LOCUS AK222887 1958 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for PET112-like variant, clone: HRC00967.
ACCESSION AK222887
VERSION AK222887.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1958)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..1958
/cell_type="primary human renal epithelial cells"
/clone="HRC00967"
/clone_lib="HRC"
/db_xref="H-InvDB:HIT000330667"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001748"
/organism="Homo sapiens"
/tissue_type="kidney"
CDS <4..1677
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="PET112-like variant"
/protein_id="BAD96607.1"
/translation="MAAPMLRWGCRGRRWAFARVDGGSCHRRGAPTGSTSNQIRGESS
VAQQPLHTAQKTRKGEHKWAAVVGLEIHAQISSNSKLFSGSQVRFSAPPNSLVSFFDA
SLPGTLPVLNRRCVEAAVMTGLALNCHINKKSLFDRKHYFYADLPAGYQITQQRLPIA
VNGSLIYGVCAGKKQSQVIPKTVRIKQIQLEQDSGKSLHDNLRSQTLIDLNRAGVGLL
EVVLEPDMSCGEEAATAVRELQLILQALGTSQANMAEGQLRVDANISVHHPGEPLGVR
TEVKNLNSIRFLAKAIDYEIQRQINELENGGEILNETRSFRHKLGCTMSMRDKEGKQD
YRFMPEPNLPPLVLYDATSLPAGADPQQVINIDQIRETLPELPSVTREKLVQQYGMLL
EHSFTLLNEVGLLEFFQNVIKETRAEPKKVTSWVLNTFLGYLKQQNLAVSESPVTPSA
LAELLDLLDSRTISSSAAKQVFEELWKREGKTPGQIVSEKQLELMQDQGALEQLCHSV
MEAHPQVVMDVKDRNPRAINKLIGLVRKATQSRADPVMIKEILEKKLSL"
BASE COUNT 510 a 499 c 538 g 411 t
ORIGIN
1 acaatggcgg cgcccatgct gcgctggggc tgccgtggaa gacgttgggc tttcgcccgg
61 gttgacggtg gttcttgcca ccgaagaggg gctccgactg ggtccacatc caaccagatt
121 aggggagaga gctcagtggc tcagcagccc ctccacacgg cccagaagac gaggaaaggt
181 gaacacaaat gggctgctgt ggtaggtttg gaaattcatg cccagatttc ctccaactct
241 aaactcttct ctggatctca agttcgcttt tcagcacctc caaattcttt ggtttctttt
301 tttgatgcat ctctacctgg aactttgccg gttctcaaca ggaggtgtgt agaagcggcg
361 gtgatgacag gcctggctct gaactgccac ataaacaaga agtccttgtt tgacaggaag
421 cactacttct atgcagacct ccctgcaggc taccaaatta cccagcagag gctcccaatt
481 gctgtgaatg ggagcttgat atatggcgtc tgtgcaggga agaagcagag tcaggtgatc
541 cccaagacgg tgaggatcaa gcagatccag ttggagcaag acagtggcaa aagcctccac
601 gacaacctga ggtctcagac gctcattgat ttgaacaggg caggagtggg ccttctggag
661 gtggtcctgg agcccgacat gtcctgtgga gaagaggcgg caacagctgt cagggagctg
721 cagctgatcc ttcaagccct ggggaccagc caggcgaaca tggcagaggg ccagttgaga
781 gtggatgcca atatatccgt gcatcaccct ggggagcctt tgggcgttcg aacggaagtg
841 aagaatctca acagcatcag gttcctggcc aaagccatag actatgaaat tcagaggcaa
901 atcaatgaac ttgagaatgg aggtgaaatt ctgaacgaaa cacgctcatt tcgtcacaag
961 ctggggtgca ccatgtcaat gagagacaaa gaaggaaaac aggactacag gttcatgcca
1021 gaacccaacc tgcctcccct ggtgctctac gacgccacat ctctgcccgc aggtgcagac
1081 ccacagcaag tgatcaatat tgaccagatt cgggagacac tcccggagct ccccagtgtg
1141 acccgagaga agcttgtcca acagtatggg atgctgctgg aacacagctt cactttgctg
1201 aacgaagtcg gcctactgga gttcttccaa aatgtgataa aagaaactag ggcagagcca
1261 aaaaaggtga ctagttgggt cctcaacact tttctgggct atttaaagca acagaacctc
1321 gctgtcagtg agagtcctgt cacaccctct gcactcgctg agcttcttga cctgctggac
1381 agcagaacaa tttcttcatc agcagctaaa caggtgtttg aggaactgtg gaagagggaa
1441 ggcaagactc cagggcagat tgtttcagaa aagcagcttg aactgatgca ggaccagggg
1501 gcactggagc agctctgcca ctctgtgatg gaggcccatc ctcaagtggt aatggatgtg
1561 aaggacagaa accccagagc tataaataaa ctgattgggt tggtccggaa agcgactcaa
1621 agccgagcag atccagtcat gataaaggag atcctggaga agaagctgtc attgtgagat
1681 gtttgggatc cccttgccca agggacaaca acaaacagtg cagcctgact gggaacagga
1741 tcctgtgaaa gctgatgccc atgtgccctg agagctgcct ctcaatccct gtcccaagcc
1801 acagctatgg cattaatgtc accagtgttc tcaccctcta ggccctgtgc ctggaggtgc
1861 ctccacagcc gaccagcagc caccccgcct gcttcatcca catcaggagg gtccggtgag
1921 gctgcagcag tggttaagga gtaacacctt cttgtatt
//