LOCUS AK222860 3007 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for mutS homolog 2 variant, clone: HEP15490.
ACCESSION AK222860
VERSION AK222860.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3007)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..3007
/cell_line="HepG2"
/clone="HEP15490"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000330640"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001650"
/organism="Homo sapiens"
/tissue_type="liver"
CDS <63..>2696
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="mutS homolog 2 variant"
/protein_id="BAD96580.1"
/translation="MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYT
AHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEV
YKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQ
VGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQ
RGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNSAVLPEMENQVAVSSLSAVIKF
LELLSDDSNFGQFELTTFDFSQYMKLDIAAVRALNLFQGSVEDTTGSQSLAALLNKCK
TPQGQRLVNQWIKQPLMDKNRIEERLNLVEAFVEDAELRQTLQEDLLRRFPDLNRLAK
KFQRQAANLQDCYRLYQGINQLPNVIQALEKHEGKHQKLLLAVFVTPLTDLRSDFSKF
QEVIETTLDMDQVENHEFLVKPSFDPNLSELREIMNDLEKKMQSTLISAARDLGLDPG
KQIKLDSSAQFGYYFRVTCKEEKVLRNNKNFSTVDIQKNGVKFTNSKLTSLNEEYTKN
KTEYEEAQDAIVKEIVNISSGYVEPMQTLNDVLAQLDAVVSFAHVSNGAPVPYVRPAI
LEKGQGRIILKASRHACVEVQDEIAFIPNDVYFEKDKQMFHIITGPNMGGKSTYIRQT
GVIVLMAQIGCFVPCESAEVSIVDCILARVGAGDSQLKGVSTFMAEMLETASILRSAT
KDSLIIIDELGRGTSTYDGFGLAWAISEYIATKIGAFCMFATHFHELTALANQIPTVN
NLHVTALTTEETLTMLYQVKKGVCDQSFGIHVAELANFPKHVIECAKQKALELEEFQY
IGESQGYDIMEPAAKKCYLERE"
BASE COUNT 943 a 555 c 710 g 799 t
ORIGIN
1 aaacagctta gtgggtgtgg ggtcgcgcat tttcttcaac caggaggtga ggaggtttcg
61 acatggcggt gcagccgaag gagacgctgc agttggagag cgcggccgag gtcggcttcg
121 tgcgcttctt tcagggcatg ccggagaagc cgaccaccac agtgcgcctt ttcgaccggg
181 gcgacttcta tacggcgcac ggcgaggacg cgctgctggc cgcccgggag gtgttcaaga
241 cccagggggt gatcaagtac atggggccgg caggagcaaa gaatctgcag agtgttgtgc
301 ttagtaaaat gaattttgaa tcttttgtaa aagatcttct tctggttcgt cagtatagag
361 ttgaagttta taagaataga gctggaaata aggcatccaa ggagaatgat tggtatttgg
421 catataaggc ttctcctggc aatctctctc agtttgaaga cattctcttt ggtaacaatg
481 atatgtcagc ttccattggt gttgtgggtg ttaaaatgtc cgcagttgat ggccagagac
541 aggttggagt tgggtatgtg gattccatac agaggaaact aggactgtgt gaattccctg
601 ataatgatca gttctccaat cttgaggctc tcctcatcca gattggacca aaggaatgtg
661 ttttacccgg aggagagact gctggagaca tggggaaact gagacagata attcaaagag
721 gaggaattct gatcacagaa agaaaaaaag ctgacttttc cacaaaagac atttatcagg
781 acctcaaccg gttgttgaaa ggcaaaaagg gagagcagat gaatagtgct gtattgccag
841 aaatggagaa tcaggttgca gtttcatcac tgtctgcggt aatcaagttt ttagaactct
901 tatcagatga ttccaacttt ggacagtttg aactgactac ttttgacttc agccagtata
961 tgaaattgga tattgcagca gtcagagccc ttaacctttt tcagggttct gttgaagata
1021 ccactggctc tcagtctctg gctgccttgc tgaataagtg taaaacccct caaggacaaa
1081 gacttgttaa ccagtggatt aagcagcctc tcatggataa gaacagaata gaggagagat
1141 tgaatttagt ggaagctttt gtagaagatg cagaattgag gcagacttta caagaagatt
1201 tacttcgtcg attcccagat cttaaccgac ttgccaagaa gtttcaaaga caagcagcaa
1261 acttacaaga ttgttaccga ctctatcagg gtataaatca actacctaat gttatacagg
1321 ctctggaaaa acatgaagga aaacaccaga aattattgtt ggcagttttt gtgactcctc
1381 ttactgatct tcgttctgac ttctccaagt ttcaggaagt gatagaaaca actttagata
1441 tggatcaggt ggaaaaccat gaattccttg taaaaccttc atttgatcct aatctcagtg
1501 aattaagaga aataatgaat gacttggaaa agaagatgca gtcaacatta ataagtgcag
1561 ccagagatct tggcttggac cctggcaaac agattaaact ggattccagt gcacagtttg
1621 gatattactt tcgtgtaacc tgtaaggaag aaaaagtcct tcgtaacaat aaaaacttta
1681 gtactgtaga tatccagaag aatggtgtta aatttaccaa cagcaaattg acttctttaa
1741 atgaagagta taccaaaaat aaaacagaat atgaagaagc ccaggatgcc attgttaaag
1801 aaattgtcaa tatttcttca ggctatgtag aaccaatgca gacactcaat gatgtgttag
1861 ctcagctaga tgctgttgtc agctttgctc acgtgtcaaa tggagcacct gttccatatg
1921 tacgaccagc cattttggag aaaggacaag gaagaattat attaaaagca tccaggcatg
1981 cttgtgttga agttcaagat gaaattgcat ttattcctaa tgacgtatac tttgaaaaag
2041 ataaacagat gttccacatc attactggcc ccaatatggg aggtaaatca acatatattc
2101 gacaaactgg ggtgatagta ctcatggccc aaattgggtg ttttgtgcca tgtgagtcag
2161 cagaagtgtc cattgtggac tgcatcttag cccgagtagg ggctggtgac agtcaattga
2221 aaggagtctc cacgttcatg gctgaaatgt tggaaactgc ttctatcctc aggtctgcaa
2281 ccaaagattc attaataatc atagatgaat tgggaagagg aacttctacc tacgatggat
2341 ttgggttagc atgggctata tcagaataca ttgcaacaaa gattggtgct ttttgcatgt
2401 ttgcaaccca ttttcatgaa cttactgcct tggccaatca gataccaact gttaataatc
2461 tacatgtcac agcactcacc actgaagaga ccttaactat gctttatcag gtgaagaaag
2521 gtgtctgtga tcaaagtttt gggattcatg ttgcagagct tgctaatttc cctaagcatg
2581 taatagagtg tgctaaacag aaagccctgg aacttgagga gtttcagtat attggagaat
2641 cgcaaggata tgatatcatg gaaccagcag caaagaagtg ctatctggaa agagagaacc
2701 tcagagtgac agagccaaaa gaccagtgcc tcattttgct gacatggaaa aggaaacttc
2761 gtgggggaaa gagatctgct tgcagtcggc cagagagaca gaaccagggc agtggtgagc
2821 tctcatgacc tggtgtctgt tgccttctgg ttaagttttt catttgtaat tctacaaaca
2881 tcccttctgt aaacatttcc ctcaaaatgg agcaggaagc tctcaaaaat ggaccagaaa
2941 ggggtcagga atataacttt ctctgcccag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3001 aaaaaaa
//