctcac
1801 ctcaggtcca tcaagcgcta cttcctcatg gaccagggcg acttcttcgt gcacttcatg
1861 gacctcgcgg aggaggagct ccggaagccg gtggaggaca tcacgccccc tcgcctggaa
1921 gcgctcctgg agctggcgct gcgcatgagc acggccaaca ctgacccctt caaggacgac
1981 ctcaagatcg acctgatgcc ccatgacctc atcactcagc tcttgcgcgt cctggccatc
2041 gagaccaagc aggagaaggc gatggcgcac gccgacccca cggagctggc gctgagcggc
2101 ctggaggcct tctctttcga ctacatcgtc aagtggcccc tttcgctcat catcaacagg
2161 aaagccctca ctcgctacca gatgctcttc aggcacatgt tctattgcaa gcacgtggag
2221 cggcagctct gcagcgtctg gatcagcaac aaaaccgcca agcagcactc gctgcactcc
2281 gcccagtggt ttgctggggc tttcactctg cggcagcgaa tgctcaactt cgtccagaat
2341 attcaatact acatgatgtt cgaagtgatg gaaccgacct ggcacatcct ggagaaaaac
2401 ctgaaatccg cctccaacat tgacgacgtc cttggccacc acacaggctt cctggacacc
2461 tgcctgaagg actgcatgct caccaacccc gagctgctga aggtcttctc caagctcatg
2521 tctgtgtgcg tcatgttcac caactgcatg cagaaattta cacagagcat gaaattagat
2581 ggcgagctgg gcgggcagac gctggagcac agcaccgtcc tggggctgcc cgcaggggcc
2641 gaggagcggg cccggaagga gctcgccagg aagcacctgg ctgagcacgc agacactgtg
2701 cagctggtgt ccggcttcga ggccaccatc aacaagtttg acaagaactt ctcagcccac
2761 ctggacctcc tggcccggct gagcatctat agcaccagtg actgtgagca cggcatggcc
2821 agcgtcatct ccaggcttga cttcaatggt ttctacacgg agcgcctgga gcgcctgtct
2881 gcagagagga gccagaaggt caccccccaa gtgcctgtcc tgcgggggcc cccggctcct
2941 gcacccaggg tcgcagtcac cgcacagtga gccctggctg tgacaggaag gaagggtgtg
3001 gggtcagcag ggactggtgc aaatgggtcc agaattttca aatcgaatgc tctgtgttct
3061 gtctttgcag tttaaatata aagcaggaat gattgaaaaa aaaaaaaaaa aaaaaaaaag
3121 g
//