LOCUS AK223190 2483 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for Hypothetical protein DKFZp686P18130 variant,
clone: REC00205.
ACCESSION AK223190
VERSION AK223190.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2483)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2483
/cell_type="primary epithelial cells of human renal
proximal tubule"
/clone="REC00205"
/clone_lib="REC"
/db_xref="H-InvDB:HIT000330970"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001003254"
/organism="Homo sapiens"
/tissue_type="renal proximal tubule"
CDS <2..1264
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="Hypothetical protein DKFZp686P18130 variant"
/protein_id="BAD96910.1"
/translation="LGANMAAALRAAGVLLRDPLASSSWRVCQPWRWKSGAAAAAVTT
ETAQHAQGAKPQVQPQKRKPKTGILMLNMGGPETLGDVHDFLLRLFLDRDLMTLPIQN
KLAPFIAKRRTPEIQEQYRRIGGGSPIKIWTSKQGEGMVKLLDELSPNTAPHKYYIGF
RYVHPLTEEAIEEMERDGLERAIAFTQYPQYSCSTTGSSLNAIYRYYNQVGRKPTMKW
STIDRWPTHHLLIQCFADHILKELDHFPLEKRSEVVILFSAHSLPMSVVNRGDPYPQE
VSATVQKVMERLEYCNPYRLVWQSKVGPMPWLGPQTDESIKGLCERGRKNILLVPIAF
TSDHIETLYELDIEYSQVLAKECGVENIRRAESLNGNPLFSKALADLVHSHIQSNELC
SKQLTLSCPLCVNPVCRETKSFFTSQQL"
BASE COUNT 679 a 553 c 575 g 676 t
ORIGIN
1 actcggcgca aacatggctg cggccctgcg cgccgcgggc gtcctgctcc gcgatccgct
61 ggcatccagc agctggaggg tctgtcagcc atggaggtgg aagtcaggtg cagctgcagc
121 ggccgtcacc acagaaacag cccagcatgc ccagggtgca aaacctcaag ttcaaccgca
181 gaagaggaag ccgaaaactg gaatattaat gctaaacatg ggaggccctg aaactcttgg
241 agatgttcac gacttccttc tgagactctt cttggaccga gacctcatga cacttcctat
301 tcagaataag ctggcaccat tcatcgccaa acgccgaacc cccgagattc aagagcagta
361 ccgcaggatt ggaggcggat cccccatcaa gatatggact tccaagcagg gagagggcat
421 ggtgaagctg ctggatgaat tgtcccccaa cacagcccct cacaaatact atattggatt
481 tcggtacgtc catcctttaa cagaagaagc aattgaagag atggagagag atggcctaga
541 aagggctatt gctttcacac agtatccaca gtacagctgc tccaccacag gcagcagctt
601 aaatgccatt tacagatact ataatcaagt gggacggaag cccacgatga agtggagcac
661 tattgacagg tggcccacac atcacctcct catccagtgc tttgcagatc atattctaaa
721 ggaactggac cattttccac ttgagaagag aagcgaggtg gtcattctgt tttctgctca
781 ctcactgccc atgtctgtgg tcaacagagg cgacccatat cctcaggagg taagcgccac
841 tgtccaaaaa gtcatggaaa ggctggagta ctgcaacccc taccgactgg tgtggcaatc
901 caaggttggt ccaatgccct ggttgggtcc tcaaacagac gaatctatca aagggctttg
961 tgagaggggg aggaagaata tcctcttggt tccgatagca tttaccagtg accatattga
1021 aacgctgtat gagctggaca tcgagtactc tcaagtttta gccaaggagt gtggagttga
1081 aaacatcaga agagctgagt ctcttaatgg aaatccattg ttctctaagg ccctggccga
1141 cttggtgcat tcacacatcc agtcaaacga gctgtgttcc aagcagctga ccctgagctg
1201 tccgctctgt gtcaatcctg tctgcaggga gactaaatcc ttcttcacca gccagcagct
1261 gtgacccccg ccggtggacc ccgtggcgtt aggcaaatgc ccaacctcca gatacctccg
1321 atgtggagag ggtgttattt agagatcaag gaaggaagtc atccttcctt gatatatata
1381 cagcctttgg gtacaaattg tgtggtttct tgaggattgg actcttgatg gatttctatt
1441 tttatataac tatacagtaa gcatttgtat tttctctctc taggtataag ttactagttt
1501 ggaatgtcca tcaggacctt taataaatga gttaaaaatt tgtcttatga gacacaccta
1561 tttaagtaca gattttggct ttattgccca aaaccctcct gaaagggtac ggagagtccc
1621 ctctgtgggc tggcagtgtg aatgagatct gtttagtctc gtgcatatag ttgctgtttt
1681 ttaaatgaac acagttgagt atttgaagtg aatttgaaaa agaaatgtta cttaatcttt
1741 ccctaagccc atgggttaca gaatgctagg gaggcaattt ggttacctgc aatggctgct
1801 tttgccagcg aggccaccat tcattggtca tcttggtatt tgtgtgtgaa tctcactttc
1861 ctcaatgtaa aaaggaatca agtatggatt tcagaggtgc tcttagattc cccatacacc
1921 caagggtaat aaacgtgtac aagtacagtg ttcatgatac gtgccttggt gggagtccgt
1981 ggtgccacag ggaaggggct cccactgctt ctggtctcca gggacagtgc tgctggaaag
2041 gctagtgatg agcttcaccc tggagctcct cccgggacct tgcaagcctc tccatccagc
2101 atcttctcta tcttagttga atgccttctt tctgaacatt tgttttaaga attattttat
2161 aaagtcaaca atactttgct tgaattcttt cttaatttac gattttttat tataaaaatg
2221 tatagtgata caatgggaca tgtgaagaat acagaaaagt aaccacttta atgcaataac
2281 tgttatcata atattgtatt tcgtggtagt cctttcctgt agatattttt aatgccattt
2341 aatgccattg tcaccttgga tttatgagtg aaaagtgttt ctaaaaatat agaaataatg
2401 tcagatcaga gtctgatctt ctatgtttgt atttaaatgg attaaaagat ccccggtggt
2461 tccatgaaaa aaaaaaaaaa aaa
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