LOCUS AK222903 2659 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for epidermal growth factor receptor pathway
substrate 8-like protein 2 variant, clone: HRC04418.
ACCESSION AK222903
VERSION AK222903.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2659)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2659
/cell_type="primary human renal epithelial cells"
/clone="HRC04418"
/clone_lib="HRC"
/db_xref="H-InvDB:HIT000330683"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001838"
/organism="Homo sapiens"
/tissue_type="kidney"
CDS <161..2308
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="epidermal growth factor receptor pathway
substrate 8-like protein 2 variant"
/protein_id="BAD96623.1"
/translation="MSQSGAVSCCPGATNGSLGRSDGVAKMSPKDLFEQRKKYSNSNV
IMHETSQYHVQHLATFIMDKSEAITSVDDAIRKLVQLSSKEKIWTQEMLLQVNDQSLR
LLDIESQEELEDFPLPTVQRSQTVLNQLRYPSVLLLVCQDSEQNKPDVHFFHCDEVEA
ELVHEDIESALADCRLGKKMRPQTLKGHQEKIRQRQSILPPPQGPAPIPFQHRGGDSP
EAKNRVGPQVPLSEPGFRRRESQEEPRAVLAQKIEKETQILNCALDDIEWFVARLQKA
AEAFKQLNQRKKGKKKGKKAPAEGVLTLRARPPSEGEFIDCFQKIKLAINLLAKLQKH
IQNPSAAELVHFLFGPLDLIVNTCSGPDIARSVSCPLLSRDAVDFLRGHLVPKEMSLW
ESLGESWMRPRSEWPREPQVPLYVPKFHSGWEPPVDVLQEAPWEVEGLASAPIEEVSP
VSRQSIRNSQKHSPTSEPTPPGDALPPVSSPHTHRGYQPTPAMAKYVKILYDFTARNA
NELSVLKDEVLEVLEDGRQWWKLRSRSGQAGYVPCNILGEARPEDAGAPFEQAGQKYW
GPASPTHKLPPSFPGNKDELMQHMDEVNDELIRKISNIRAQPQRHFRVERSQPVSQPL
TYESGPDEVRAWLEAKAFSPRIVENLGILTGPQLFSLNKEELKKVCGEEGVRVYSQLT
MQKAFLEKQQSGSELEELMNKFHSMNQRRGEDS"
BASE COUNT 556 a 852 c 846 g 405 t
ORIGIN
1 tggcgccccg gccgaggcgc gaacagacgg acgcaccggc gagcgccgag gggacaggcc
61 gagcgcgggg cgccggaggc aggtgtggga caggcactgg cctcagaccg gggccacact
121 gaggtctgcc cttctcccgc tggccgccac ccaagacacc atgagccagt ccggggccgt
181 gagctgctgc ccgggtgcca ccaatggcag cctgggccgg tccgacggtg tggccaagat
241 gagccccaag gacctgtttg agcagaggaa gaagtattcc aactccaacg tcatcatgca
301 cgagacctcg cagtaccacg tccagcacct ggccacattc atcatggaca agagcgaagc
361 catcacgtct gtggacgacg ccatccggaa gctggtgcag ctgagctcca aggagaagat
421 ctggacccag gagatgctgc tgcaggtgaa cgaccagtcg ttgcggctgc tggacatcga
481 gtcacaggag gagctggaag acttcccgct gcccacggtg cagcgcagcc agacggtcct
541 caaccagctg cgctacccgt ctgtgctgct gctcgtgtgc caggactcgg agcagaacaa
601 gccggatgtc cacttcttcc actgcgatga ggtggaggca gagctggtgc acgaggacat
661 cgagagcgcg ttggccgact gccggctggg caagaagatg cggccgcaga ccctgaaggg
721 acaccaggag aagattcggc agcggcagtc catcctgcct cctccccagg gcccggcgcc
781 catccccttc cagcaccgcg gcggggattc cccggaggcc aagaatcgcg tgggcccgca
841 ggtgccactc agcgagccag gtttccgccg tcgggagtcg caggaggagc cgcgggccgt
901 gctggctcag aagatagaga aggagacgca aatcctcaac tgcgccctgg acgacatcga
961 gtggtttgtg gcccggctgc agaaggcagc cgaggctttc aagcagctga accagcggaa
1021 aaaggggaag aagaagggca agaaggcgcc agcagagggc gtcctcacac tgcgggcacg
1081 gcccccctct gagggcgagt tcatcgactg cttccagaaa atcaagctgg cgattaactt
1141 gctggcaaag ctgcagaagc acatccagaa ccccagcgcc gcggagctcg tgcacttcct
1201 cttcgggcct ctggacctga tcgtcaacac ctgcagtggc ccagacatcg cacgctccgt
1261 ctcctgccca ctgctctccc gagatgccgt ggacttcctg cgcggccacc tggtccctaa
1321 ggagatgtcg ctgtgggagt cactgggaga gagctggatg cggccccgtt ccgagtggcc
1381 gcgggagcca caggtgcccc tctacgtgcc caagttccac agcggctggg agcctcctgt
1441 ggatgtgctg caggaggccc cctgggaggt ggaggggctg gcgtctgccc ccatcgagga
1501 ggtgagtcca gtgagccgac agtccataag aaactcccag aagcacagcc ccacttcaga
1561 gcccaccccc ccgggggatg ccctaccacc agtcagctcc ccacatactc acaggggcta
1621 ccagccaaca ccagccatgg ccaagtacgt caagatcctg tatgacttca cagcccgaaa
1681 tgccaacgag ctatcggtgc tcaaggatga ggtcctagag gtgctggagg acggccggca
1741 gtggtggaag ctgcgcagcc gcagcggcca ggcggggtac gtgccctgca acatcctagg
1801 cgaggcgcga ccggaggacg ccggcgcccc gttcgagcag gccggtcaga agtactgggg
1861 ccccgccagc ccgacccaca agctaccccc aagcttcccg gggaacaaag acgagctcat
1921 gcagcacatg gacgaggtca acgacgagct catccggaaa atcagcaaca tcagggcgca
1981 gccacagagg cacttccgcg tggagcgcag ccagcccgtg agccagccgc tcacctacga
2041 gtcgggtccg gacgaggtcc gcgcctggct ggaagccaag gccttcagcc cgcggatcgt
2101 ggagaacctg ggcatcctga ccgggccgca gctcttctcc ctcaacaagg aggagctgaa
2161 gaaagtgtgc ggcgaggagg gcgtccgcgt gtacagccag ctcaccatgc agaaggcctt
2221 cctggagaag cagcaaagtg ggtcggagct ggaagaactc atgaacaagt ttcattccat
2281 gaatcagagg aggggggagg acagctaggc ccagctgcct tgggctgggg cctgcggagg
2341 ggaagcccac ccacaatgca tggagtatta tttttatatg tgtatgtatt ttgtatcaag
2401 gacacggagg gggtgtggtg ctggctagag gtccctgccc ctgtctggag gcacaacgcc
2461 catccttagg ccaaacagta cccaaggcct cagcccacac caagactaat ctcagccaaa
2521 cctgctgctt ggtggtgcca gccccttgtc caccttctct tgaggccaca gaactccctg
2581 gggctggggc ctctttctct ggcctcccct gtgcacctgg ggggtcctgg cccctgtgat
2641 gctcccccat ccccaccca
//