LOCUS AK223545 2312 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for serologically defined colon cancer antigen
33 variant, clone: FCC125H09.
ACCESSION AK223545
VERSION AK223545.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2312)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..2312
/clone="FCC125H09"
/clone_lib="TYB_KID"
/db_xref="H-InvDB:HIT000331325"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001000934"
/organism="Homo sapiens"
/tissue_type="kidney"
CDS <1..2279
/codon_start=3
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="serologically defined colon cancer antigen 33
variant"
/protein_id="BAD97265.1"
/translation="QDLSVHMIKTKHYQKVPLKEPVPAITKLVPSTKKRALQDLAPPC
SPEPAGMAAEVALSESAKDQKAANPYVTPNNRYGYQNGASYTWQFEARKAQILKCMEC
GSSHDTLQQLTAHMMVTGHFLKVTTSASKKGKQLVLDPVVEEKIQSIPLPPTTHTRLP
ASSIKKQPDSPAGSTTSEEKKEPEKEKPPVAGDTEKIKEESEDSLEKFEPSTLYPYLR
EEDLDDSPKGGLDILKSLENTVSTAISKAQNGAPSWGGYPSIHAAYQLPGTVKPLPAA
VQSVQVQPSYAGGVKSLSSAEHNALLHSPGSLTPPPHKSNVSAMEELVEKVTGKVNIK
KEERPPEKEKSSLAKAASPIAKENKDFPKTEEVSGKPQKKGPEAETGKAKKEGPLDVH
TPNGTEPLKAKVTNGCNNLGIIMDHSPEPSFINPLSALQSIMNTHLGKVSKPVSPSLD
PLAMLYKISNSMLDKPVYPATPVKQADAIDRYYYENSDQPIDLTKSKNKPLVSSVADS
VASPLRESALMDISDMVKNLTGRLTPKSSTPSTVSEKSDADGSSFEEALDELSPVHKR
KGRQSNWNPQHLLILQAQFASSLRETTEGKYIMSDLGPQERVHISKFTGLSMTTISHW
LANVKYQLRRTGGTKFLKNLDTGHPVFFCNDCASQFRTASTYISHLETHLGFSLKDLS
KLPLNQIQEQQNVSKVLTNKTLGPLGATEEDLGSTFQCKLCNRTFASKHAVKLHLSKT
HGKSPEDHLIYVTELEKQ"
BASE COUNT 594 a 710 c 633 g 375 t
ORIGIN
1 tgcaggacct cagcgtccac atgatcaaaa ccaagcatta ccagaaagtg cctctgaagg
61 agccagtgcc agccatcacc aaactggtcc cctccaccaa aaagcgggcg cttcaggacc
121 tggcgccccc ctgctcccct gagccagcag gaatggccgc agaggtggcc ctgagtgagt
181 cagccaagga tcagaaagca gcgaacccgt acgtcacgcc caataaccgc tatggctacc
241 agaatggcgc cagctacacc tggcagtttg aggcccgcaa ggcgcagatc ctcaagtgca
301 tggagtgtgg cagctcccac gacacgctgc agcagctcac cgcccacatg atggtcaccg
361 ggcacttcct gaaagtgacc acctcggctt ctaagaaggg caagcagttg gtgctggacc
421 ctgtggtgga agagaagatc cagtccatcc cactaccgcc caccacccac acgcggctgc
481 cggcctccag catcaaaaag cagcccgact ctcccgcggg gtccacgact tctgaagaaa
541 agaaagagcc agagaaggag aagccgcctg tggctggcga cacggagaag atcaaggagg
601 agagtgagga cagcttggag aaatttgagc ccagcaccct gtacccgtac ctgcgtgagg
661 aggacctgga cgacagcccc aagggagggc tggacattct caagtccctg gagaataccg
721 tctccacggc cattagcaaa gctcagaatg gtgcgccctc atggggtggc taccccagca
781 tccatgcagc ctaccagctc ccgggcaccg tgaagccact gccggcggcc gtgcagagcg
841 tgcaggtgca gccgtcctat gctggcggcg tgaagtcgct gtcttccgcc gagcacaacg
901 ccctcctgca ctccccaggg agcctcacgc ccccaccgca caagagcaac gtgtctgcca
961 tggaggagct ggtggagaag gtcacgggca aggtcaacat caagaaggag gagagacccc
1021 ctgagaagga gaagagctcc ctggccaagg ctgcgtcccc catagcaaaa gagaataaag
1081 atttcccgaa aacggaggaa gtcagcggca aaccacagaa gaagggccct gaggccgaga
1141 ctgggaaggc caaaaaggag ggaccgctgg acgttcacac cccaaatggc acagagcctc
1201 tcaaagcaaa ggtcaccaac ggctgtaaca acctggggat catcatggac cactcaccgg
1261 agccttcctt catcaacccg ctgagcgctt tgcagtccat catgaacacc cacctgggca
1321 aggtgtccaa gcccgtgagt ccctcgctgg acccgctggc gatgctgtac aagatcagca
1381 acagcatgct ggacaagccg gtgtaccccg ccacccctgt gaagcaggcc gatgccatcg
1441 accgctacta ttatgaaaat agcgaccagc ccattgactt aaccaagtcc aagaacaagc
1501 cgctggtgtc cagcgtggct gattcggtgg catcacctct gcgggagagc gcactcatgg
1561 acatctccga catggtgaaa aacctcacag gccgcctgac gcccaagtcc tccacgccct
1621 ccacagtttc agagaagtcc gatgctgatg gcagcagctt tgaggaggcg ttggacgagc
1681 tgtcaccggt ccacaagagg aagggccggc agtccaactg gaacccgcag caccttctca
1741 tcctgcaggc ccagttcgcc tcgagcttgc gggagaccac agagggcaag tacatcatgt
1801 cggacttggg cccgcaggag agggtgcaca tctcgaagtt tactgggctc tccatgacca
1861 ccatcagcca ctggctggcc aatgtgaagt accagttgag gaggacaggg ggaacgaaat
1921 tcctaaagaa cctggacaca gggcatcctg ttttcttttg caacgattgt gcctctcagt
1981 tcagaactgc ttctacatac ataagtcatt tggagacaca cttgggcttc agcctgaagg
2041 atctctccaa gctgccactc aatcagattc aagaacagca gaatgtttcg aaagtcctca
2101 ccaacaaaac tctgggccca ctgggggcca ccgaggaaga cttgggctcc acattccaat
2161 gtaagctctg caaccggact tttgcgagca agcacgcagt caaactgcac cttagtaaga
2221 cccacggcaa gtctcccgag gaccacctga tctatgtgac tgagttggag aaacagtagc
2281 gtccaggtat gcaagagacc gcggaacatt gc
//