LOCUS AK223450 1521 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for metallophosphoesterase 1 variant, clone:
FCC114A10.
ACCESSION AK223450
VERSION AK223450.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1521)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..1521
/clone="FCC114A10"
/clone_lib="IMS_DMC"
/db_xref="H-InvDB:HIT000331230"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001000444"
/organism="Homo sapiens"
/tissue_type="dermoid cancer"
CDS <170..1360
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="metallophosphoesterase 1 variant"
/protein_id="BAD97170.1"
/translation="MAMIELGFGRQNFHPLKRKSSLLLKLIAVVFAVLLFCEFLIYYL
AIFQCNWPEVKTTASDGEQTTREPVLKAMFLADTHLLGEFLGHWLDKLRREWQMERAF
QTALWLLQPEVVFILGDIFDEGKWSTPEAWADDVERFQKMFRHPSHVQLKVVAGNHDI
GFHYEMNTYKVERFEKVFSSERLFSWKGINFVMVNSVALNGDGCGICSETEAELIEVS
HRLNCSREARGSSRCGPGPLLPTSAPVLLQHYPLYRRSDANCSGEDAAPPEERDIPFK
ENYDVLSREASQKLLWWLQPRLVLSGHTHSACEVHHGGRVPELSVPSFSWRNRNNPSF
IMGSITPTDYTLSKCYLPREDVVLIIYCGVVGFLVVLTLTHFGLLASPFLSGLNLLGK
RKTR"
BASE COUNT 378 a 354 c 395 g 394 t
ORIGIN
1 gccaacttag agcctcgtac caatctctct gtctttaaaa gatgaggtga cttggtgatt
61 ttcctggaaa attataggtg cccagctaag acctgaatgc catcaccctc cccagggctc
121 tgcagttttc tcgtggtgaa cccttgatgg atttgttgtt gcttgagaaa tggcgatgat
181 cgaattgggg tttggaagac agaattttca tccattaaag aggaagagtt cattgctgtt
241 gaaactcata gctgttgtct ttgctgtgct tctattttgt gaatttttaa tctattactt
301 agcgatcttt cagtgtaatt ggcctgaagt gaaaaccaca gcctctgatg gtgaacagac
361 cacacgtgag cctgtgctca aagccatgtt tttggctgac acccatttgc ttggggaatt
421 cctaggccac tggctggaca aattacgaag ggaatggcag atggagagag cgttccagac
481 agctctgtgg ttgctgcagc cggaagtcgt cttcatcctg ggggatatct ttgatgaagg
541 gaagtggagc acccctgagg cctgggcgga tgatgtggag cggtttcaga aaatgttcag
601 acacccaagt catgtacagc tgaaggtagt tgctggaaac catgacattg gcttccatta
661 tgagatgaac acatacaaag tagaacgctt tgagaaagtg ttcagctctg aaagactgtt
721 ttcttggaaa ggcattaact ttgtgatggt caacagcgtg gcgctgaacg gggatggctg
781 tggcatctgc tctgaaacag aagcagagct cattgaagtt tctcacagac tgaactgctc
841 ccgagaggca cgtggctcca gccggtgtgg acctgggcct ctgctgccca cgtctgcccc
901 tgtcctcctg cagcattatc ctctgtatcg gagaagtgat gctaactgtt ctggggaaga
961 cgctgctcct ccagaggaaa gggacatccc atttaaggag aactatgacg tgctttcacg
1021 ggaggcatca caaaagctgc tgtggtggct ccagccgcgc ctggttctca gtggccacac
1081 gcacagcgcc tgcgaggtgc accacggggg ccgagtcccc gagctcagcg tcccatcttt
1141 cagttggagg aacagaaaca accccagttt catcatgggt agcatcacgc ccacagacta
1201 caccctctcc aagtgctacc tcccacgtga ggatgtggtt ttgatcatct actgtggagt
1261 ggtgggcttc cttgtggtcc tcacactcac tcactttggg cttctagcct caccttttct
1321 ttctggtttg aacttgctcg gaaagcgtaa gacaagatga agagcaggcg ccattataaa
1381 tatcaaagcc caagaaatgg aactttgggc agagatcatg ttagaatcaa gtggatgatg
1441 agaccaatta caggccgtct ctctgcacag cacagaaatt ctcaatcact gaaatgagta
1501 actgcaaaat aaatagttga t
//