LOCUS AK223282 2203 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for stress 70 protein chaperone,
microsome-associated, 60kDa precursor variant, clone: SYN06447.
ACCESSION AK223282
VERSION AK223282.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2203)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2203
/clone="SYN06447"
/clone_lib="SYN"
/db_xref="H-InvDB:HIT000331062"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001003729"
/organism="Homo sapiens"
/tissue_type="synovial membrane (knee)"
CDS <28..1443
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="stress 70 protein chaperone,
microsome-associated, 60kDa precursor variant"
/protein_id="BAD97002.1"
/translation="MAREMTILGSAVLTLLLAGYLAQQYLPLPTPKVIGIDLGTTYCS
VGVFFPGTGKVKVIPDENGHISIPSMVSFTDNDVYVGYESVELADSNPQNTICDAKRF
IGKIFTAEELEAEIGRYPFKVLNKNGMVEFSVTSNETITVSPEYVGSRLLLKLKEMAE
AYLGMPVANAVISVPAEFDLKQRNSTIEAANLAGLKILRVINEPTAAAMAYGLHKADV
FHVLVIDLGGGTLDVSLLNKQGGMFLTRAMSGNNKLGGQDFNQRLLQYLYKQIYQTYG
FVPSRKEEIHRLRQAVEMVKLNLTLHQSAQLSVLLTVEEQDRKEPHSSDTELPKDKLS
SADDHRVNSGFGRGLSDKKSGESQVLFETEISRKLFDTLNEDLFQKILVPIQQVLKEG
HLEKTEIDEVVLVGGSTRIPRIRQVIQEFFGKDPNTSVDPDLAVVTGVAIQAGIDGGS
WPLQVSALEIPNKHLQKTNFN"
BASE COUNT 685 a 397 c 461 g 660 t
ORIGIN
1 atcacaagcc tgttcggcgg gactgtgatg gccagagaga tgacgatctt aggatcggct
61 gttttgactc tcctgttggc cggctatttg gcacaacagt atttaccatt gcctactcct
121 aaagtgattg gtattgatct tggcaccacc tattgttctg ttggggtgtt ttttcctggc
181 acaggaaaag taaaggtgat tccagatgaa aatgggcata tcagcatacc cagcatggtg
241 tcttttactg acaatgatgt atatgtggga tatgaaagcg tagagctggc agattcaaat
301 cctcaaaaca caatatgtga tgccaaaaga ttcataggca agatttttac cgcagaagag
361 ttggaggctg aaattggcag atacccattt aaggttttaa acaaaaatgg aatggttgag
421 ttttctgtga caagtaatga gaccatcaca gtgtccccag aatatgttgg ctctcgacta
481 ttgttgaagt taaaggaaat ggcagaggca tatcttggaa tgccagttgc caatgctgtc
541 atttctgtac cagcagaatt tgatctaaaa cagagaaatt caacaattga agctgctaac
601 cttgcaggac tgaagatttt gagggtaata aatgaaccca cagcagcagc tatggcctat
661 ggtctccaca aggctgacgt cttccacgtc ttggtgatag acttgggcgg aggaactcta
721 gatgtgtctt tactgaataa acaaggaggg atgtttctaa cccgagcaat gtctggaaac
781 aataaacttg gaggacagga cttcaatcag agattgcttc agtacttata taaacagatc
841 tatcaaacat atggcttcgt gccctctagg aaagaggaaa tccacagatt gagacaagct
901 gtggaaatgg tcaaattaaa tctgactctt catcaatctg ctcagttgtc agtattacta
961 acggtggagg agcaggacag gaaggaacct cacagtagtg acactgaact gccaaaagac
1021 aaactttcct cagcagatga ccatcgcgtg aacagtgggt ttggacgtgg cctttctgat
1081 aagaaaagtg gagaaagtca ggttttattt gaaacagaaa tatcacggaa actctttgat
1141 acccttaatg aagacctctt tcagaaaata ctggtaccca ttcagcaagt attgaaagaa
1201 ggccacctgg aaaagactga gattgatgag gtggttttag ttgggggctc cactcgtatt
1261 cctcggatcc gtcaagtcat tcaagagttc tttggaaaag atcccaacac atctgtagac
1321 cctgacctag cagtagtaac gggagtggct atccaagcag ggattgatgg aggctcttgg
1381 cctctccaag tcagtgcttt agaaattccc aataagcatt tacaaaaaac caacttcaac
1441 tgaattctgc agaaataatg gttatttgtg aacttgtctg atgatctctt cccatttatc
1501 agattacctt ttccacaaaa gaaagtctct aaaatatcac agatttacct agagggcaac
1561 atttagatac aggaaaattt tacatagtgt tttgtcttag gattagacgt gaccagattg
1621 atcctgtttg attttggaga gatcctattc taacaaatac tctaaaatga taaaattgag
1681 gtacaactct cttaaaagag tatggataac tatattttct ggattctgga ggttgataac
1741 catatgcact taacattata ttctataaac attaagtagt gccagttatg agattcccag
1801 ttcttactaa attgtattag caggagctgg taattacttg tattatcaca tgtaactaat
1861 aatttgaact atacttgaag gaccgtgttg atgtcaggta tttacagtgg ttggaagaca
1921 gcagtattat tagcataagc tgcatacgta atattcagta actgccatat tatataacaa
1981 atttacattc acaaattcag tatcctgtta agtgtcatat tcttgtaatc tgcattctcc
2041 aggagtttta tgtgtttaat agatgaattt attttatttc taaaggtatt caaatgtttc
2101 agcaccatat aatagaaata cccaattata ttctagttcc tttatgtcct gtacatcatt
2161 ctctgcttgg atttccatta ttctgtttgg ttagagaata aaa
//