LOCUS AK223290 1972 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for prosaposin (variant Gaucher disease and
variant metachromatic leukodystrophy) variant, clone: SYN03435.
ACCESSION AK223290
VERSION AK223290.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1972)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..1972
/clone="SYN03435"
/clone_lib="SYN"
/db_xref="H-InvDB:HIT000331070"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001003755"
/organism="Homo sapiens"
/tissue_type="synovial membrane (knee)"
CDS <29..1603
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="prosaposin (variant Gaucher disease and variant
metachromatic leukodystrophy) variant"
/protein_id="BAD97010.1"
/translation="MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGA
VKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNM
SASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIP
ELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIRMVTDIQTAVRTNSTF
VQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKE
MPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKT
EKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTR
LPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQ
CDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTET
AAQCNAVEHCKRHVWN"
BASE COUNT 448 a 520 c 558 g 446 t
ORIGIN
1 attgcagact gcggagtcag acggcgctat gtacgccctc ttcctcctgg ccagcctcct
61 gggcgcggct ctagccggcc cggtccttgg actgaaagaa tgcaccaggg gctcggcagt
121 gtggtgccag aatgtgaaga cggcgtccga ctgcggggca gtgaagcact gcctgcagac
181 cgtttggaac aagccaacag tgaaatccct tccctgcgac atatgcaaag acgttgtcac
241 cgcagctggt gatatgctga aggacaatgc cactgaggag gagatccttg tttacttgga
301 gaagacctgt gactggcttc cgaaaccgaa catgtctgct tcatgcaagg agatagtgga
361 ctcctacctc cctgtcatcc tggacatcat taaaggagaa atgagccgtc ctggggaggt
421 gtgctctgct ctcaacctct gcgagtctct ccagaagcac ctagcagagc tgaatcacca
481 gaagcagctg gagtccaata agatcccaga gctggacatg actgaggtgg tggccccctt
541 catggccaac atccctctcc tcctctaccc tcaggacggc ccccgcagca agccccagcc
601 aaaggataat ggggacgttt gccaggactg cattcggatg gtgactgaca tccagactgc
661 tgtacggacc aactccacct ttgtccaggc cttggtggaa catgtcaagg aggagtgtga
721 ccgcctgggc cctggcatgg ccgacatatg caagaactat atcagccagt attctgaaat
781 tgctatccag atgatgatgc acatgcaacc caaggagatc tgtgcgctgg ttgggttctg
841 tgatgaggtg aaagagatgc ccatgcagac tctggtcccc gccaaagtgg cctccaagaa
901 tgtcatccct gccctggaac tggtggagcc cattaagaag cacgaggtcc cagcaaagtc
961 tgatgtttac tgtgaggtgt gtgaattcct ggtgaaggag gtgaccaagc tgattgacaa
1021 caacaagact gagaaagaaa tactcgacgc ttttgacaaa atgtgctcga agctgccgaa
1081 gtccctgtcg gaagagtgcc aggaggtggt ggacacgtac ggcagctcca tcctgtccat
1141 cctgctggag gaggtcagcc ctgagctggt gtgcagcatg ctgcacctct gctctggcac
1201 gcggctgcct gcactgaccg ttcacgtgac tcagccaaag gacggtggct tctgcgaagt
1261 gtgcaagaag ctggtgggtt atttggatcg caacctggag aaaaacagca ccaagcagga
1321 gatcctggct gctcttgaga aaggctgcag cttcctgcca gacccttacc agaagcagtg
1381 tgatcagttt gtggcagagt acgagcccgt gctgatcgag atcctggtgg aggtgatgga
1441 tccttccttc gtgtgcttga aaattggagc ctgcccctcg gcccataagc ccttgttggg
1501 aactgagaag tgtatatggg gcccaagcta ctggtgccag aacacagaga cagcagccca
1561 gtgcaatgct gtcgagcatt gcaaacgcca tgtgtggaac taggaggagg aatattccat
1621 cttggcagaa accacagcat tggttttttt ctacttgtgt gtctggggga atgaacgcac
1681 agatctgttt gactttgtta taaaaatagg gctcccccac ctcccccatt tctgtgtcct
1741 ttattgtagc attgctgtct gcaagggagc ccctagcccc tggcagacat agctgcttca
1801 gtgccccttt tctctctgct agatggatgt tgatgcactg gaggtctttt agcctgccct
1861 tgcatggcgc ctgctggagg aggagagagc tctgctggca tgagccacag tttcttgact
1921 ggaggccatc aaccctcttg gttgaggcct tgttctgagc cctgacatgt gc
//