LOCUS AK223403 1910 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for bleomycin hydrolase variant, clone:
FCC108C02.
ACCESSION AK223403
VERSION AK223403.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1910)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..1910
/cell_line="HepG2"
/clone="FCC108C02"
/clone_lib="IMS_HEP"
/db_xref="H-InvDB:HIT000331183"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001000231"
/organism="Homo sapiens"
CDS <128..1495
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="bleomycin hydrolase variant"
/protein_id="BAD97123.1"
/translation="MSSSGLNSEKVAALIQKLNSDPQFVLAQNVGTTHDLLDICLKRA
TVQRAQHVFQHAVPQEGKPITNQKSSGRCWIFSCLNVMRLPFMKKLNIEEFEFSQSYL
FFWDKVERCYFFLSAFVDTAQRKEPEDGRLVQFLLMNPVNDGGQWDMLVNIVEKYGVI
PKKCFPESYTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISATQDVMMEEIFR
VVCICLGNPPETFTWEYRDKDKNYQKIGPITPLEFYREHVKPLFNMEDKICLVNDPRP
QHKYNKLYTVEYLSNMVGGRKTLYNNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNS
KLGLSDMNLYDHELVFGVSLKNMNKAERLTFGESLMTHAMTFTAVSEKDDQDGAFTKW
RVENSWGEDHGHKGYLCMTDEWFSEYVYEVVVDRKHVPEEVLAVLEQEPIILPAWDPM
GALAE"
BASE COUNT 527 a 408 c 504 g 471 t
ORIGIN
1 ttttttttcc ggcgctccgg gtgcgagaga caggtcgggc cccctaggca gcgagccgca
61 gcgcaatccc ggcgctcgcc caaggaccct ggaagctacc gttaccccgc cgggcagcgt
121 gggcgccatg agcagctcgg gactgaattc ggagaaggta gctgctctga tacagaaact
181 gaattccgac ccccagttcg tacttgccca gaatgtcggg accacccacg acctgctgga
241 catctgtctg aagcgggcca cggtgcagcg cgcgcagcat gtgttccagc acgccgtgcc
301 ccaggagggc aagccaatca ccaaccagaa gagctcaggg cgatgctgga tcttttcttg
361 tctgaatgtt atgaggcttc cattcatgaa aaagttaaat attgaagaat ttgagtttag
421 ccaatcttac ctgttttttt gggacaaggt tgaacgctgt tatttcttct tgagtgcttt
481 tgtggacaca gcccagagaa aggagcctga ggatgggagg ctggtgcagt ttttgcttat
541 gaaccctgta aatgatggtg gccaatggga tatgcttgtt aatattgttg aaaaatatgg
601 tgttatccct aagaaatgct tccctgaatc ttatacaaca gaggcaacca gaaggatgaa
661 tgatattctg aatcacaaga tgagagaatt ctgtatacga ctgcggaacc tggtacacag
721 tggagcaacc aaaggagaaa tctcggccac acaggacgtc atgatggagg agatattccg
781 agtggtgtgc atctgtttgg gtaatccacc agagacattc acctgggaat atcgagacaa
841 agataaaaat tatcagaaaa ttggccccat aacacccttg gagttttaca gggaacatgt
901 caagccactc ttcaatatgg aagataagat ttgtttagtg aatgacccta ggccccagca
961 caagtacaac aaactttaca cagtggaata cttaagcaat atggttggag ggagaaaaac
1021 tctatacaac aaccagccca ttgacttcct gaaaaagatg gttgctgcct ccatcaaaga
1081 tggagaggct gtgtggtttg gctgtgatgt tggaaaacac ttcaatagca agctgggcct
1141 cagtgacatg aatctctatg accatgagtt agtgtttggt gtctccttga agaacatgaa
1201 taaagcggag aggctgactt ttggtgagtc acttatgacc cacgccatga ccttcactgc
1261 tgtctcagag aaggatgatc aggatggtgc tttcacaaaa tggagagtgg agaattcatg
1321 gggtgaagac catggccaca aaggttacct gtgcatgaca gatgagtggt tctctgagta
1381 tgtctacgaa gtggtggtgg acaggaagca tgtccctgaa gaggtgctag ctgtgttaga
1441 gcaggaaccc attatcctgc cagcatggga ccccatggga gctttggctg agtgatactg
1501 ccctccagct ctttcctcct tccatggaac ctgacgtagc tgcaaaggac agatccaggg
1561 actgaagcca aagttatgca agggactgtg tgttgccaca ggacacagtc agatttccag
1621 tctccaccag gaacctcttc agaaagtgtg ctttatgctg aaacagaata ctgttaaagg
1681 aaaaaaaaga ggggggaaga tcaggtcata ctatctactc tcctcatctc taacagctca
1741 ggatctctta gcattttaat tagatgtaat tgtttgtctt taactgtcaa aaagtttggt
1801 tctgtgtctg tgttttaata agacgagagg acgagcgatt gaggtgtatg gagagaaaac
1861 agacctaatg ctccttgttc ctagagtaga gtggagggag ggtggcctaa
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