LOCUS AK223409 2576 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for BRCA1 associated RING domain 1 variant,
clone: FCC109A11.
ACCESSION AK223409
VERSION AK223409.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2576)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..2576
/cell_type="coronary artery endothelial cells"
/clone="FCC109A11"
/clone_lib="IMS_CAS"
/db_xref="H-InvDB:HIT000331189"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001000251"
/organism="Homo sapiens"
CDS <137..2470
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="BRCA1 associated RING domain 1 variant"
/protein_id="BAD97129.1"
/translation="MPDNRQPRNRQPRIRSGNEPRSASAMEPDGRGAWAHSRAALDRL
EKLLRCSRCTNILREPVCLGGCEHIFCSNCVSDYIGTGCPVCYTPAWIQDLKINRQLD
SMIQLCSKLRNLLHDNELSDLKEDKPRKGLFNDAGNKKNSIKMWFSPRSKKVRYVVSK
ASVQTQPAIKKDASAQQDSYEFVSPSPPADVSERAKKASARSGKKQKKKTLAEINQKW
NLEAEKEDGEFDSKEESKQKLVSFCSQPSVISSPQINGEIDLLASGSLTESECFGSLT
EVSLPLAEQIESPDTKSRNEVVTPEKVCKNYLTSKKSLPLENNGKRGHHNRLSSPISK
RCRTSILSTSGDFVKQTVPSENIPLPECSSPPSCKRKVGGTSGSKNSNMSDEFISLSP
GTPPSTLSSSSYRRVMSSPSAMKLLPNMAVKRNHRGETLLHIASIKGDIPSVEYLLQN
GSDPNVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAAKNGHVD
IVKLLLSYGASRNAVNIFGLRPVDYTDDESMKSLLLLPEKNESSSASHCSVMNTGQRR
DGPLVLIGSGLSSEQQKMLSGLAVILKAKKYTEFDSTVTHVVVPGDAVQSTLKCMLGI
LNGCWILKFEWVKACLRRKVCEQEEKYEIPEGPRRSRLNREQLLPKLFDGCYFYLWGT
FKHHPKDNLIKLVTAGGGQILSRKPKPDSDVTQTINTVAYHARPDSDQRFCTQYIIYE
DLCNYHPERVRQGKVWKAPSSWFIDCVTSFELLPLDS"
BASE COUNT 764 a 544 c 608 g 660 t
ORIGIN
1 acctctggcg gcccgccgtc ccagacgcgg gaagagcttg gccggtttcg agtcgctgac
61 ctgcagcttc cctgtggttt cccgaggcct ccttgcttcc cgctctccga ggagcctttc
121 atccgaaggc gggacgatgc cggataatcg gcagccgagg aaccggcagc cgaggatccg
181 ctccgggaac gagcctcgtt ccgcgtccgc catggaaccg gatggtcgcg gtgcctgggc
241 ccacagtcgc gccgcgctcg accgcctgga gaagctgctg cgctgctcgc gttgtactaa
301 cattctgaga gagcctgtgt gtttaggagg atgtgagcac atcttctgta gtaattgtgt
361 aagtgactac attggaactg gatgtccagt gtgttacacc ccggcctgga tacaagactt
421 gaagataaat agacaactgg acagcatgat tcaactttgt agtaagcttc gaaatttgct
481 acatgacaat gagctgtcag atttgaaaga agataaacct aggaaaggtt tgtttaatga
541 tgcagggaac aagaagaatt caattaaaat gtggtttagc cctcgaagta agaaagtcag
601 atatgttgtg agtaaagctt cagtgcaaac ccagcctgca ataaaaaaag atgcaagtgc
661 tcagcaagac tcatatgaat ttgtttcccc aagtcctcct gcagatgttt ctgagagggc
721 taaaaaggct tctgcaagat ctggaaaaaa gcaaaaaaag aaaactttag ctgaaatcaa
781 ccaaaaatgg aatttagagg cagaaaaaga agatggtgaa tttgactcca aagaggaatc
841 taagcaaaag ctggtatcct tctgtagcca accatctgtt atctccagtc ctcagataaa
901 tggtgaaata gacttactag caagtggctc cttgacagaa tctgaatgtt ttggaagttt
961 aactgaagtc tctttaccat tggctgagca aatagagtct ccagacacta agagcaggaa
1021 tgaagtagtg actcctgaga aggtctgcaa aaattatctt acatctaaga aatctttgcc
1081 attagaaaat aatggaaaac gtggccatca caatagactt tccagtccca tttctaagag
1141 atgtagaacc agcattctga gcaccagtgg agattttgtt aagcaaacgg tgccctcaga
1201 aaatatacca ttgcctgaat gttcttcacc accttcatgc aaacgtaaag ttggtggtac
1261 atcagggagc aaaaacagta acatgtccga tgaattcatt agtctttcac caggtacacc
1321 accttctaca ttaagtagtt caagttacag gcgagtgatg tctagtccct cagcaatgaa
1381 gctgttgccc aatatggctg tgaaaagaaa tcatagagga gagactttgc tccatattgc
1441 ttctattaag ggcgacatac cttctgttga atacctttta caaaatggaa gtgatccaaa
1501 tgttaaagac catgctggat ggacaccatt gcatgaagct tgcaatcatg ggcacctgaa
1561 ggtagtggaa ttattgctcc agcataaggc attggtgaac accaccgggt atcaaaatga
1621 ctcaccactt cacgatgcag ccaagaatgg gcacgtggat atagtcaagc tgttactttc
1681 ctatggagcc tccagaaatg ctgttaatat atttggtctg cggcctgtcg attatacaga
1741 tgatgaaagt atgaaatcgc tattgctgct accagagaag aatgaatcat cctcagctag
1801 ccactgctca gtaatgaaca ctgggcagcg tagggatgga cctcttgtac ttataggcag
1861 tgggctgtct tcagaacaac agaaaatgct cagtgggctt gcagtaattc ttaaggctaa
1921 aaaatatact gagtttgaca gtacagtaac tcatgttgtt gttcctggtg atgcagttca
1981 aagtaccttg aagtgtatgc ttgggattct caatggatgc tggattctaa aatttgaatg
2041 ggtaaaagca tgtctacgaa gaaaagtatg tgaacaggaa gaaaagtatg aaattcctga
2101 aggtccacgc agaagcaggc tcaacagaga acagctgttg ccaaagctat ttgatggatg
2161 ctacttctat ttgtggggaa ccttcaaaca ccatccaaag gacaacctta ttaagctcgt
2221 cactgcaggt gggggccaga tcctcagtag aaagcccaag ccagacagtg acgtgactca
2281 gaccatcaat acagtcgcat accatgcgag acccgattct gatcagcgct tctgcacaca
2341 gtatatcatc tatgaagatt tgtgtaatta tcacccagag agggttcggc agggcaaagt
2401 ctggaaggct ccttcgagct ggtttataga ctgtgtgacg tcctttgagt tgcttcctct
2461 tgacagctga atattatacc agatgaacat ttcaaattga atttgcacgg tttgtgagag
2521 cccagtcatt gtactgtttt taatgttcac atttttacaa ataggtagag tcattc
//