LOCUS AK223399 2308 bp mRNA linear HUM 26-APR-2005
DEFINITION Homo sapiens mRNA for guanine monophosphate synthetase variant,
clone: FCC107G06.
ACCESSION AK223399
VERSION AK223399.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2308)
AUTHORS Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y., Tanaka,A. and
Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
FEATURES Location/Qualifiers
source 1..2308
/cell_line="HepG2"
/clone="FCC107G06"
/clone_lib="IMS_HEP"
/db_xref="H-InvDB:HIT000331179"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hst001000220"
/organism="Homo sapiens"
CDS <219..2300
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="guanine monophosphate synthetase variant"
/protein_id="BAD97119.1"
/translation="MALCNGDSKLENAGGDLKDGHHHYEGAVVILDAGAQYGKVIDRR
VRELFVQSEIFPLETPAFAIEEQGFRAIIISGGPNSVYAEDAPWFDPAIFTIGKPVLG
ICYGMQMMNKVFGGTVHKKSVREDGVFNISVDNTCSLFRGLQKEEVVLLTHGDSVDKV
ADGFKVVARSGNIVAGIANESKKLYGAQFHPEVGLTENGKVILKNFLYDIAGCSGTFT
VQNRELECIREIKERVGTSKVLVLLSGGVDSTVCTALLNRALNQEQVIAVHIDNGFMR
KRESQSVEEALKKLGIQVKVINAAHSFYNGTTTLPISDEDRTPRKRISKTLNMTTSPE
EKRKIIGDTFVKIANEVIGEMNLKPEEVFLAQGTLRPDLIESASLVASGKAELIKTHH
NDTELIRKLREEGKVIEPLKDFHKDEVRILGRELGLPEELVSRHPFPGPGLAIRVICA
EEPYICKDFPETNNILKIVADFSASVKKPHTLLQRVKACTTEEDQEKLMQITSLHSLN
AFLLPIKTVGVQGDCRSYSYVCGISSKDEPDWESLIFLARLIPRMCHNVNRVVYIFGP
PVKEPPTDVTPTFLTAGVLSTLRQADFEAHNILREPGYAGKISQMPVILTPLHFDRDP
LQKQPSCQRSVVIRTFITSDFMTGIPATPGNEIPVEVVLKMVTEIKKIPGISRIMYDL
TSKPPGTTEWE"
BASE COUNT 659 a 493 c 549 g 607 t
ORIGIN
1 gtgcgcgctg ctggtcttct ctcccgcggc gctggggccc gcgctccgct gctgttgctc
61 cattcggcgc ttttctggcg gctggctcct ctccgctgcc ggctgctcct cgaccaggcc
121 tccttctcaa cctcagcccg cggcgccgac ccttccggca ccctcccgcc ccgtctcgta
181 ctgtcgccgt caccgtcgcg gctccggccc tggccccgat ggctctgtgc aacggagact
241 ccaagctgga gaatgctgga ggagacctta aggatggcca ccaccactat gaaggagctg
301 ttgtcattct ggatgctggt gctcagtacg ggaaagtcat agaccgaaga gtgagggaac
361 tgttcgtgca gtctgaaatt ttccccttgg aaacaccagc atttgctata gaggaacaag
421 gattccgtgc tattatcatc tctggaggac ctaattctgt gtatgctgaa gatgctccct
481 ggtttgatcc agcaatattc actattggca agcctgttct tggaatttgc tatggtatgc
541 agatgatgaa taaggtattt ggaggtactg tgcacaaaaa aagtgtcaga gaagatggag
601 ttttcaacat tagtgtggat aatacatgtt cattattcag gggccttcag aaggaagaag
661 ttgttttgct tacacatgga gatagtgtag acaaagtagc tgatggattc aaggttgtgg
721 cacgttctgg aaacatagta gcaggcatag caaatgaatc taaaaagtta tatggagcac
781 agttccaccc tgaagttggc cttacagaaa atggaaaagt aatactgaag aatttccttt
841 atgatatagc tggatgcagt ggaaccttca ccgtgcagaa cagagaactt gagtgtattc
901 gagagatcaa agagagagta ggcacgtcaa aagttttggt tttactcagt ggtggagtag
961 actcaacagt ttgtacagct ttgctaaatc gtgctttgaa ccaagaacaa gtcattgctg
1021 tgcacattga taatggcttt atgagaaaac gagaaagcca gtctgttgaa gaggccctca
1081 aaaagcttgg aattcaggtc aaagtgataa atgctgctca ttctttctac aatggaacaa
1141 caaccctacc aatatcagat gaagatagaa ccccacggaa aagaattagc aaaacgttaa
1201 atatgaccac aagtcctgaa gagaaaagaa aaatcattgg ggatactttt gttaagattg
1261 ccaatgaagt aattggagaa atgaacttga aaccagagga ggttttcctt gcccaaggta
1321 ctttacggcc tgatctaatt gaaagtgcat cccttgttgc aagtggcaaa gctgaactca
1381 tcaaaaccca tcacaatgac acagagctca tcagaaagtt gagagaggag ggaaaagtaa
1441 tagaacctct gaaagatttt cataaagatg aagtgagaat tttgggcaga gaacttggac
1501 ttccagaaga gttagtttcc aggcatccat ttccgggtcc tggcctggca atcagagtaa
1561 tatgtgctga agaaccttat atttgtaagg actttcctga aaccaacaat attttgaaaa
1621 tagtagctga tttttctgca agtgttaaaa agccacatac cctattacag agagtcaaag
1681 cctgcacaac agaagaggat caggagaagc tgatgcaaat taccagtctg cattcactga
1741 atgccttctt gctgccaatt aaaactgtag gtgtgcaggg tgactgtcgt tcctacagtt
1801 acgtgtgtgg aatctccagt aaagatgaac ctgactggga atcacttatt tttctggcta
1861 ggcttatacc tcgcatgtgt cacaacgtta acagagttgt ttatatattt ggcccaccag
1921 ttaaagaacc tcctacagat gttactccca ctttcttgac agcaggggtg ctcagtactt
1981 tacgccaagc tgattttgag gcccataaca ttctcaggga gcctgggtat gctgggaaaa
2041 tcagccagat gccggtgatt ttgacaccat tacattttga tcgggaccca cttcaaaagc
2101 agccttcatg ccagagatct gtggttattc gaacctttat tactagtgac ttcatgactg
2161 gtatacctgc aacacctggc aatgagatcc ctgtagaggt ggtattaaag atggtcactg
2221 agattaagaa gattcctggt atttctcgaa ttatgtatga cttaacatca aagcccccag
2281 gaactactga gtgggagtaa taaacttc
//