LOCUS AK223287 2197 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA, DNA primase large subunit, 58kDa variant,
clone: SYN05993.
ACCESSION AK223287
VERSION AK223287.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2197)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2197
/clone="SYN05993"
/clone_lib="SYN"
/db_xref="H-InvDB:HIT000331067"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001103750"
/organism="Homo sapiens"
/tissue_type="synovial membrane (knee)"
misc_feature 84..782
/product="DNA primase large subunit, 58kDa variant"
BASE COUNT 629 a 359 c 415 g 794 t
ORIGIN
1 tcatatgaac tctcccgcca cccgggaaca gtggctgcca ccgtttgtgt tttcccgagt
61 ttgaattctt gcaggtgacc aagatggagt tttctggaag aaagtggagg aagctgaggt
121 tggcaggtga ccagaggaat gcttcctacc ctcattgcct tcagttttac ttgcagccac
181 cttctgaaaa catatcttta atagaatttg aaaacttggc tattgataga gttaaattgt
241 taaaatcagt tgaaaatctt ggagtgagct atgtgaaagg aactgaacaa taccagagta
301 agttggagag tgagcttcgg aagctcaagt tttcctacag agaaaactta gaagatgaat
361 atgaaccacg aagaagagat catatttctc attttatttt gcggcttgct tattgccagt
421 ctgaagaact tagacgctgg ttcattcaac aagaaatgga tctccttcga tttagattta
481 gtattttacc caaggataaa attcaggatt tcttaaagga tagccaattg cagtttgagg
541 ctataagtga tgaagagaag actcttcgag aacaggagat tgttgcctca tcaccaagtt
601 taagtggact taagttgggg ttcgagtcca tttataagat cccttttgct gatgctctgg
661 atttgtttcg aggaaggaaa gtctatttgg aagatggctt tgcttacgta ccacttaagg
721 acattgtggc aatcatcctg aatgaattta gagccaaact gtccaaggct ttggcagtga
781 gtattttact tgatttctgt atctgacatg ttcacacttt cagttatata cccctggtag
841 ttttattctg tgtttctcta ggaaataagt taattcagtt ttcataatga tattcagatt
901 acatatgatg ttgtactgtt aatcccaaaa ttcctaggtt tttatttgtt actatccata
961 gttggttttt aagttttttt tcttgatcct actaatacca atgctgagtg attaattttg
1021 tgagtaacct ctgtgggttt cttcatggtc atgtcttttt tccttagaaa tagacttcgt
1081 atatggctct taacaattag tgtggcctgt ggtagatata actcctagat ttctgttctg
1141 aattagtatt tggtatcagt agaagacaat atagaagaaa atcttaagta ctaacactgt
1201 gatatgctag tgctgcaatg agtagctcca tactgggtga atttggaggt agctgtgttg
1261 aacaaatgac atgatcagtg atttttaagt agtttgttgg aagtgtgact cttaagtggt
1321 gatatcttaa aactatacac atttttatat gtaaaacaca tattttacag ttgttgtctc
1381 ttaatctgag tcagttttta atactttttt tgtacctaat gcagaagaag acttcaaagg
1441 tttgtgacat ggctgtttta aactgtactg attatagtgt aagcatatgc caagctttcc
1501 ttctcagtta aaaattgtag tattttctga tataaataaa tttttattga ctctctgggg
1561 ttacctaaat atgtataatt tagctatgtc agcacttggg aagactaaaa tactaactct
1621 gtgtttatta aatgaaggaa tttatgagaa cacctagcat aacaggtatt tagtaactgt
1681 taatattctt tctccattcc taatcctgtt atttctttat cctcatgaaa gttgatgctt
1741 ttaatttttt tctttacttg tctgtcacta ccctagatta tattctagtt acagctaaga
1801 atagtgtctt tcaccactgt ggaagacact gatacttgtc tactgtttgg ccattgctct
1861 tttattattc ttagttttat tcacccatat tctctctgtt gacattttag accacacttc
1921 tttgtgtgaa atagacaaat attttccagc atattggata gttttagctg tcatctatta
1981 aatgctggtg gtggacccca gtctccatga caaccaaaaa tatcaccgtg catttcagaa
2041 aacttctggc atgttaatgc tactattaat ctgatcatag tcatttcctt cttaccaaat
2101 ttttaattta tgcctgtgac aatgtcattg tggctaaaaa gtatgagttc tttgggggct
2161 cctgaaaaaa gtttttcttg ctaaaaaaaa aaaaaaa
//