LOCUS AK222886 2739 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for molybdenum cofactor sulfurase variant,
clone: HEP19329.
ACCESSION AK222886
VERSION AK222886.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2739)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2739
/cell_line="HepG2"
/clone="HEP19329"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000330666"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001742"
/organism="Homo sapiens"
/tissue_type="liver"
CDS <11..2677
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="molybdenum cofactor sulfurase variant"
/protein_id="BAD96606.1"
/translation="MAGAAAESGRELWTFAGSRDPSAPRLAYGYGPGSLRELRAREFS
RLAGTVYLDHAGATLFSQSQLESFTSDLMENTYGNPHSQNISSKLTHDTVEQVRYRIL
AHFHTTAEDYTVIFTAESTAALKLVAEAFPWVSQGPESSGSRFCYLTDSHTSVVGMRN
VTMAINVISIPVRPEDLWSAEERGASASNPDCQLPHLFCYPAQSNFSGVRYPLSWIEE
VKSGRLRPVSTPGKWFVLLDAASYVSTSPLDLSAHQADFVPISFYKIFGFPTGLGALL
VHNRAAPLLRKTYFGGGTASAYLAGEDFYIPRQSVAQRFEDGTISFLDVIALKHGFDT
LERLTGGMENIKQHTFTLAQYTYMALSSLQYPNGAPVVRIYSDSEFSSPEVQGPIINF
NVLDDKGNIIGYSQVDKMASLYNIHLRTGCFCNTGACQRHLGISNEMVRKHFQAGHVC
GDNMDLIDGQPTGSVRISFGYMSTLDDVQAFLRFIIDTRLHSSGDWPVPQAHADTGET
GAPSADSQADVIPAVMGRRSLSPQEDALTGSRVWNNSSTVNAVPVAPPVCDVARTQPT
PSEKAAGVLEGALGPHVVTNLYLYPIKSCAAFEVTRWPVGNQGLLYDRSWMVVNHNGV
CLSQKQEPRLCLIQPFIDLRQRIMVIKAKGMEPIEVPLEENSERTQIRQSRVCADRVS
TYDCGEEISSWLSTFFGRPCNLIKQSSNSQRNAKKKHGKDQLPGTMATLSLVNEAQYL
LINTSSILELHRQLNTSDENGKEELFSLKDLSLRFRANIIINGKRAFEEEKWDEISIG
SLRFQVLGPCHRCQMICIDQQTGQRNQHVFQKLSESRETKVNFGMYLMHASLDLSSPC
FLSVGSQVLPVLKENVEGHDLPASEKHQDVTS"
BASE COUNT 672 a 705 c 717 g 645 t
ORIGIN
1 agccggggcc atggccggcg cggcggcgga gtcagggcgg gagctgtgga ccttcgcggg
61 ttcccgggac ccgagcgcac cgcggctagc ctacggctac ggcccgggca gcctgcgcga
121 gctgcgggcg cgcgagttca gccgcctggc aggaactgtc tatcttgacc atgcaggtgc
181 caccttgttc tcccagagcc agctcgaaag cttcactagt gatctcatgg aaaacactta
241 tggtaatcct cacagccaga acatcagcag caagctcacc catgacactg tggagcaggt
301 gcgctacaga atcctggcgc acttccacac caccgcagaa gactacactg tgatcttcac
361 tgccgagagc acggctgctc tcaaactggt ggcagaggcc tttccatggg tgtcccaggg
421 cccagagagc agtgggagtc gcttctgtta cctcaccgac agccacacct ccgtagtggg
481 tatgaggaac gtgaccatgg ctataaatgt catatccatc ccggtcaggc cagaggacct
541 gtggtctgca gaggaacgtg gtgcttcagc cagcaaccca gactgccagc tgccgcatct
601 cttctgctac ccagctcaga gtaacttttc tggagtcaga taccccctgt cctggataga
661 agaggtcaag tctgggcggt tgcgccctgt gagcacgcct gggaagtggt ttgtgctgct
