LOCUS AK223214 2006 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for vacuolar protein sorting 45A variant, clone:
STM00427.
ACCESSION AK223214
VERSION AK223214.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2006)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2006
/clone="STM00427"
/clone_lib="STM"
/db_xref="H-InvDB:HIT000330994"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001003419"
/organism="Homo sapiens"
/tissue_type="stomach mucosa"
CDS <19..1731
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="vacuolar protein sorting 45A variant"
/protein_id="BAD96934.1"
/translation="MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEI
LQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVI
SKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQ
GLAALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDR
CDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNM
YLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTV
VGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYA
LHYERHSSNSLPGLMMDLRHKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITK
QFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIG
GATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEVLASGLHSRSKESSQVTSRS
ASRR"
BASE COUNT 596 a 415 c 469 g 526 t
ORIGIN
1 acttgtcaat tcgccgccat gaacgtggtt tttgctgtga agcagtacat ttccaaaatg
61 atagaggaca gcgggcctgg tatgaaagta cttctcatgg ataaagagac gactggcata
121 gtgagtatgg tatacacaca atcggagatt ctacagaagg aagtgtacct ctttgaacgc
181 attgattctc aaaatcgaga gatcatgaaa cacctgaagg caatttgttt tcttcgacct
241 acaaaggaga atgtggatta tattattcag gagctccgaa gacccaaata cactatatat
301 ttcatttatt tcagtaatgt gatcagcaag agtgacgtga agtcattggc tgaagctgat
361 gaacaggaag ttgtggctga ggttcaggaa ttttatggtg attacattgc tgtgaaccca
421 catttgtttt ccctcaatat tttgggttgc tgccagggtc gaaattggga tccagcccag
481 ctatctagaa caactcaagg gcttgcagct ctccttttat ctctgaagaa gtgtcccatg
541 attcgttatc agctctcatc agaggcagca aagagacttg cagagtgcgt taagcaagtg
601 ataactaaag aatatgaact gtttgaattc cgtcggacag aggttcctcc attgctcctt
661 attttagatc gctgtgatga tgccatcacc ccattgctaa accagtggac atatcaggcc
721 atggtccacg aactactagg cataaacaac aatcggattg atctttccag agtgccggga
781 atcagtaaag acttaagaga agtggtccta tctgctgaaa atgatgaatt ctatgctaat
841 aatatgtacc tgaactttgc tgagattggt agcaatataa agaatctcat ggaagatttt
901 cagaagaaga aaccaaaaga acagcaaaaa ctagaatcaa tagcagacat gaaggcgttt
961 gttgagaatt atccacagtt caagaaaatg tctgggactg tttcaaagca tgtgacagtg
1021 gttggagaac tgtctcgatt ggtcagtgaa cggaatctgc tggaggtttc agaggttgag
1081 caagaactgg cctgtcaaaa tgaccattct agtgctctcc agaatataaa aaggcttctg
1141 cagaacccca aagtgacaga gtttgatgct gcccgcctgg tgatgcttta tgctttacat
1201 tatgagcgac acagcagcaa tagcctgcca ggactaatga tggacctcag gcataaaggt
1261 gtttctgaga agtatcgaaa gctcgtgtct gcagttgttg aatatggtgg taaacgagtc
1321 agaggaagtg acctcttcag ccccaaagat gctgtggcta tcaccaaaca attcctcaaa
1381 ggactgaagg gagtagaaaa tgtatataca cagcatcaac ctttcctaca tgaaaccctg
1441 gatcatctca tcaaaggaag gcttaaggaa aacctatatc cttatttagg ccccagcaca
1501 ctcagagaca gacctcagga tatcattgtg tttgtaattg gaggagccac ctatgaagag
1561 gctctaacag tttataacct gaaccgcacc actcctggag tgaggattgt cctgggaggc
1621 accacagtgc acaacacgaa aagtttccta gaggaagttc tggcttctgg actgcacagc
1681 cgaagcaagg agagctctca agtcacatca aggtcagcga gcagaagatg aaacggtggt
1741 tgggggaagg gcacagcttc ctctcttgtc cccactacag gttttcccta ctaaacaaag
1801 gtgttggaga gcagctttgg gttctgtgct ggttgttaga actcatctcc aagtagccca
1861 cggatacgtg gttggcacag acacaagact cccagagttg tcctaacaat aagtctgagc
1921 ccatctcaac ccacttttct ccggtagtct ttatgtatct gttagcacta tcacttcagt
1981 tactgatgaa ttttgttggg atctga
//