LOCUS AK223091 2421 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for galactosidase, beta 1 variant, clone:
KAT03749.
ACCESSION AK223091
VERSION AK223091.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2421)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2421
/cell_line="KATO III"
/clone="KAT03749"
/clone_lib="KAT"
/db_xref="H-InvDB:HIT000330871"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001002674"
/organism="Homo sapiens"
CDS <62..2095
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="galactosidase, beta 1 variant"
/protein_id="BAD96811.1"
/translation="MPGFLVRILPLLLVLLLLGPTRGLRNATQRMFEIDYSRDSFLKD
GQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDH
DVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDK
WLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLF
TTDGAHKTLLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDH
WGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYD
APLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDIL
CPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGI
PQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTD
WTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLP
QDTFIQFPGWTRGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWA
PCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV"
BASE COUNT 581 a 636 c 612 g 592 t
ORIGIN
1 agtcaagtga cgcgaagcgg ccggcctggg cgccgactgc agagccggga ggctggtggt
61 catgccgggg ttcctggttc gcatcctccc tctgctgctg gttctgctgc ttctgggccc
121 tacgcgcggc ttgcgcaatg ccacccagag gatgtttgaa attgactata gccgggactc
181 cttcctcaag gatggccagc catttcgcta catctcagga agcattcact actcccgtgt
241 gccccgcttc tactggaagg accggctgct gaagatgaag atggctgggc tgaacgccat
301 ccagacgtat gtgccctgga actttcatga gccctggcca ggacagtacc agttttctga
361 ggaccatgat gtggaatatt ttcttcggct ggctcatgag ctgggactgc tggttatcct
421 gaggcccggg ccctacatct gtgcagagtg ggaaatggga ggattacctg cttggctgct
481 agagaaagag tctattcttc tccgctcctc cgacccagat tacctggcag ctgtggacaa
541 gtggttggga gtccttctgc ccaagatgaa gcctctcctc tatcagaatg gagggccagt
601 tataacagtg caggttgaaa atgaatatgg cagctacttt gcctgtgatt ttgactacct
661 gcgcttcctg cagaagcgct ttcgccacca tctgggggat gatgtggttc tgtttaccac
721 tgatggagca cataaaacac tcctgaaatg tggggccctg cagggcctct acaccacggt
781 ggactttgga acaggcagca acatcacaga tgctttccta agccagagga agtgtgagcc
841 caaaggaccc ttgatcaatt ctgaattcta tactggctgg ctagatcact ggggccaacc
901 tcactccaca atcaagaccg aagcagtggc ttcctccctc tatgatatac ttgcccgtgg
961 ggcgagcgtg aacttgtaca tgtttatagg tgggaccaat tttgcctatt ggaatggggc
1021 caactcaccc tatgcagcac agcccaccag ctacgactat gatgccccac tgagtgaggc
1081 tggggacctc actgagaagt attttgctct gcgaaacatc atccagaagt ttgaaaaagt
1141 accagaaggt cctatccctc catctacacc aaagtttgca tatggaaagg tcactttgga
1201 aaagttaaag acagtgggag cagctctgga cattctgtgt ccctctgggc ccatcaaaag
1261 cctttatccc ttgacattta tccaggtgaa acagcattat gggtttgtgc tgtaccggac
1321 aacacttcct caagattgca gcaacccagc acctctctct tcacccctca atggagtcca
1381 cgatcgagca tatgttgctg tggatgggat cccccaggga gtccttgagc gaaacaatgt
1441 gatcactctg aacataacag ggaaagctgg agccactctg gaccttctgg tagagaacat
1501 gggacgtgtg aactatggtg catatatcaa cgattttaag ggtttggttt ctaacctgac
1561 tctcagttcc aatatcctca cggactggac gatctttcca ctggacactg aggatgcagt
1621 gcgcagccac ctggggggct ggggacaccg tgacagtggc caccatgatg aagcctgggc
1681 ccacaactca tccaactaca cgctcccggc cttttatatg gggaacttct ccattcccag
1741 tgggatccca gacttgcccc aggacacctt tatccagttt cctggatgga ccaggggcca
1801 ggtctggatt aatggcttta accttggccg ctattggcca gcccggggcc ctcagttgac
1861 cttgtttgtg ccccagcaca tcctgatgac ctcggcccca aacaccatca ccgtgctgga
1921 actggagtgg gcaccctgca gcagtgatga tccagaacta tgtgctgtga cgttcgtgga
1981 caggccagtt attggctcat ctgtgaccta cgatcatccc tccaaacctg ttgaaaaaag
2041 actcatgccc ccacccccgc aaaaaaacaa agattcatgg ctggaccatg tatgatgatg
2101 aaagcctgtg tctttgaggg attctaccct gaacatacct cacagatcct ccctgtcatg
2161 ccacatttca ctgattggaa tgtggaaatg gaaaaggaat ttaggatgtg cattttcacc
2221 tgaggtttcc ctgcatccct gcagtgccaa agccccacct tcagggacca cctggaatgt
2281 gtgaggggct gacagcacag taacgtgcat acatatctgc agggctggaa tggaagcttt
2341 aaaggtggta gtgattttta ttttggaaga atcatgttac ctttttgtta aataaaattt
2401 gtactcaaaa aaaaaaaaaa a
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