LOCUS AK223460 2269 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for enoyl-Coenzyme A, hydratase/3-hydroxyacyl
Coenzyme A dehydrogenase variant, clone: FCC116B08.
ACCESSION AK223460
VERSION AK223460.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2269)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..2269
/clone="FCC116B08"
/clone_lib="IMS_KDN"
/db_xref="H-InvDB:HIT000331240"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001000541"
/organism="Homo sapiens"
/tissue_type="kidney"
CDS <41..2212
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="enoyl-Coenzyme A, hydratase/3-hydroxyacyl
Coenzyme A dehydrogenase variant"
/protein_id="BAD97180.1"
/translation="MAEYTRLHNALALIRLRNPPVNAISTTLLRDIKEGLQKAVIDHT
IKAIVICGAEGKFSAGADIRGFSAPRTFGLTLGHVVDEIQRNEKPVVAAIQGMAFGGG
LELALGCHYRIAHAEAQVGLPEVTLGLLPGARGTQLLPRLTGVPAALDLITSGRRILA
DEALKLGILDKVVNSDPVEEAIRFAQRVSDQPLESRRLCNKPIQSLPNMDSIFSEALL
KMRRQHPGCLAQEACVRAVQAAVQYPYEVGIKKEEELFLYLLQSGQARALQYAFFAER
KANKWSTPSGASWKTASARPVSSVGVVGLGTMGRGIVISFARARIPVIAVDSDKNQLA
TANKMITSVLEKEASKMQQSGHPWSGPKPRLTSSVKELGGVDLVIEAVFEEMSLKKQV
FAELSAVCKPEAFLCTNTSALDVDEIASSTDRPHLVIGTHFFSPAHVMKLLEVIPSQY
SSPTTIATVMNLSKKIKKIGVVVGNCFGFVGNRMLNPYYNQAYFLLEEGSKPEEVDQV
LEEFGFKMGPFRVSDLAGLDVGWKSRKGQGLTGPTLLPGTPARKRGNRRYCPIPDVLC
ELGRFGQKTGKGWYQYDKPLGRIHKPDPWLSKFLSRYRKTHHIEPRTISQDEILERCL
YSLINEAFRILGEGIAASPEHIDVVYLHGYGWPRHKGGPMFYASTVGLPTVLEKLQKY
YRQNPDIPQLEPSDYLKKLASQGNPPLKEWQSLAGSPSSKL"
BASE COUNT 614 a 509 c 571 g 575 t
ORIGIN
1 tgttggccta ctgggaggtg ccctcggtga tagaggaaac atggccgagt atacgcggct
61 gcacaacgcc ttggcgctaa tccgcctccg aaacccgccg gtcaacgcga tcagtacgac
121 tttactccgt gacataaaag aaggactaca gaaagctgta atagaccata caataaaagc
181 cattgtgatt tgtggagcag agggcaaatt ttctgcaggt gctgatattc gtggcttcag
241 tgctcctagg acatttggcc ttacactggg acatgtagta gatgaaatac agagaaatga
301 gaagcccgtg gtggcagcaa tccaaggcat ggctttcgga gggggactag agctggccct
361 gggctgtcac tataggattg cccacgcaga ggctcaagtt ggcttaccag aagttacact
421 gggacttctc cctggtgcaa gaggaaccca gcttctcccc agactcactg gagttcctgc
481 tgcacttgac ttaattacct caggaagacg tattttagca gatgaagcac tcaagctggg
541 cattctagat aaagttgtaa actcagaccc ggttgaagaa gcaatcagat ttgctcagag
601 agtttcagat caacctctag aatcccgtag actctgcaac aagccaattc agagcttgcc
661 caacatggac agcattttta gtgaggccct cttgaagatg cggaggcagc accctgggtg
721 tcttgcacag gaggcttgtg tccgtgcagt ccaggctgct gtgcagtatc cctatgaagt
781 gggcatcaag aaggaggagg agctgtttct atatcttttg caatcagggc aggctagagc
841 cctgcaatat gctttcttcg ctgaaaggaa agcaaataag tggtcaactc cctccggagc
901 atcgtggaaa acagcatcag cgcggcctgt ctcctcagtt ggtgttgttg gcttgggaac
961 aatgggccga ggcattgtca tttcttttgc aagggccagg attcctgtga ttgctgtaga
1021 ctcggacaaa aaccagctag caactgcaaa caagatgata acctctgtct tggaaaaaga
1081 agcctccaaa atgcaacaga gcggccaccc ttggtcagga ccaaaaccca ggttaacttc
1141 atctgtgaag gagcttggtg gtgtagattt agtcattgaa gcagtatttg aggaaatgag
1201 cctgaagaag caggtctttg ctgaactctc agctgtgtgc aaaccagaag catttttgtg
1261 cactaatact tcagccctgg atgttgatga gattgcttct tccactgatc gtcctcactt
1321 ggtcattggc acccacttct tttcgccagc tcatgtcatg aagttgttag aggttattcc
1381 cagccaatac tcttccccca ctaccattgc cactgttatg aacttatcaa aaaagattaa
1441 aaagattgga gtcgttgtag gcaactgttt tggatttgtg gggaatcgaa tgttgaatcc
1501 ttactacaat caggcatatt tcttgttaga agaaggcagc aaaccagagg aggtagatca
1561 ggtgctggaa gagtttggtt ttaaaatggg accttttaga gtgtctgatc ttgctgggtt
1621 ggatgtgggc tggaaatcta gaaaggggca aggtcttact ggacctacat tgcttccagg
1681 aactcctgcc cgaaaaaggg gtaataggag gtactgccca attcctgatg tgctctgtga
1741 attaggacga tttggccaga agacaggtaa gggttggtat caatatgaca agccattggg
1801 taggattcac aaacctgatc cctggctttc caaattccta tcacggtata gaaaaaccca
1861 tcacattgaa ccacgtacca ttagccagga tgagatcctt gaacgctgct tatattcact
1921 tatcaatgaa gcattccgta tcttgggaga agggatagct gctagcccag agcacattga
1981 tgttgtctat ttacatggat atggatggcc aaggcacaag ggcgggccca tgttctatgc
2041 ttccacagtt gggttgccca cagttctaga gaaattgcag aaatattaca ggcagaaccc
2101 tgatattccc caactggagc caagtgacta tctaaaaaaa ctggcttctc agggaaaccc
2161 tcccctgaaa gaatggcaaa gcttggcagg ctcccctagc agtaaattgt gattcagtct
2221 tccagattat gcctcacatg ctagcatcag gtaatgctga ctgaatttc
//