LOCUS AK222876 2274 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for ubiquitin conjugating enzyme 7 interacting
protein 5 isoform a variant, clone: HEP15307.
ACCESSION AK222876
VERSION AK222876.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2274)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2274
/cell_line="HepG2"
/clone="HEP15307"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000330656"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001702"
/organism="Homo sapiens"
/tissue_type="liver"
CDS <112..1737
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="ubiquitin conjugating enzyme 7 interacting
protein 5 isoform a variant"
/protein_id="BAD96596.1"
/translation="MVINLCLPQFRPRIHCNKISADGYEVENLISEDLTKRSHGFRTE
YFIKPPVYVTVSFPFNVEICRINIDLTAGGGQNVTGLEMYTSASSSRVSWNTPQCRTL
GPAEPSVPDKEAFTLVGKVLLKNQSQVVFSHRGFKARPPFGAMEATLPSPAVVAQELW
NKGALSLSHVAHLRICITHVTGGGIPCIKRLEVWGQPAKTCSQEVIDSILLVTSENLP
QDVALQAPALPMESDCDPGDQPESQQAPSSLQKLAEIIQDVPEEFLDPITLEIMPCPM
LLPSGKVIDQSTLEKCNRSEATWGRVPSDPFTGVAFTPHSQPLPHPSLKARIDHFLLQ
HSIPGCHLLGRAQTALAVIPSSIVLPSQKRKIEQAEHVPDSNFGVNASCFSATSPLVL
PTTSEHTAKKMKATNEPSLTHMDCSTGPLSHEQKLSQSLEIALASTLGSMPSFTARLT
RGQLQHLGTRGSNTSWRPGTGSEQPGSILGPECASCKRAFSPYFKKEPVYQLPCGHLL
CRPCLGEKQRSLPMTCTACQRPVASQDVLRVHF"
BASE COUNT 524 a 694 c 598 g 458 t
ORIGIN
1 agtatctgtg tcgccgccgc ccgcggcgtc cccggtttgg tgttgcggcg cccaccttcg
61 ggaggatcag gctgcttctg atgcttggaa gatatcctct cagccacaaa gatggtaata
121 aatctttgcc tcccacagtt cagaccaaga attcactgca acaagatatc agctgatggt
181 tacgaagtag aaaatctcat ctctgaagat ctcacaaaga gaagtcatgg tttcaggaca
241 gagtatttca ttaagccacc agtctatgtg acagtttcat ttccctttaa tgtggaaatc
301 tgtaggatca acatagacct cacagctggg ggaggtcaga acgtcactgg cctggaaatg
361 tacacatctg cctcatctag cagagtgtct tggaatacgc cccagtgccg gaccctgggc
421 ccagctgagc catctgtccc agacaaggag gcgttcacct tggtaggcaa agtcttactg
481 aaaaaccaga gccaagtggt gtttagccac aggggcttca aggccaggcc cccttttggc
541 gcgatggaag ccacactccc ctcccctgct gttgtggccc aggagctctg gaataaaggg
601 gctctttccc ttagccacgt ggcccactta aggatctgta tcacccatgt gacaggcggc
661 ggtatccctt gtatcaagcg gttggaagtg tggggtcagc cggccaagac ctgctcccag
721 gaagtgatag acagcatcct gctggtcacc tcagagaacc tgcctcagga tgtggctctg
781 caggctccag ccttgcccat ggaaagtgac tgtgaccctg gggaccagcc tgagagccag
841 caggctccct ccagcctgca gaagctggcc gagatcattc aggatgtgcc tgaggagttc
901 ctggatccca tcaccctgga gatcatgcct tgtcccatgc tgctgccctc aggcaaggtc
961 atcgaccaga gcacactgga gaagtgtaac cgcagtgaag ccacatgggg ccgagtgccc
1021 agtgaccctt tcacgggggt agcttttact ccgcactctc agcccctgcc tcacccctcc
1081 ctcaaggccc ggattgacca tttcctgctc cagcactcca tccctggctg ccacctgctt
1141 gggagagcac agacggcatt ggcagtgatc ccttcttcca ttgttctgcc ctctcagaaa
1201 aggaagatag agcaggctga acatgtccca gacagtaact ttggtgtaaa tgcttcctgt
1261 ttttctgcca caagcccttt ggtcttaccc actacctcag agcacactgc taagaaaatg
1321 aaagccacca atgagcccag cctgacacat atggactgct cgacaggtcc actgtcccac
1381 gagcagaagc tgtcacaaag cttggaaatt gccttggcat ccacccttgg ctctatgccc
1441 tccttcacgg cacggctgac caggggacag ctccagcacc ttggcacaag agggagcaac
1501 acttcctgga ggcctggcac cggctcggag cagcctggga gcatcctggg ccccgaatgt
1561 gcctcctgca aaagagcatt ttctccctac ttcaaaaagg agccggtgta ccagctgccc
1621 tgcggccacc tcctgtgccg accctgcctg ggtgagaagc aacgctccct gcccatgacg
1681 tgcacagcct gccagcggcc ggttgctagc caagacgtgc tgcgggtcca cttctgagtg
1741 actgacctcc actggaggag acccattgct gggaggagct gagggggaac aggagcaggg
1801 ccacagcacc cctgaggtct ggccaggccc caggcacaga gctgcctgct ccctcccggg
1861 gctcttcttc atcacctcac ggtatagcac attgcttctg cgctggtggc aatagggcaa
1921 caaagccata ggccagaggg cggggggatg tccctgcctc cctgccaccc cccctgcctg
1981 agcccaggac ccactggagc cagccccacc ctaggcagga agacccctgc tgagggcccc
2041 cccgtgcagt ccgcataccc ccctgtccag cagggcactg tgggtggctc accctagatt
2101 gtggcccaga tctcaggagt ctctgccttc agggtcatcc aaaagtggac cttgggagca
2161 gtgggggtgt ctgtggagtg catgactcag ccccccgact cgcagcctta ataaagcgat
2221 ggttgacgtc taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//