LOCUS AK223350 1655 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for TFIIA-alpha/beta-like factor isoform 1
variant, clone: TST06036.
ACCESSION AK223350
VERSION AK223350.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1655)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..1655
/clone="TST06036"
/clone_lib="TST"
/db_xref="H-InvDB:HIT000331130"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001004042"
/organism="Homo sapiens"
/tissue_type="testis"
CDS <20..1456
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="TFIIA-alpha/beta-like factor isoform 1 variant"
/protein_id="BAD97070.1"
/translation="MACLNPVPKLYRSVIEDVIEGVRNLFAEEGIEEQVLKDLKQLWE
TKVLQSKATEDFFRNSIQSPLFTLQLPHSLHQTLQSSTASLVIPAGRTLPSFATAELG
TSNSSANFTFPGYPIHVPAGVTLQTVSGHLYKVNVPIMVTETSGRAGILQHPIQQVFQ
QLGQPSVIQTSVPQLNPWSLQATTEKSQRIETVLQQPAILPSGPVDRKHLENATSDIL
VSPGNEHKIVPEALLCHQESSHYISLPGVVFSPQVSQTNSNVESVLSGSASMAQNLHD
ESLSTSPHGALHQHVTDIQLHILKNRMYGCDSVKQPRNIEEPSNIPVSEKDSNSQVDL
SIRVTDDDIGEIIQVDGSGDTSSNEEIGSTRDADENEFLGNIDGGDLKVPEEEADSIS
NEDSATNSSDNEDPQVNIVEEDPLNSGDDVSEQDVPDLFDTDNVIVCQYDKIHRSKNK
WKFYLKDGVMCFGGRDYVFAKAIGDAEW"
BASE COUNT 545 a 307 c 345 g 458 t
ORIGIN
1 aggtgctgga ggtgctgtca tggcctgcct caacccggtg cctaaactct acagatctgt
61 aattgaagat gtaattgaag gagttcggaa tctatttgct gaagaaggta tagaggaaca
121 agttttaaaa gacttgaagc agctctggga aaccaaggtt ttgcagtcta aagcaacaga
181 agacttcttc agaaatagca tccaatcacc tctgtttact cttcagttgc cgcacagctt
241 gcaccaaaca ttgcaatcgt caacagcatc attagttatt cccgctggta gaactcttcc
301 aagttttgcc acagcagaac tgggcacttc aaactccagt gcaaacttta cttttcctgg
361 ttatcccatt catgtaccag caggtgtgac actacagact gtatctggtc acctttataa
421 agtcaatgta ccaattatgg tgacagagac ttctggaaga gcaggtattc ttcagcatcc
481 aattcagcaa gtatttcaac agcttggcca gccttcagta atacaaacta gtgttccaca
541 attgaatcca tggtctcttc aagcaactac tgaaaaatca cagagaattg aaaccgtgct
601 acagcaaccc gcaattctac cttctgggcc agtagatagg aaacacttag aaaatgccac
661 cagtgatata cttgtatctc ctggaaatga gcataaaatc gtgcctgaag ctttgttgtg
721 tcatcaggaa agttctcact atatcagtct tccaggtgtt gtattttctc cacaggtctc
781 tcaaacaaat tctaatgtgg agtcagtgct cagtggttca gctagcatgg ctcaaaatct
841 gcatgatgag tccctctcca caagccctca tggggctctc caccagcacg tgactgatat
901 tcagcttcat attcttaaaa ataggatgta tggatgtgat tctgtaaagc aaccaagaaa
961 tatagaggaa cccagcaaca tacctgtatc agagaaggat tctaattctc aggtggattt
1021 aagcattcgg gttactgatg atgatattgg tgaaataatt caagtagatg gaagcggtga
1081 tacatcttcc aatgaagaaa taggaagtac aagagatgca gatgagaatg aatttctagg
1141 gaatattgac gggggagatc tgaaggtacc tgaagaagaa gctgacagta tttcaaatga
1201 ggattcagcc acaaacagta gtgataatga agaccctcaa gtaaacattg tagaagagga
1261 ccctttaaat tctggagatg atgttagtga acaggatgtg ccagacctgt ttgacacgga
1321 taatgtaatt gtctgtcagt atgataagat tcatcgaagc aagaacaaat ggaaattcta
1381 tttgaaagat ggtgttatgt gttttggagg gagagactat gtatttgcaa aagccattgg
1441 tgatgcagag tggtaaacct tgtgagctca gtacatctat tttgtgaaca tcagttggac
1501 tatattgcat attgtgaatt catttttatt ttgaatatag tccagcacag agctgttcaa
1561 atttttagtt cactgtatgg aatttaataa aattataatt cagatgcaga tacaattaca
1621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
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