LOCUS AK222812 1968 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for interferon-alpha receptor 1 precursor
variant, clone: HEP04602.
ACCESSION AK222812
VERSION AK222812.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1968)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..1968
/cell_line="HepG2"
/clone="HEP04602"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000330592"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001467"
/organism="Homo sapiens"
/tissue_type="liver"
CDS <84..1757
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="interferon-alpha receptor 1 precursor variant"
/protein_id="BAD96532.1"
/translation="MMVVLLGATTLVLVAVAPWVLSAAAGGKNLKSPQKVEVDIIDDN
FILRWNRSDESVGNMTFSFDYQKTGMDNWIKLSGCQNITSTKCNFSSLKLNVYEEIKL
RIRAEKENTSSWYEVDSFTPFRKAQIGPPEVHLEAEDKAIVIHISPGTKDSVMWALDG
LSFTYSLLIWKNSSGVEERIENIYSRHKIYKLSPETTYCLKVKAALLTSWKIGVYSPV
HCIKTTVENELPPPENIEVSVQNQNYVLKWDYTYANMTFQVQWLHAFLKRNPGNHLYK
WKRIPDCENVKTTQCVFPQNVFQKGIYLLRVQASDGNNTSFWSEEIKFDTEIQAFLLP
PVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPLIYEIIFWENTSNAERKIIEKKTDVT
VPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVCEKTKPGNTSKIWLIVGICIALFAL
PFVIYAAKVFLRCINYVFFPSLKPSSSINEYFSEQPLKNLLLSTSEEQIEKCFIIENI
STIATVEETNQTDEDHKKYSSQTSQDSGNYSNEDESESKTSEELQQDFV"
BASE COUNT 654 a 371 c 393 g 550 t
ORIGIN
1 gtgacttagg acggggcgat ggcggctgag aggagctgcg cgtgcgcgaa catgtaactg
61 gtgggatctg cggcggctcc cagatgatgg tcgtcctcct gggcgcgacg accctagtgc
121 tcgtcgccgt ggcgccatgg gtgttgtccg cagccgcagg tggaaaaaat ctaaaatctc
181 ctcaaaaagt agaggtcgac atcatagatg acaactttat cctgaggtgg aacaggagcg
241 atgagtctgt cgggaatatg actttttcat tcgattatca aaaaactggg atggataatt
301 ggataaaatt gtctgggtgt cagaatatta ctagtaccaa atgcaacttt tcttcactca
361 agctgaatgt ttatgaagaa attaaattgc gtataagagc agaaaaagaa aacacttctt
421 catggtatga ggttgactca tttacaccat ttcgcaaagc tcagattggt cctccagaag
481 tacatttaga agctgaagat aaggcaatag tgatacacat ctctcctgga acaaaagata
541 gtgttatgtg ggctttggat ggtttaagct ttacatatag cttacttatc tggaaaaact
601 cttcaggtgt agaagaaagg attgaaaata tttattccag acataaaatt tataaactct
661 caccagagac tacttattgt ctaaaagtta aagcagcact acttacgtca tggaaaattg
721 gtgtctatag tccagtacat tgtataaaga ccacagttga aaatgaacta cctccaccag
781 aaaatataga agtcagtgtc caaaatcaga actatgttct taaatgggat tatacatatg
841 caaacatgac ctttcaagtt cagtggctcc acgccttttt aaaaaggaat cctggaaacc
901 atttgtataa atggaaacga atacctgact gtgaaaatgt caaaactacc cagtgtgtct
961 ttcctcaaaa cgttttccaa aaaggaattt accttctccg cgtacaagca tctgatggaa
1021 ataacacatc tttttggtct gaagagataa agtttgatac tgaaatacaa gctttcctac
1081 ttcctccagt ctttaacatt agatccctta gtgattcatt ccatatctat atcggtgctc
1141 caaaacagtc tggaaacacg cctgtgatcc aggattatcc actgatttat gaaattattt
1201 tttgggaaaa cacttcaaat gctgagagaa aaattatcga gaaaaaaact gatgttacag
1261 ttcctaattt gaaaccactg actgtatatt gtgtgaaagc cagagcacac accatggatg
1321 aaaagctgaa taaaagcagt gtttttagtg acgctgtatg tgagaaaaca aaaccaggaa
1381 atacctctaa aatttggctt atagttggaa tttgtattgc attatttgct ctcccgtttg
1441 tcatttatgc tgcgaaagtc ttcttgagat gcatcaatta tgtcttcttt ccatcactta
1501 aaccttcttc cagtataaat gagtatttct ctgaacagcc attgaagaat cttctgcttt
1561 caacttctga ggaacaaatc gaaaaatgtt tcataattga aaatataagc acaattgcta
1621 cagtagaaga aactaatcaa actgatgaag atcataaaaa atacagttcc caaactagcc
1681 aagattcagg aaattattct aatgaagatg aaagcgaaag taaaacaagt gaagaactac
1741 agcaggactt tgtatgacca gaaatgaact gtgtcaagta taaggttttt cagcaggagt
1801 tacactggga gcctgaggtc ctcaccttcc tctcagtaac tacagagagg acgtttccct
1861 gtttagggaa agaaaaaaca tcttcagatc ataggtccta aaaatacggg caagctctta
1921 actatttaaa aatgaaatta caggcccggg cacggtggct cacacctg
//