LOCUS AK223540 1672 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for vaccinia related kinase 2 variant, clone:
FCC125C11.
ACCESSION AK223540
VERSION AK223540.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1672)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..1672
/clone="FCC125C11"
/clone_lib="IMS_TST"
/db_xref="H-InvDB:HIT000331320"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001000901"
/organism="Homo sapiens"
/tissue_type="testis"
CDS <88..1614
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="vaccinia related kinase 2 variant"
/protein_id="BAD97260.1"
/translation="MPPKRNEKYKLPIPFPEGKVLDDMEGNQWVLGKKIGSGGFGLIY
LAFPTNKPEKDARHVVKVEYQENGPLFSELKFYQRVAKKDCIKKWIERKQLDYLGIPL
FYGSGLTEFKGRSYRFMVMERLGIDLQKISGQNGTFKKSTVLQLGIRMLDVLEYIHEN
EYVHGDIKAANLLLGYKNPDQVYLADYGLSYRYCPNGNHKQYQENPRKGHNGTIEFTS
LDAHKGVALSRRSDVEILGYCMLRWLCGKLPWEQNLKDPVAVQTAKTNLLDELPQSVL
KWAPSGSSCCEIAQFLVCAHSLAYDEKPNYQALKKILNPHGIPLGPLDFSTKGQSINV
HTPNSQKVDSQKAATKQVNKAHNRLIEKKVHSERSAESCATWKVQKEEKLIGLMNNEA
AQESTRRRQKYQESQEPLNEVNSFPQKISYTQFPNSFYEPHQDFTSPDIFKKSRSPSW
YKYTSTVSTGITDLESSTGLWPTISQFTLSEETNADVYYYRIIIPVLLMLVFLALFFL
"
BASE COUNT 555 a 323 c 368 g 426 t
ORIGIN
1 gtaggcccgg gggctccgcc tcgtcgcagc ggcaggtagg aggcggtgcg cgcggcccgg
61 cgacggggga tcctgaggcc cgaagtgatg ccaccaaaaa gaaatgaaaa atacaaactt
121 cctattccat ttccagaagg caaggttctg gatgatatgg aaggcaatca gtgggtactg
181 ggcaagaaga ttggctctgg aggatttgga ttgatatatt tagctttccc cacaaataaa
241 ccagagaaag atgcaagaca tgtagtaaaa gtggaatatc aagaaaatgg cccgttattt
301 tcagaactta aattttatca gagagttgca aaaaaagact gtatcaaaaa gtggatagaa
361 cgcaaacaac ttgattattt aggaattcct ctgttttatg gatctggtct gactgaattc
421 aagggaagaa gttacagatt tatggtaatg gaaagactag gaatagattt acagaagatc
481 tcaggccaga atggtacctt taaaaagtca actgtcctgc aattaggtat ccgaatgttg
541 gatgtactgg aatatataca tgaaaatgaa tatgttcatg gtgatataaa agcagcaaat
601 ctacttttgg gttacaaaaa tccagaccag gtttatcttg cagattatgg actttcctac
661 agatattgtc ccaatgggaa ccacaaacag tatcaggaaa atcctagaaa aggccataat
721 gggacaatag agtttaccag cttggatgcc cacaagggag tagccttgtc cagacgaagt
781 gacgttgaga tcctcggcta ctgcatgctg cggtggttgt gtgggaagct tccctgggaa
841 cagaacctga aggaccctgt ggctgtgcag actgctaaaa caaatctgtt ggacgagctc
901 ccccagtcag tgcttaaatg ggctccttct ggaagcagtt gctgtgaaat agcccaattt
961 ttggtatgtg ctcatagttt agcatatgat gaaaagccaa actatcaagc cctcaagaaa
1021 attttgaacc ctcatggaat acctttagga ccactggact tttccacaaa aggacagagt
1081 ataaatgtcc atactccaaa cagtcaaaaa gttgattcac aaaaggctgc aacaaagcaa
1141 gtcaacaagg cacacaatag gttaatcgaa aaaaaagtcc acagtgagag aagcgctgag
1201 tcctgtgcaa catggaaagt gcagaaagag gagaaactga ttggattgat gaacaatgaa
1261 gcagctcagg aaagcacaag gagaagacag aaatatcaag agtctcaaga acctttgaat
1321 gaagtaaaca gtttcccaca aaaaatcagc tatacacaat tcccaaactc attttatgag
1381 cctcatcaag attttaccag tccagatata ttcaagaagt caagatctcc atcttggtat
1441 aaatacactt ccacagtcag cacggggatc acagacttag aaagttcaac tggactttgg
1501 cctacaattt cccagtttac tcttagtgaa gagacaaacg cagatgttta ttattatcgc
1561 atcatcatac ctgtcctttt gatgttagta tttcttgctt tattttttct ctgaagatga
1621 taccaaaatt ccttttgata attttttaag tttccagctc ttcaccgaaa tg
//