721 ggatgcagcc tcctacgtga gcacctcgcc tttggacctg tcagctcacc aggccgactt
781 tgtccccatc tccttctata agatcttcgg gtttcctaca ggcctgggcg ctctgctggt
841 ccataatcgt gcggctcctc tactgaggaa gacctacttt ggaggaggga cagcctctgc
901 gtacctagca ggagaagact tctacatccc gaggcagtcg gtagctcaga ggtttgaaga
961 tggcaccatc tcattccttg atgttatcgc gctaaaacat ggatttgaca ccctagagcg
1021 cctcacaggt ggaatggaga atataaagca gcacaccttc accttggctc agtataccta
1081 catggccctg tcctctctcc agtaccccaa tggagcccct gtggtgcgga tttacagcga
1141 ttctgagttc agcagccctg aggttcaggg cccgatcatc aattttaatg tgctggatga
1201 caaagggaac atcattggtt actcccaggt ggacaaaatg gccagtcttt acaacatcca
1261 cctgcgaact ggctgcttct gtaacactgg ggcctgccag aggcacctgg gcataagcaa
1321 cgagatggtc aggaagcatt ttcaggctgg tcatgtctgt ggggacaata tggacctcat
1381 agatgggcag cccacaggat ctgtgaggat ttcatttgga tacatgtcga cgctggatga
1441 tgtccaggcc tttcttaggt tcatcataga cactcgcctg cactcatcag gggactggcc
1501 tgtccctcag gcccatgctg acaccgggga gactggagcc ccatcagcag acagccaggc
1561 tgatgttata cctgctgtca tgggcagacg tagcctctcg cctcaggaag atgccctcac
1621 aggctccagg gtttggaaca actcgtctac tgtgaatgct gtgcctgtgg ccccacctgt
1681 gtgtgatgtc gccagaaccc agccgactcc ttcagagaaa gctgcaggag tcctggaggg
1741 ggcccttggg ccacatgttg tcactaacct ttatctctat ccaatcaaat cctgtgctgc
1801 atttgaggtg accaggtggc ctgtaggaaa ccaagggctg ctatatgacc ggagctggat
1861 ggttgtgaat cacaatggtg tttgcctgag tcagaagcag gaaccccggc tctgcctgat
1921 ccagcccttc atcgacttgc ggcaaaggat catggtcatc aaagccaaag ggatggagcc
1981 tatagaggtg cctcttgagg aaaatagtga acggactcag attcgccaaa gcagggtctg
2041 tgctgacaga gtaagtactt atgattgtgg agaagaaatt tcaagctggt tgtcaacatt
2101 ttttggccgt ccttgtaatt tgatcaaaca aagttcaaac tctcaaagga atgcaaagaa
2161 gaaacatgga aaagatcaac ttcctggtac aatggccacc ctttctctgg tgaatgaggc
2221 acagtatctg ctgatcaaca catccagtat tttggaactt caccggcaac taaacaccag
2281 tgatgagaat ggaaaggagg aattattctc actgaaggat ctcagcttgc gttttcgtgc
2341 caatattatt atcaatggaa aaagggcttt tgaagaagag aaatgggatg agatttcaat
2401 tggctctttg cgtttccagg ttttggggcc ttgtcacaga tgccagatga tttgcatcga
2461 ccagcaaact gggcaacgaa accagcatgt tttccaaaaa ctttctgaga gtcgtgaaac
2521 aaaggtgaac tttggcatgt acctgatgca tgcatcattg gatttatcct ccccatgttt
2581 cctgtctgta ggatctcagg tgctccctgt gttgaaagag aatgtggaag gtcatgattt
2641 acctgcatct gagaaacacc aggatgttac ctcctaaaaa aaatttttag catacattaa
2701 agtttctctt ttacagtgaa aaaaaaaaaa aaaaaaaaa
//