LOCUS AK223487 2164 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for cleavage and polyadenylation specific factor
3, 73kDa variant, clone: FCC119G02.
ACCESSION AK223487
VERSION AK223487.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2164)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..2164
/cell_line="HepG2"
/clone="FCC119G02"
/clone_lib="IMS_HEP"
/db_xref="H-InvDB:HIT000331267"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001000679"
/organism="Homo sapiens"
CDS <36..2090
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="cleavage and polyadenylation specific factor 3,
73kDa variant"
/protein_id="BAD97207.1"
/translation="MSAIPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIH
PGLEGMDALPYIDLIDPAEIDLLLISHFHLDHCGALPWFLQKTSFKGRTFMTHATKAI
YRWLLSDYVKVSNISADDMLYTETVLEESMDKIETINFHEVKEVAGIKFWCYHAGHVL
GAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTHIHEKREER
EARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSLAK
KCMAVYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMMQS
GLSRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYIS
FSAHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDNDEVHIEVHNPRN
TEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAM
STVKQTQAIPYTGPFNLLCYQLQKLTGDVEELEIQEKPALKVFKNITVIQEPGMVVLE
WLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEIMLQDIFGE
DCVSVKDDSILSVTVDGKTANLNLETRTVECEEGSEDDESLREMVELAAQRLYEALTP
VH"
BASE COUNT 681 a 427 c 481 g 575 t
ORIGIN
1 gttcctcacc cccgcttcgc cctcacactt tcgggatgtc tgcgattcct gctgaggaga
61 gcgaccagct gctgatccga ccccttggag ctgggcaaga agtaggaaga tcatgtatta
121 ttctcgagtt caaaggaaga aaaataatgc tcgactgtgg gatccaccct ggcctagaag
181 gaatggatgc tcttccttat attgatttaa ttgacccagc tgagattgat ctcctattaa
241 ttagtcattt ccatttggat cactgtggag ctctgccctg gtttctacag aagacaagtt
301 tcaaaggaag aacatttatg actcatgcca caaaagctat ttatagatgg cttctttctg
361 attatgtcaa agttagtaac atatcagcag acgacatgct gtataccgag acagttttgg
421 aagaaagcat ggacaaaatt gaaactatca actttcatga agttaaggaa gttgcgggaa
481 tcaagttttg gtgttaccat gcaggtcacg tcctaggagc cgccatgttc atgattgaga
541 tcgcaggcgt gaagcttttg tacactggtg atttctcaag acaagaagat aggcacttaa
601 tggcagctga aattcctaat attaagcctg atattcttat cattgaatct acttatggga
661 cccatatcca tgagaaacgt gaagagcgag aagcaagatt ctgtaacact gtccacgata
721 ttgtaaacag aggaggcagg ggtctcattc ctgtctttgc tcttggaagg gctcaggagc
781 tgctcttgat tctagatgag tactggcaga accacccaga actacatgac attccaatat
841 actatgcatc atctttggcc aagaagtgta tggcagtgta ccagacatat gtaaatgcca
901 tgaatgacaa aatccgcaaa cagatcaaca tcaataatcc ctttgttttc aaacacatta
961 gtaacctcaa gagcatggat cattttgatg acattggtcc cagtgttgta atggcctccc
1021 caggcatgat gcaaagtggc ttatccagag aattatttga aagctggtgt actgataaga
1081 ggaatggtgt cattatagcg ggatactgtg tagaagggac acttgccaag cacatcatgt
1141 ctgaacctga agaaatcact actatgtctg gacagaagtt accactgaaa atgtctgttg
1201 attacatttc tttctcagct cacacggatt accagcaaac cagtgaattt attcgtgctt
1261 tgaaaccgcc tcatgtgatt ttagtccatg gagaacagaa tgaaatggcc agattgaaag
1321 cagcactgat tcgagaatat gaagataacg atgaagttca catagaggtt cataatcctc
1381 ggaatacaga agcagtgacc ttaaacttca gaggagaaaa actagccaag gttatgggat
1441 ttttagcaga caaaaaacca gaacaaggcc agcgggtctc aggaatactt gttaaaagaa
1501 actttaatta tcacatactt tctccttgcg acctgtccaa ttatactgac ctggccatga
1561 gcacggtgaa gcagacccaa gccattccat atactggtcc ctttaatttg ctctgttacc
1621 agctgcagaa attgacaggt gatgtggaag aattagaaat tcaagaaaaa cctgctctga
1681 aagtgttcaa aaatattact gtaatacaag aaccaggcat ggtggtatta gaatggctgg
1741 caaacccttc taatgatatg tatgcagata cagtaacaac tgtgatattg gaagttcagt
1801 caaatcccaa aataagaaaa ggtgcagtac agaaggtttc taaaaaatta gaaatgcacg
1861 tttacagcaa gaggttggag atcatgctcc aggacatatt tggagaagac tgtgtaagtg
1921 taaaggatga ctctattctt agcgtcacag tggacgggaa aactgccaac cttaacttgg
1981 agacacggac tgtagaatgt gaagagggaa gtgaagacga tgaatccctc cgagaaatgg
2041 tggagctggc tgcacagaga ctgtacgagg ccctgacgcc agttcactga gactgtgcct
2101 gtatatgaac tttgaaaaaa tacttgactc tacttttgtt acctaaaata aaatgcattc
2161 gttt
//